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The aim of the study was to compare the effectiveness of hormonal stimulation of the female ide (Leuciscus idus L.), following the application of two commercial preparations concerning different GnRHa combined with dopamine inhibitors: Ovopel and Ovaprim. Ide spawners were bred under controlled conditions. The examined parameters included the percentage of ovulating females, time of ovulation and the rate of embryo survival to the eyed-egg stage. The growth and survival rates for larvae produced by farm and wild fish were compared. Stimulation with Ovopel resulted in a shorter latency time (36), whereas stimulation with Ovaprim resulted in a higher percentage of live embryos at the eyed-egg stage (79.7 %). Eggs was obtained from 20% of females in the control group, whereas in treated groups it was at level 90–100%. No differences were found between the growth rate of the ide larvae produced by the farm fish and that obtained from wild fish.
The sweet potato whitefly, Bemisia tabaci (Genn.) (Biotype A: Hem.: Aleyrodidae) is one of the most important pests in Iran. The development of alternative methods that are not chemical applications, is necessary in pest management for the sake of human health and for the safety of the environment. In this research project, the effect of four herbal compounds Fumaria parviflora Lam. (Fumariaceae), Teucrium polium L. (Lamiaceae), Calotropis procera (Willd.) R. Br. (Asclepiadaceae), Thymus vulgaris L. (Lamiaceae) on the same biological parameters of cotton whitefly were compared with the insecticides azadirachtin and pymetrozin. The results showed significant differences between herbal compounds and insecticides on: egg longevity (F6, 39 = 2.96, p < 0.05), pupa longevity (F6, 37 = 4.49, p < 0.01) and adult longevity (F6, 31 = 4.47, p < 0.01); egg mortality (F6, 39 = 6.71, p < 0.001), total egg mortality (F6, 39 = 6.71, p < 0.001), sex ratio (female) (F6, 30 = 3.49, p < 0.01), oviposition period (F6,31 = 7.50, p < 0.001) and total fecundity/female (F6, 31 = 8.23, p < 0.001). These results indicated that a fumitory extract and pymetrozin had the best effect on longevity and mortality, respectively. According to the results, the fumitory had a noticeable effect on the different life stages of the sweet potato whitefly.
Microbiological characteristics of sewage sludge from a mechanical-and-biological sewage treatment plant composted in controlled conditions with straw and sawdust are presented. Prepared composts were placed in four bioreactors with airflow of 4 l air·min⁻¹. In bioreactor K1, K2 and K3 the composted mass consisted of 65% sewage sludge (K1–sewage sludge 1, K2 – sludge 2, K3 – sludge 3) + 30% sawdust + 5% straw; while in bioreactor K4 the proportion was: 45% sludge 2 + 50% sawdust + 5% straw. Compost samples were taken from all chambers at the same time, depending on actual temperature. Microbiological analyses consisted in the determination (by plate method) on selective media of the numbers of mesophilic and thermophilic bacteria, fungi and pathogenic bacteria Salmonella sp. Clostridium perfringens and Enterobacteriaceae. Furthermore, in the experiment, the activity levels of dehydrogenase were determined using 1% triphenyltetrazole chloride as substratum. Studies have indicated that the composting process caused a decrease of the number of fungi and pathogenic bacteria from Enterobacteriaceae family and Clostridium perfringens in all composted matters, as well as an increase in the number of thermophilic bacteria. Changes in the number of mesophilic bacteria depended on the compost type. In composts K1 and K2, the composting process caused an increased proliferation of cells, while in the composts K3 and K4 the number of mesophilic bacteria decreased. On the basis of the obtained results, it was also found that in the majority of analysis terms, the lowest activity of dehydrogenases occurred in compost K3, while their level of its activity, in the majority of the studied composts, correlated most intensively with the number of thermophilic bacteria.
In this study the suitability of various feeds in mass rearing of burbot Lota lota (L.) larvae under controlled conditions was determined. During the rearing the fish were fed with: artificial fodder, live Artemia nauplii and decapsulated cysts of artemia. The experiment continued for 20 days and it was conducted from hatching of the larvae. The initial density of fish during rearing was 1000 individuals dm⁻³. Rearing water temperature was set at 12℃. The parameters such as: average body length and height of the larvae and survival after completion of rearing period, were recorded. The Artemia nauplii proved the most suitable for feeding burbot larvae during the initial rearing. Additionally, high mortality of larvae was recorded during the period of filling the swim bladder.
Juvenile of Trichogaster lalius was reared under three photoperiod conditions (0L:24D, 12L:12D, 16L:08D) for 60 days to examine the growth performance under laboratory conditions. The maximum growth was observed in the juvenile of T. lalius exposed to 16:00 hrs. light. The mean body weight of different groups of juvenile fish exposed to different light conditions were significantly different (p<0.05) from each other which was observed from 20thday of the experiment. The absolute, specific, and relative growth rates were found to be maximum in the group exposed to 16:00 hrs. light duration. The present study indicated that 16:00 hrs. light duration was considered to be better for the growth of juvenile of T. lalius under controlled condition.
