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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Investigation of biofilm formation ability of coagulase-negative staphylococci isolated from ready-to-eat meat

100%
Woroszylo M., Pendrak K., Ciecholewska D., Padzik N., Szewczuk M., Karakulska J.
Acta Scientiarum Polonorum. Zootechnica
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2018
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tom 17
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nr 4
2
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Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

75%
Bochniarz M., Wawron W.
Polish Journal of Veterinary Sciences
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2012
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tom 15
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nr 1
The aim of the present study was to assess the haemolytic and proteolytic activity of coagulase- negative staphylococci (CNS) isolated from cows with mastitis. The study was conducted on 100 CNS strains: S. xylosus (n=28), S.chromogenes (n=26), S.haemolyticus (n=25), S. sciuri (n=14), S. warneri (n=4), S.hominis (n=2), S.saprophyticus (n=1); 22 CNS were isolated from cows with clinical mastitis and 78 from those with subclinical mastitis. The CNS studied showed the ability to produce only α-haemolysin and belonged to one strain – S. haemolyticus (21.0% of isolated CNS strains). Haemolysin-positive CNS were responsible for both clinical and subclinical mastitis (22.7% and 20.5%, respectively). The ability to produce protease was found in 31.0% of CNS belonging to two strains: S. chromogenes and S. sciuri. Protease-positive CNS were the etiological factor of both clinical and subclinical mastitis (31.8% and 30.8%, respectively). All S. xylosus, S. warneri, S. hominis, and S. saprophyticus strains were found protease-negative and haemolysin-negative, irrespective of whether they caused clinical or subclinical mastitis in cows.
3

Current concepts on the impact of coagulase-negative staphylococci causing bovine mastitis as a threat to human and animal health - a review

63%
Sender G., Pawlik A., Korwin-Kossakowska A.
Animal Science Papers and Reports
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2017
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tom 35
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nr 2
Staphylococci are the main aetiological factor of bovine mastitis in many dairy herds. Traditionally, coagulase-negative staphylococci (CNS) were considered a normal skin microbiota. Lately the role of CNS in bovine mastitis has increased, as in some countries these pathogens had started to outnumber other mastitis aetiological factors. Given the scale of mastitis problems, the intensive use of antibiotics in dairy cattle, the number of animals and the consumption of milk products there is an urgent need to highlight the threat to both human and animal health originating from CNS. The knowledge of how CNS mastitis develops, spreads in herd and persists is limited. Bovine strains of CNS are poorly characterised; moreover, they are characterised mainly in terms of phenotypes. This review summarises knowledge on the characterisation of CNS strains, stressing the role of the bacterial genotype, in the context of the risk to human and animal health. Selected CNS virulence factors that play a role during mastitis in dairy cattle are listed and described in this paper. They have been selected subjectively by the authors in view of their significance for public health (toxins, antimicrobial resistance) and their importance for animal health (formation of biofilm, prevention of phagocytosis intracellular survival) and mastitis outcome.
4

Identyfication of Staphylococcus spp. strains isolated from milk samples of mastitic cows by PCR-RFLP analysis of gap gene

63%
Karakulska J., Sawicka I.
Advances in Agricultural Sciences
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2008
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tom 12
5

Detection of methicillin resistance in hospital environmental strains of coagulase-negative staphylococci

63%
Nowak T., Balcerczak E., Mirowski M., Szewczyk E. M.
Polish Journal of Microbiology
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2006
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tom 55
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nr 4
The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulase-negative staphylococci (CoNS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX₃₀) and oxacillin (OX₁), oxacillin agar screen test with 6 mg/l of oxacillin (MHOXA), latex test for PBP2a (LA) and detection of mecA via PCR. One hundred and one isolates were methicillin-resistant in at least one of methods used, but only 74 were mecA -positive. Of the 68 mecX-negative strains: two were positive by OX₁, the LA and MHOXA methods; three by the LA and MHOXA; and 22 only by OX₁, test. Most of these strains were from the novobiocin-resistant CoNS group. The results obtained for all tested strains using FOX₃₀ showed complete concordance with the presence of the mecA gene.
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