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The number, topographic sequence and duration of the moults of the coat of Mus musculus Linnaeus, 1758 are analysed using wild specimens from collections and albino specimens born and kept in the laboratory. Mice undergo an uninterrupted series of regular moults throughout its life. Each has a typical duration. The juvenile moult is the fastest and in all cases the speed of moulting is unconstant. At first the rate is accelerated but it frequently decelerates, and even pauses, so that some parts of the body, mainly the cephalic area, do not moult. This pause leads to a superposition of waves of different moults with the coexistence of 3 or more coats. The superpositions, revealed by dying the laboratory animals' coats, could be the reason why previous authors have claimed the existence of irregular moults in M. musculus. With the wild specimens, despite the fact that the inner surface of the skin yields less information, moulting patterns, similar to those described in the laboratory specimens, may be detected.
The aim of the study was to determine the content and biological activity of phenolic compounds of three faba bean genotypes - a coloured flowered Nadwiślański variety, a coloured flowered "self-ending" Tinos variety and white flowered Caspar variety. Comparing the white flowered Caspar variety containing 0.34% of phenolic compounds in the seed coat, in this proantho- cyanidins made from 0.04%, the Polish Nadwiślański and Tinos variety were characterised as having a higher phenolic compound content in the seed coat, i.e. 8.70% and 11.75%, respectively. The proanthocyanidins content, being 6.54% and 8.32% respectively, is in accordance with the lower values determined in the seed coats of many European high-tannin faba bean varieties. Seed coats containing a higher level of polyphenols, added to casein diets in amounts of 15%, highly significantly lowered the digestibility and protein efficiency. Depending on the variety of seed coat, TD protein coefficients amounted to: Caspar — 85.4%, Nadwiślański — 71.3%, Tinos — 69.3%. For these same diets the NPU index was 71.2, 59.3 and 49.4, and the PER index was 2.63, 2.23 and 2.16, respectively. It was also found highly significant decrease of Ca, Fe, and Zn apparent digestibilities in casein diets with faba bean seed coats of a high polyphenolic content.
The aim of the study was to determine the effect of an organic form of mineral supplement containing copper, zinc and manganese on the concentration of these elements in the coat (hair) of Polish Konik horses. Possible impact of the rearing conditions of 24 Polish Konik foals (which originated from stables and nature reserves), their sex and duration of the supplementation on the coat’s mineral composition was considered. The research included 24 horses: 12 stabled (six fillies and six colts) and 12 captured in a nature reserve (seven fillies and five colts). Ten samples of fodder and 60 samples of coats were analysed. The influence of the rearing system on the concentration of elements in the coat was observed, for example stabled horses had a higher content of copper in the coat while the content of zinc and especially manganese was lower. All horses, both reared in a stable and from the nature reserve, showed higher copper concentrations in their coat (P ≤ 0.05) after supplementation with copper, while an increased amount of zinc was observed only in animals reared in a stable. All horses (both stabled and wild ones) demonstrated an evident change in the manganese content of their coat after supplementation with a chelate. The content grew significantly (P ≤ 0.01) with the supplementation time. An interaction between the sex and chelate supplementation time was observed only in the case of manganese administered to animals reared in a stable.
Effect of the brown coat-coding gene (TYRP-1) on wool and skin color of Żelaźnieńska and Wrzosówka sheep. The study was conducted on randomly chosen ewes (Żelaźnieńska sheep – 93; Wrzosówka sheep - 133) during the shearing. Color of wool and skin was examined using device Chroma Mater CR-400 (Konica Minolta Sensing Inc., 2011). Taking into account the results of genotyping in order to the brown coat-coding gene (TYRP-1), 66 Żelaźnieska ewes and 74 Wrzosówka ewes were chosen to next stage of study where effect of the brown coat-coding gene (TYRP-1) on wool and skin color was assessed within breed and between breeds. Based on the results significant and highly significant differences in all color measurements of wool and skin between tested breeds were found, which should be connected with different wool color in each breed. However, there is striking difference in color of wool and skin regarding to a* color parameter, which were exactly opposite. It probably means that proportion of red or green color in skin is different than in wool. Differences in color values of wool depending on TYRP-1 gene genotypes were observed only for Wrzosówka sheep. The measurement of L* color parameter made on wool was highly significantly higher in the case of CC and CT genotypes in comparison to TT genotype. However, in the measurement of a* color parameter, the situation is opposite and homozygote TT had higher values compared to the others genotypes. No differences between wool and skin color of Żelaźnieńska sheep and no differences in skin color of Wrzosówka sheep were found. The results of studies on wool color, depending on genotype of the TYRP-1 gene in Wrzosówka sheep, make possibilities to conduct breeding work in order to develop standards for coat color for this breed.