The growth and survival of burbot Lota lota (L.) larvae reared from the first feeding on freshly hatched Artemia franciscana nauplii at 12, 15, 18, 21, and 24ºC was compared in a 20-day experiment. Nauplii of the commercial strain Artemia proved to be an excellent start diet for L. lota larvae. The best fish growth was found at 21ºC where they attained a final average total length of 14.4 mm and an average body weight of 37.1 mg. The highest final survival rates of 90 and 92% were recorded at 12 and 15ºC, respectively. At all higher water temperatures, larval final survival rates were significantly lower, with the minimum value of 41% at 24ºC. In light of these results, it is evident that either temperatures lower or higher than 21ºC are outside of the optimal range for L. lota larvae as they produce relatively slow growth or poor survival or both.
Artificial reproduction of asp under controlled conditions was done using two different spawning agents based on GnRH analogues and dopamine antagonists (Ovopel and Ovaprim). Fish in the Ovopel and Ovaprim and combined treatment groups were treated with a dose equivalent to 1.2 pellets (0.2 and 1.0), 0.5 cm³ liquid (0.1 and 0.4) and 0.2 pellets and 0.4 cm³ liquid per kg of body weight respectively. The highest percentage of ovulation (100%) and embryo-survival to the eyed-egg-stage (81.3%) was recorded after the application of a combination of Ovopel and Ovaprim in comparison with other groups. Fish from the control group did not ovulate. The latency time was shorter in the groups where Ovopel and Ovopel with Ovaprim was applied (40) than in Ovaprim group (42–44 hrs). The obtained results indicates that combination of Ovopel with Ovaprim might be successfully used for artificial reproduction of asp.
This study determined the effect of hormonal stimulation of the wild female nase, Chondrostoma nasus (L.), on its basic reproduction indices (percentage of ovulations, latency time, embryo survival) and the economic profitability of its use. Two commercial preparations were used in the experiment: Ovopel and Ovaprim. They were used separately (group 1 and 2 for Ovopel and Ovaprim, respectively) and in combination (group 3), where Ovopel was given in initial and Ovaprim in resolving injection. The study found a high effectiveness of all the hormonal treatments applied (ovulation rate 90–100%, latency time 36 h, embryo survival rate 78.6–81.2%) (P>0.05), which may be evidence of the greater susceptibility of the nase to stimulation with the less active mammalian analogue of GnRH as compared to other species of rheophilic cyprinids. In consequence, the lowest cost of hormonal stimulation (0.59 EUR per 10,000 viable embryos) was achieved with Ovopel. Using the hormonal agents in combination (in group 3) reduced the cost of stimulation by 0.17 EUR as compared to Ovaprim (group 2), where the cost was the highest (1.57 EUR per 10,000 viable embryos). The results presented in this study are providing useful information for fish breeders who manage wild populations of the nase and other species of rheophilic cyprinids.
Based on a three-year field experiment under controlled condition in Ji’nan, China, the effects of peanut growth on the variation in the abundance and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA) before and after peanut growth were investigated through quantitative PCR and cluster analysis of terminal-restriction fragment length polymorphism. Our results show that the community composition of AOA and AOB was greatly affected by the peanut growth leading to the decreased abundance of AOA and increased abundance of AOB. Furthermore, AOA and AOB community structures varied before and after peanut growth. Phylogenetic analysis indicated that all AOA and AOB community sequences were clustered into the uncultured group. Altogether, the results suggested that the abundance of AOA and AOB in soil and their community compositions can be greatly affected by the peanut growth.
The growth, size variability, condition coefficient and percentage of fish with external deformities were compared in juvenile crucian carp (initially 31 mm TL and 0.36 g BW) which were fed intensively four starters only for 120 days at 25ºC. The best weight gain was achieved by fish fed an experimental carp diet with the lowest fat content (7.4%) and a commercial diet for eel containing 15.9% fat (4.33 and 4.15 g BW, respectively). In contrast to the experimental diets, both commercial diets produced fish with high condition coefficient values and a very high (37.2 or 62.7%) percentage of individuals with different external deformities. Size variability did increase over the course of the experiment except in the fish fed the high-energy diet for eel. Survival rates ranged between 96.5 and 99.5%. Our results indicate that juvenile crucian carp can be successfully reared exclusively on dry diets but not the commercial ones for eel or carp used in the present experiment.
Growth, survival, condition (Fulton's coefficient) and biological quality (salinity stress test) were compared in 2-month-old burbot (20 mg BW, 10.9 mm TL) reared at 12,15,18, and 21°C. At 15°C, the fish showed the best growth in BW and TL (170 mg and 24.5 mm, respectively), and the highest final survival rate (97%), whereas their condition coefficient and tolerance to salinity proved to be the second best. This may indicate that a temperature of 15°C should be used for the practical purposes of juvenile burbot mass production under controlled conditions.
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