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Background. The mineral status in hypertensive patients may be affected by hypotensive drugs. The aim of this study was to estimate the influence of hypotensive drugs (angiotensine converting enzyme inhibitors (ACE-I), P-blockers, Ca-antagonists, diuretics) on the potential bioavailability of magnesium, iron, zinc and copper from buckwheat groats in vitro enzymatic digestion. Material and methods. The degree of release of magnesium, iron, zinc and copper from buckwheat groats was determined with and without (the control sample) an addition of hypotensive drugs. Four antihypertensive drugs in one dose (one tablet per sample) were analysed: metocard (a (3-blocker), cardilopin (a Ca-antagonist), apo-perindox (ACE-I) and indapen (a diuretic). The samples were subjected to enzymatic digestion under in vitro conditions. The content of minerals in buckwheat groats before and after enzymatic digestion was determined by flame atomic absorption spectrometry (AAS). Results. It was found that cardilopin (amlodipine) and indapen (indapamide) significantly increased the release of zinc from groats. The degree of release of magnesium was higher and the release of iron was lower in samples with apo-perindox (perindopril) than in the control group. The release of copper was significantly decreased by indapen (indapamid). Conclusions. Amlodipine, perindopril and indapamide affected the release of magnesium, iron, zinc and copper from buckwheat groats in vitro enzymatic digestion.
Effect of chemical composition of wheat starches applied for glucose production on the susceptibility of lipid-amylose complexes to enzymatic digestion was studied. Influence of various types of enzymatic preparations (Termamyl S α-amylase, AMG E glucoamylase, G-zyme lysophospholipase) and temperature of hydrolysis were tested. Enzymatic starch degradation was carried out in the differential MICRO DSC III scanning calorimeter (Setaram, France) within the temperature range of 20-120°C and at the scanning rate of 1°C per minute. The scanning profile was as follows: 20-105°C, 105-105°C (5 min), 105-95°C, 95-95°C (60 min), 95-60°C, 60-60°C (48 hrs), 60-40°C, 40-120°C (I heating), 120-40°C (I cooling). The enthalpy of decomposition of lipid-amylose complexes and the filtration rate of resulting glucose syrups were taken as the measure of efficiency of wheat starches liquefaction with the α-amylase and their further hydrolysis to glucose by means of glucoamylase and lysophospholipase. Chemical composition of enzymatically digested wheat starches had an impact on the degree of the lipid-amylose complexes decomposition, and physicochemical properties of starch hydrolysates. Though one of the wheat starch preparations contained more lipids, the filtration rate of its hydrolysates exceeded that of hydrolysates produced from the starch displaying lower lipid content. It probably resulted from the higher susceptibility of the lipid-amylose complexes present in this starch, to the enzymatic degradation. DSC data suggested the possibility of dependence of pathways of the complexes decomposition on polymorphs of those complexes.
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