Wyniki wyszukiwania

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Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

100%

Ryszawy D., Podulka J., Tworzydlo A., Tomassy J., Jarmulski M., Madeja Z., Korohoda W.

Folia Biologica

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2019

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tom 67

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nr 4

2

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The influence of the term of excision and the explant type on Fritillaria persica L. regeneration in vitro

100%

Marcinek B., Swistowska A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

3

High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh

100%

Gharari Z., Bagheri K., Sharari A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2022

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tom 103

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nr 2

4

Callus induction and subsequent plant regeneration from leaf explants of Gerbera jamesonii

100%

Parthasarathy V. A., Parthasarathy U., Nagaraju V., Mishra M.

Folia Horticulturae

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1997

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tom 09

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nr 2

5

Micropropagation of Asparagus densiflorus Jesson cv. Myriocladus

100%

Pindel A.

Folia Horticulturae

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1995

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tom 07

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nr 2

6

Regeneration of Pelargonium x hederaefolium 'Bonete' from petiole explants

100%

Wojtania A., Gabryszewska E., Marasek A.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3

7

Regeneration of Cymbidium orchids from leaf and root explants

100%

Pindel A., Miczynski K.

Folia Horticulturae

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1996

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tom 08

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nr 2

8

In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

84%

Kasprzyk-Pawelec A., Pietrusiewicz J., Szczuka E.

Acta Scientiarum Polonorum. Hortorum Cultus

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2015

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tom 14

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nr 4

In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t

9

Medicago truncatula: A model plant for studying somatic embryogenesis and organogenesis in legumes

84%

Imin N., Nizamidin M., Schultz N., Holmes P., Weinman J. J., de Jong F., Nolan K. E., Rose R. J., Rolfe B. G.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

10

Morphogenic responses of Arabidopsis explants cultured in vitro in relation to the level of oxidative stress

84%

Kudelko K., Gaj M. D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

11

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

84%

Pilarska M., Popielarska-Konieczna M., Slesak H., Kozieradzka-Kiszkurno M., Goralski G., Konieczny R., Bohdanowicz J., Kuta E.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 1

Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

12

Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of indole-3-acetic acid

84%

Wegrzynowicz-Lesiak E., Saniewski M., Goraj J., Horbowicz M., Miyamoto K., Ueda J.

Journal of Fruit and Ornamental Plant Research

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2012

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tom 20

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nr 1

13

Endogenous hormone concentrations in Arabidopsis explants undergoing induction of somatic embryogenesis

84%

Nowak K., Wojcikowska B., Kiwior-Wesolowska A., Wojcik A., Grzybkowska D., Gaj M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

14

In vitro responses of various explants of Fagus sylvatica

84%

Hazubska-Przybyl T., Chmielarz P., Bojarczuk K.

Dendrobiology

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2015

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tom 73

There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.

84%

Sandhya H., Srinath R.

International Letters of Natural Sciences

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2015

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tom 44

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α- naphthalene acetic acid (NAA) and Indole- 3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.

Ograniczanie wyników

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

The influence of the term of excision and the explant type on Fritillaria persica L. regeneration in vitro

High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh

Callus induction and subsequent plant regeneration from leaf explants of Gerbera jamesonii

Micropropagation of Asparagus densiflorus Jesson cv. Myriocladus