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The object of the experiment was to determine the extent to which selected electrolytes (Na, K, Ca, P) and metabolites (cholesterol, total lipids, and total proteins) changed in the blood serum during the postpartum period in ewes and their correlation to litter size. Blood was collected from the jugularis v. of 149 Slovak merino breed ewes on 1, 4, 7, 14, 17, 21, 25 and 34 postpartum days. Lower Na and K levels were recorded in ewes with twins compared to ewes with a single lamb, with the exception of day 34.When evaluating Ca levels, it was found that the group of ewes with a single lamb exhibited a range from 2.38 ± 0.16 to 2.54 ± 0.26 mmol.l-1 and the group with twins a range from 2.39 ± 0.15 to 2.51 ± 0.19 mmol.l-1 and there was no significant difference. Phosphorus levels in the group of ewes with twins were lower on all observed days than those in the group of ewes with single lambs. Lower cholesterol levels were observed from day 7 of the observed period in ewes with twins compared to ewes with a single lamb. By day 21 of the observed period total lipids exhibited lower values in ewes with twins and the total serum protein levels in ewes with single lambs tended to slightly rise during this time. From day 14 postpartum there was a mild decrease in the total protein levels in the group of ewes with twins. The discussion focused on the effect of litter size on selected biochemical parameters during the postpartum period.
In pot experiments conducted on cucumber cv. Śremski F1, the effect was studied of short-term chilling stress on plants which had grown from seeds germinating in the solution of Asahi SL or treated with this biostimulator during the early growth period. The plants were grown in a phytotron at an air temperature of 27/22ºC (day/night), using fluorescent light with FAR flux density of 220 μmol x m-2 x s-1 and with a photoperiod of 16/8. The biostimulator was applied using the following methods: a) germination of seeds in 0.01% and 0.05% solution, b) watering of plants twice with 0.01% or 0.05% solution, c) spraying leaves with 0.3% or 0.5% solution. Plants sprayed with distilled water were the control. After 24 hours from foliar or root application of Asahi SL, one half of the plants from each experimental series was treated for a period of 3 days at a temperature of 12/6ºC, with all the other growth conditions unchanged. The obtained results show that short-term chilling stress caused a significant increase in electrolyte leakage, free proline content and in the activity of ascorbate peroxidase in leaves, but a decrease in the content of chlorophyll, its maximum fluorescence (Fm) and quantum yield (Fv/Fm), carotenoid content, stomatal conductance, transpiration, photosynthesis, leaf biomass and in the activity of catalase in leaves. Foliar or root application of Asahi SL in the pre-stress period decreased the values of the traits which increased as a result of chilling or increased those which decreased. Higher concentrations of the biostimulator solutions, applied using this method, were more effective. The application of the biostimulator during seed germination did not result in significant changes in the response of plants to chilling stress.
W badaniach doświadczalnych na zwierzętach laboratoryjnych określono wpływ leczniczych wód mineralnych - o różnym stopniu mineralizacji ogólnej i składzie chemicznym - zastosowanych w formie kuracji pitnych na poziom sodu, potasu, magnezu i wapnia w surowicy krwi szczurów oraz działanie tych wód na motorykę jelita cienkiego królika w warunkach in vitro.
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