The investigations aimed at the assessment of nickel contamination of the environment based on the content of this element in the coat of domestic and feral cats. The content of nickel in the coat was determined using the method ICP-OES. While assessing the state of nickel supply, a trial was undertaken at checking whether the increase in nickel content in the animal organism affects biological and haematological indexes of the animal. Blood plasma alkaline phosphatase, and alanine and aspartate aminotransferase (transaminase) activities, and haematological parameters were analysed. Then, the correlations between those indexes and the nickel content in cats' hair depending on cats' keeping (feral and domestic cats) and their sex were calculated. No statistically significant differences were noted depending on the cats living conditions. The obtained mean value of nickel content in the coat could be accepted as normal for cats. In the case of nickel, the differences depending on sex and age were also not significant statistically. The assessment of statistical indexes shows that median, similarly as lower quartile, are identical for the nickel content in the cats from both groups (0.40 mg·kg⁻¹) independently from their sex and age. Analysis of nickel content depending on hair colour was also performed assuming that the colour depends on the saturation of hair with melanin. The lowest nickel content was observed in white hair, higher in tortoiseshell hair and black hair, and the highest in the feral colour - brownish grey. The results confirmed the fact that the content of nickel depends on the hair saturation with melanin.
Basal metabolic rate (BMR) of the European pine vole Pitymys subterraneus (de Sélys-Longchamps, 1836) with typical black agouti coat colour (A) is 2.78 cc O2 g"1 hr" , and in voles with diluted fur pigment (D) it is 3.06 cc O2 g"1 hr"1. The difference of BMR between the voles of A and D morphs is not significant. Resting metabolic rate (RMR) was determined at ambient temperatures (Ta) -10°C, 0°C, 6°C, 12°C, 24°C and 30°C. P. subterraneus of A morph decrease RMR from 9.21 (at -10°C) to 4.80 cc O2 g"1 hr 1 (at 30°C) while the voles of D morph decrease RMR from 10.43 to 4.01 cc O2 g"1 hr'1, respectively. The differences of RMR between the voles of A and D morphs are significant at Ta -10°C, 0°C, 6°C, 24°C, and 30°C. Maximum oxygen consumption rate (VO2NA), after injection of noradrenaline, is not dependent on Ta (measurements were made at -10°C, 0°C, 12°C and 24°C). It equals 8.96 cc 02 g"1 hr"1 in P. sub­terraneus of A morph and 10.18 cc O2 g"1 hr"1 in the voles of D morph, and the difference is significant.
The HPLC analysis of polyphenolic compounds extracted from seed coats of coloured and white beans as well as definition of antioxidant properties of the obtained extracts constituted the purpose of the hereof elaboration. The materials for the research were obtained by extraction of the polyphenols with 0.5% HCl solution in methanol, vacuum concentration and lyophilisation. In the research, defractioning of particular polyphenols occurred with the use of solid-phase extraction and thiolysis methods. Polyphenols were identified by high performance liquid chromatography (HPLC). The antioxidant properties of polyphenolic preparations were determined by fluorometric method. Hydroxide radicals were generated from hydrogen peroxide and sodium benzoate in the presence of copper ions and ditioerythritol (DTET). It was stated that the extracts of polyphenols obtaned from seeds of coloured and white beans indicate antioxidant properties against hydroxide radicals.
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