Wyniki wyszukiwania

1

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

100%

Ryszawy D., Podulka J., Tworzydlo A., Tomassy J., Jarmulski M., Madeja Z., Korohoda W.

Folia Biologica

|

2019

|

tom 67

|

nr 4

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The influence of the term of excision and the explant type on Fritillaria persica L. regeneration in vitro

100%

Marcinek B., Swistowska A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

3

High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh

100%

Gharari Z., Bagheri K., Sharari A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2022

|

tom 103

|

nr 2

4

Initiation and origin of stigma-like structures [SLS] on ovary and style explants of saffron in tissue culture

100%

Namin M. H., Ebrahimzadeh H., Ghareyazie B., Radjabian T., Namin H. H.

Acta Biologica Cracoviensia. Series Botanica

|

2010

|

tom 52

|

nr 1

55-60

Saffron, made from the dried stigmas of Crocus sativus L., contains pigments and valuable aromatic compounds, and can be used in medicine and as a spice. Nowadays its production is lower than demand. Tissue culture presents an alternative biochemical tool which can be used to produce stigma-like structure (SLS) in vitro. In this study, the origin and induction of SLS formation was investigated in ovary and style explants of floral buds on MS medium supplemented with 1-naphthalene acetic acid (NAA) and 6-benzlaminopurine (BAP). SLS were directly originated through meristematic cells or indirectly in the form of colorless globular structures from parenchyma tissue. The colorless globular structures initially were conical and pale yellow color at the sharp ends; subsequently they matured into trumpet-like red stigmas with or without finger-like papillae at the margins. Light and electron microscopic observations of ultra- and semithin sections of different developmental stages of SLS showed that these structures possess two kinds of cells: (1) small cells close to parenchyma tissues and (2) large cells oriented towards the peripheral area and apparently originated from the small ones. Our results suggest that the SLS originated from internal parenchyma tissues.

5

Callus induction and subsequent plant regeneration from leaf explants of Gerbera jamesonii

100%

Parthasarathy V. A., Parthasarathy U., Nagaraju V., Mishra M.

Folia Horticulturae

|

1997

|

tom 09

|

nr 2

6

Micropropagation of Asparagus densiflorus Jesson cv. Myriocladus

100%

Pindel A.

Folia Horticulturae

|

1995

|

tom 07

|

nr 2

7

Regeneration of Pelargonium x hederaefolium 'Bonete' from petiole explants

100%

Wojtania A., Gabryszewska E., Marasek A.

Acta Physiologiae Plantarum

|

2004

|

tom 26

|

nr 3

8

Regeneration of Cymbidium orchids from leaf and root explants

100%

Pindel A., Miczynski K.

Folia Horticulturae

|

1996

|

tom 08

|

nr 2

9

In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

84%

Kasprzyk-Pawelec A., Pietrusiewicz J., Szczuka E.

Acta Scientiarum Polonorum. Hortorum Cultus

|

2015

|

tom 14

|

nr 4

In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t

10

Medicago truncatula: A model plant for studying somatic embryogenesis and organogenesis in legumes

84%

Imin N., Nizamidin M., Schultz N., Holmes P., Weinman J. J., de Jong F., Nolan K. E., Rose R. J., Rolfe B. G.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2005

|

tom 47

|

nr 1

11

Morphogenic responses of Arabidopsis explants cultured in vitro in relation to the level of oxidative stress

84%

Kudelko K., Gaj M. D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

12

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

84%

Pilarska M., Popielarska-Konieczna M., Slesak H., Kozieradzka-Kiszkurno M., Goralski G., Konieczny R., Bohdanowicz J., Kuta E.

Acta Societatis Botanicorum Poloniae

|

2014

|

tom 83

|

nr 1

Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

13

Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of indole-3-acetic acid

84%

Wegrzynowicz-Lesiak E., Saniewski M., Goraj J., Horbowicz M., Miyamoto K., Ueda J.

Journal of Fruit and Ornamental Plant Research

|

2012

|

tom 20

|

nr 1

14

Endogenous hormone concentrations in Arabidopsis explants undergoing induction of somatic embryogenesis

84%

Nowak K., Wojcikowska B., Kiwior-Wesolowska A., Wojcik A., Grzybkowska D., Gaj M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2014

|

tom 56

|

nr 1

15

In vitro responses of various explants of Fagus sylvatica

84%

Hazubska-Przybyl T., Chmielarz P., Bojarczuk K.

Dendrobiology

|

2015

|

tom 73

There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.

16

In vitro regeneration of bulblets from scale explants of Hippeastrum x chmielii (Chm.) in the solid and liquid medium

84%

Witomska M., Latkowska M. J.

Annals of Warsaw Agricultural University. Horticulture and Landscape Architecture

|

2005

|

tom 26

17

Tissue disinfection for preparation of Dendrobium in vitro culture

84%

Teixeira da Silva J. A., Winarto B., Dobranszki J., Cardoso J. C., Zeng S.

Folia Horticulturae

|

2016

|

tom 28

|

nr 1

18

In vitro regeneration of valerian (Valeriana officinalis L.) induced by different concentrations of NAA and BAP

84%

Mahmoodzadeh H., Fatehi H.

Electronic Journal of Polish Agricultural Universities. Series Biology

|

2016

|

tom 19

|

nr 1

19

Date palm (Phoenix dactylifera L.) biotechnology: a mini-review

84%

Khokhar M. I., Teixeira da Silva J. A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2017

|

tom 98

|

nr 2

20

In vitro regeneration abilities of bulb explants of Lachenalia 'Romaud' on the initiation culture stage

84%

Bach A., Kapczynska A.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2014

|

tom 56

|

nr 1

Ograniczanie wyników

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

The influence of the term of excision and the explant type on Fritillaria persica L. regeneration in vitro

High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh

Initiation and origin of stigma-like structures [SLS] on ovary and style explants of saffron in tissue culture

Callus induction and subsequent plant regeneration from leaf explants of Gerbera jamesonii