Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Ascorbate and photosynthesis: how does Arabidopsis adjust leaf ascorbate concentration to light intensity?

100%

Smirnoff N., Page M., Ishikawa T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 2

2

The relationship between mitochondria function and circadian clock in peripheral oscillators

100%

Kazmierczak A., Schmitt K., Grimm A., Strosznajder J. B., Eckert A.

Acta Neurobiologiae Experimentalis

|

2013

|

tom 73

|

nr 1

Circadian rhythms govern a wide variety of physical, behavioral and metabolic changes that follow a roughly 24-hour cycle, responding primarily to light and darkness in an organism’s environment. These are controlled by the circadian clock mechanism, where rhythm-generating mechanism is encoded by a transcription-translation feedback loop. Numerous studies have pointed to a cyclic relationship wherein the rhythm impacts metabolic activity and metabolism feeds back to impinge upon the rhythm. Mitochondria play a pivotal role in regulating cellular energy and were shown to be strategically positioned at the intersection between circadian rhythm and cell metabolism. Nevertheless little is known about their function in controlling the circadian rhythm. In our study, we investigated the involvement of circadian clock in mitochondrial function as well as mitochondria-dependent regulation of circadian clock. The study was carried out in primary human fibroblasts, an already established model to investigate molecular clock mechanisms in vitro. We have found that mitochondria activity as well as network activities showed rhythmic changes within 24 hours. Circadian pattern was detected for mitochondrial ROS including superoxide anion production. A significant 24-hour oscillation was found for cellular redox state. Furthermore, mitochondrial ATP levels were rhythmic and the maximum of ATP production paralleled the peak of mitochondrial ROS level and the mitochondrial network formation. Circadian rhythm was also detected for calcium ions concentration. Increase of ATP synthesis as well as changes in calcium and ROS level activated AMP-dependent protein kinase (AMPK). We have found that in primary human fibroblasts AMPK protein level and activity fluctuate in an antiphase relationship with rhythmic ATP production. Summarizing, our data provide the evidence for circadian regulation of mitochondrial dynamics and suggest that changes of mitochondrial activity may directly influence cellular clock. Supported by grants from Sciex 10. 258 to A.K. as well as Swiss National foundation (SNF No 310030_122572) and Synapsis Foundation to A.E

3

Biochemical and structural studies of plant circadian clock proteins

80%

Saini R., Szpotkowski K., Kozak M., Jaskolski M., Davis S. J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 1

4

Neurotransmitters and their receptors in circadian clock circuits of Drosophila

80%

Nassel D. R., Enell L. E., Hamasaka Y., Johard H. A. D.

Pestycydy

|

2005

|

nr 3

133-140

5

The light/dark cycle disruption affects hepatic function both in metabolic parameters and tissue structure in a nocturnal desert rodent: Gerbillus tarabuli

80%

Rouibate A. D., Banhafri N., Ouali-Hassenaoui S., Dekar-Madoui A.

Folia Histochemica et Cytobiologica

|

2020

|

tom 58

|

nr 3

6

Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock

80%

Kotwica J., Ciuk M. A., Joachimiak E., Rowinski S., Cymborowski B., Bebas P.

Journal of Physiology and Pharmacology. Supplement

|

2006

|

tom 57

|

nr 8

107-123

The male reproductive tract of Lepidoptera is an ideal model for the study of the physiological role of peripheral clocks in insects. The latter are significant in the generation and coordination of rhythmic phenomena which facilitate the initial stages of sperm capacitation. This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase. Our aim was to determine the potential involvement of carbonic anhydrase (CA) in this process, an enzyme tasked with generating protons subsequently utilized by H+-ATPase to acidify the UVD milieu in S. littoralis, during the time when the lumen of this organ is filled with sperm. We attempted to answer the question whether CA activity can be controlled by the biological oscillator present in the male reproductive tract of the cotton leafworm. Using PAGE zymography, the presence of CA was demonstrated in the UVD wall, but not in the luminal fluid nor in the sperm. Using histochemistry, it was shown that CA is active in the UVD epithelium, and that this activity varies throughout the day and is most likely controlled by an endogenous biological clock. Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.

7

The role of dopamine in the regulation of melatonin biosynthesis in vertebrate retina

80%

Zawilska J. B.

Acta Neurobiologiae Experimentalis

|

1994

|

tom 54

|

nr S

8

Changes of ZTL protein level in Pharbitis nil seedlings exposed to varoius light conditions

80%

Zienkiewicz A., Szmidt-Jaworska A., Kopcewicz J.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

9

The effects of lesions of the thalamic intergeniculate leaflet on the circadian locomotor activity rhythm after light and dark pulses in mice

80%

Gaweda M.

Acta Biologica Cracoviensia. Series Zoologia

|

2001

|

tom 43

Ograniczanie wyników

Ascorbate and photosynthesis: how does Arabidopsis adjust leaf ascorbate concentration to light intensity?

The relationship between mitochondria function and circadian clock in peripheral oscillators

Biochemical and structural studies of plant circadian clock proteins

Neurotransmitters and their receptors in circadian clock circuits of Drosophila

The light/dark cycle disruption affects hepatic function both in metabolic parameters and tissue structure in a nocturnal desert rodent: Gerbillus tarabuli

Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock

The role of dopamine in the regulation of melatonin biosynthesis in vertebrate retina

Changes of ZTL protein level in Pharbitis nil seedlings exposed to varoius light conditions

The effects of lesions of the thalamic intergeniculate leaflet on the circadian locomotor activity rhythm after light and dark pulses in mice