The usefulness of human erythrocytes taken from donors of different blood groups was assessed in respect to their capacity to reat with a suspension of rabbit organs derived from infected animals with RHDV. According to the authors HA test should be carried out in a 0.85 per cent NaCl solution (pH = 7.0) at room temperature and the results should be read after 2—3 hours.
The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concem: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (≤30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction.