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The genetic integrity of four accessions of the cross-pollinating species rye (Secale cereale L.) was investigated. Seeds available from the first and most recent regeneration cycles, multiplied 8, 12 (twice) or 14 times were fingerprinted using microsatellite markers. In all four accessions the allele numbers and frequencies changed after regeneration. Alleles present in the original seed sample were not detectable in the regenerated populations, whereas on the other hand, alleles were found in the recent seed sample, which were not observed in the investigated plants of the original one.
Artificial hybridization experiments revealed a relatively high degree of crossability between Pinus sylvestris and P. mugo, ranging between 0.15 and 0.98. Differences between P. sylvestris x P. mugo and reciprocal hybrids of the species were observed. Hybrid seeds of P. sylvestris x P. mugo shared higher germination potential than seeds from selling, controlled intraspecific crossing, and open pollination. The corresponding value in reciprocal combination was slightly lower. The hybrid nature of the seeds obtained was shown by restriction analysis of chloroplast DNA (cp DNA) using the trnV-trnH/Hinf I primer-enzyme combination.
In Solanum muricatum Aiton the general flower structure was typical for Solanaceae. The anther wall comprised the epidermis, endothecium (restricted to the anther tip), 3-4 middle layers and secretory tapetum. Placentoids developed in the anther loculi. Tapetum degeneration was noted in buds with the corolla shorter than the calyx, while loculi were filled with microspore tetrads. At the next stage (corolla even with calyx) pollen grains were visible. The anthers opened with tip pores in the still-closed buds, and then at anthesis the stomium split along the hypodermal row of idioblasts. Inhibition of pollen tube growth in vivo was not observed under self- or cross-pollination.
Allogamy in chasmogamous flowers of V. odorata (2n = 20) was detected using classical embryology methods and crossing experiments. 48 chasmogamous flowers left to open pollination were analysed. Out of 372 ovules normal, 8-nucleate ESs were observed in 52%; in 40% of ovules zygotes or embryos with endosperm occurred; 8% of ovules studied contained ESs in earlier stages of development. Additionally in several ovules some abnormalities concerning ES structure ahd the origin of embryos were detected. Isolation of 20 chasmogamous flower buds proved that self-fertilization did not occur without the pollen vector. Cross-pollination experiments involving both intra- and inter-population pollination showed that chasmogamous flowers yealded viable seeds after cross-fertilization. Seed germination occurs only after scarification. Scarified seeds germinated under in vitro culture conditions and developed into seedlings. Genetical structure of V. odorata populations depends on many factors discussed in this paper.
Gasteria has ovular incompatibility, and recognition of cross- and self-pollen takes place. Cross-pollination includes recognition and pistil activation and leads to seed set. Self-pollen germinate, and their pollen tubes penetrate the ovules but after fertilization they abort. A group of glycoproteins in the pollen coat seems implicated as signal molecules for recognition and activation. Pistil activation is expressed as extra water uptake in the pistil, especially in the fluid pollen tube pathway, and results in higher in vitro pollen tube penetration in the ovular micropyle. In the fluid pollen tube pathway of unpollinated styles a high concentration of sucrose, glucose and fructose is present. The level of these carbohydrates remains the same during the pollen tube growth of cross-pollen. This level decreases after the passage of self-pollen through the stylar channel, and the level of carbohydrates is restored. This implies extra carbohydrate influx in the pollen tube pathway after cross-pollination. Recognition and activation signals act together after cross-pollination of Gasteria. After self-pollination the utilization of carbohydrates lowers the pistil’s carbohydrate capacity, perhaps also leading to a late-acting incompatibility.
Anther and somatic tissue culture in combination with mutagenesis were carried out to evaluate the efficiency of different mutagenic treatments of various in vitro culture materials, and to obtain some promising variants for rice improvement. Results indicated that in japonica rice radiation treatment of dry seeds and young panicles influenced the percentage of green plantlets regeneration from anther culture. Both treatments increased significantly the percentage of regenerated green plantlets in comparison with the control. Irradiation with 30 Gy of rice callus increased also the percentage of regenerated green plantlets. For indica rice, the combination of the suitable dose of gamma rays irradiation on seeds and an improved medium, increased the percentage of callus induction. This approach made it possible to use anther culture in indica rice breeding. Somatic tissue cultures combined with radiation-induced mutagenesis led to the development of a number of promising mutants including some new cytoplasm-nucleus interacting male-sterile lines with almost 100% stigma exsertion. Their development would be of practical significance for increasing the genetic diversity for production of hybrid rice.
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