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A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation

100%

Makowski D., Rybczynski J. J., Mikula A.

Acta Societatis Botanicorum Poloniae

2015

tom 84

nr 3

Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.

Encapsulation-dehydration technique for chrysanthemum “Lady Orange” cryopreservation

84%

Kulus D., Rewers M., Mikula A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2015

tom 96

nr 1

Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro

67%

Pawlowska B.

Folia Horticulturae

2008

tom 20

nr 1

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Folia Horticulturae

2 Mikula A.

1 Kulus D.

1 Makowski D.

1 Pawlowska B.

1 Rewers M.

1 Rybczynski J. J.

2 2015

1 2008

Wyniki wyszukiwania

A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation

Encapsulation-dehydration technique for chrysanthemum “Lady Orange” cryopreservation

Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro