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1

A novel approach to study the effect of ciprofloxacin on biofilms of Corynebacterium spp. using confocal laser scanning microscopy

100%
Olender A., Bogut A., Magrys A., Krol-Turminska K.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 4
Non-diphtherial corynebacteria are Gram-positive rods that cause opportunistic infections, what is supported by their ability to produce biofilm on artificial surfaces. In this study, the characteristic of the biofilm produced on vascular and urological catheters was determined using a confocal microscopy for the most frequently involved in infections diphtheroid species. They were represented by the reference strains of Corynebacterium striatum ATCC 6940 and C. amycolatum ATCC 700207. The effect of ciprofloxacin on the biofilm produced by the antibiotic-susceptible C.striatum strain was evaluated using three concentrations of the antimicrobial agent (2×, 4×, and 6× the MIC – the Minimum Inhibitory Concentration). The basis for the interpretation of results was the statistical analysis of maximum points readings from the surface comprising a total of 245 areas of the biofilm image under the confocal microscope. It was observed that ciprofloxacin at a concentration equal to 4×MIC paradoxically caused an enlargement of areas with live bacteria within the biofilm. Biofilm destruction required the application of ciprofloxacin at a concentration higher than 6×MIC. This suggests that the use of relatively low doses of antimicrobial agents may increase the number of live bacteria within the biofilm, and further facilitate their detachment from the biofilm’s structure thus leading to the spread of bacteria into the bloodstream or to the neighboring tissues. The method of biofilm analysis presented here provides the original and novel approach to the investigation of the diphtheroid biofilms and their interaction with antimicrobial agents.
2

Ciprofloxacin treatment of urinary infections results in increased resistance of urinary E. coli to ciprofloxacin and co-trimoxazole

100%
Borgmann S., Jakobiak T., Gruber H., Schroder H., Sagel U.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 4
371-373
Between 2000 and 2006 the sum of ciprofloxacin and folic acid antagonists prescriptions to Bavarian (South-eastern Germany) outpatients stayed constant. However, prescription numbers of ciprofloxacin increased while those of folic acid antagonists decreased suggesting an apparent shift in the treatment of urinary infections toward ciprofloxacin. During the observation period the proportion of E. cbti resistant against ciprofloxacin increased from 5% to 10% while that against co-trimoxazole increased from 21 % to 27%. The proportion of E. coli simultaneously exhibiting resistance to ciprofloxacin and co-trimoxazole increased from 3.9% to 8.5%. A leading influence of ciprofloxacin application for these developments is discussed.
3

In vitro influence of ciprofloxacin on selected functions of rainbow trout (Oncorhynchus mykiss) leucocytes

84%
Sieroslawska A., Siwicki A. K.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 3 Suppl.
4

Antibacterial activity of ciprofloxacin and trimethoprim, alone and in combination, against Vibrio cholerae O1 biotype E1 Tor serotype Ogawa isolates

84%
Mandal S., Pal N. K., Chowdhury I. H., Debmandal M.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 1
57-60
In this communication, the ciprofloxacin-trimethoprim (Cp-Tm) combination showed synergistic (Fractional Inhibitory Concentration, FIC index 0.399) and additive (FIC index 0.665-0.83) effects against Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates having Cp MICs 10 μg/ml and Cp 0.66 μg/ml, respectively, following agar dilution checkerboard method. The time-kill study results demonstrated synergy between Cp and Tm against both groups of isolates providing 2.04 log₁₀ (for strain with Cp MIC 0.66 μg/ml) and 3.12 log₁₀ (for strain with Cp MIC 10 μg/ml) decreases in CFU/ml between the combination and its most active compound. Thus, the findings of the present study suggest an introduction of Cp-Tm combination treatment regimen against drug resistant cholera and this in turn will help in combating the drug resistance of V. cholerae O1 biotype El Tor serotype Ogawa.
5

gyrA mutations in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa in a Silesian hospital in Poland

84%
Wydmuch Z., Skowronek-Ciolek O., Cholewa K., Mazurek U., Pacha J., Kepa M., Idzik D., Wojtyczka R. D.
Polish Journal of Microbiology
|
2005
|
tom 54
|
nr 3
Among 73 clinical isolates of Pseudomonas aeruginosa 48 strains were Ciprofloxacine (CIP) susceptible and 25 CIP resistant (Minimal inhibitory concentration - MIC>32 μg/ml - 14 strains) or of intermediate susceptibility to CIP (MIC≥ 1,5-32 μg/ml - 11 isolates). Mutations in the quinolone-resistance-determining region (QRDR) of gyrA gene were searched in groups of CIP resistant and of intermediate susceptibility to CIP isolates. Two methods: restriction fragment length polymorphism (RFLP) analysis and DNA sequencing analysis allowed to detect three different mutations. The nucleotide substitutions observed led to the following amino acid replacements: Thr-83 —> Ile, Asp-87—>Asn, Asp-87—>Gly. One mutated strain among the group of mutants analyzed showed double mutation (Thr-83—>Ile, Asp-87—>Gly) and additional silent mutation (Val-103—> Val); whilst the rest of the isolates showed different single missense mutations. The most frequently detected mutation in the gyrA gene (16 out of 25 mutants) was the Thr-83—>Ile substitution.
6

Susceptibility of Campylobacter jejuni and Compylobacter coli isolated from animals and humans to ciprofloxacin

67%
Tambur Z., Miljkovic-Selimovic B., Bokonjic D., Kulisic Z.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 2
269-273
Fifty five thermophilic Campylobacter spp. strains were isolated from the caecum of broilers, cecum and colon of pigs and from human faeces. The strains were identified as Campylobacter jejuni and Campylobacter coli. The more prevalent species in humans and broilers was Campylobacter jejuni, and in pigs Campylobacter coli. In the framework of this study, sensitivity to ciprofloxacin in isolated strains of Campylobacter jejuni and Campylobacter coli was tested by E-test. Resistant to ciprofloxacin were 50.0% of 24 thermophilic Campylobacter strains isolated from humans. In 16 tested strains isolated from broilers, 56.2% were resistant to ciprofloxacin. More resistant species was Campylobacter coli (83.3%). In 15 strains of thermophilic Campylobacter spp. isolated from pigs, the percentage of resistant strains was 26.7%, a relatively high percentage considering the quinolones have not been extensively used in swine farming compared to poultry farming.
7

Effect of ciprofloxacin and N-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces

67%
El-Feky M. A., El-Rehewy M. S., Hassan M. A., Abolella H. A., Abd El-Baky R. M., Gad G. F.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 3
261-267
The aim of this study was to evaluate the effect of ciprofloxacin (CIP), N-acetylcysteine (NAC) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. Two strains each of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klehseilla pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris, recently isolated from patients undergoing ureteral stent removal and shown to be capable of biofilm production, were used in this study. The inhibitory effects of ciprofloxacin, N-acetylcysteine and ciprofloxacin/N-acetylcysteine combination were determined by static adherence assay. Ciprofloxacin (MIC and 2 MIC) and N-acetylcysteine (2 and 4 mg/ml) inhibited biofilm production by ≥60% in all tested microorganisms. Disruption of pre-formed biofilms of all tested microorganisms was found to be ≥78% in the presence of ciprofloxacin (MIC and 2 MIC) and ≥62% in the presence of N-acetylcysteine (2 and 4 mg/ml), compared to controls. Ciprofloxacin/N-acetylcysteine showed the highest inhibitory effect on biofilm production (94-100%) and the highest disruptive effect on the pre-formed biofilms (86-100%) in comparison to controls. N-acetylcysteine was found to increase the therapeutic efficacy of ciprofloxacin by degrading the extracellular polysaccharide matrix of biofilms. These data are statistically significant. The inhibitory effects of ciprofloxacin and N-acetylcysteine on biofilm production were also verified by scanning electron microscope (SEM). In conclusion, Ciprofloxacin/N-acetylcysteine combinations have the highest inhibitory effect on biofilm production and the highest ability to eradicate pre-formed mature biofilms.
8

Opornosc na ciprofloksacyne i tetracykline szczepow Campylobacter spp. izolowanych z produktow drobiarskich

67%
Mackiw E., Rzewuska K., Tomczuk K., Korsak D.
Bromatologia i Chemia Toksykologiczna
|
2009
|
tom 42
|
nr 3
588-592
9
Content available remote

Ciprofloxacin inhibits proliferation and promotes generation of aneuploidy in Jurkat cells

67%
Koziel R., Szczepanowska J., Magalska A., Piwocka K., Duszynski J., Zablocki K.
Journal of Physiology and Pharmacology
|
2010
|
tom 61
|
nr 2
Ciprofloxacin is widely used in antimicrobial therapy. However it also inhibits mitochondrial topoisomerase II and therefore affects cellular energy metabolism. At a concentration exceeding 80 µg/ml ciprofloxacin induces apoptosis, while at 25 µg/ml it inhibits proliferation of Jurkat cells without any symptoms of cell death. The aim of this study was to explain the mechanisms of ciprofloxacin-evoked perturbations of the cell cycle. Human lymphoidal cells (Jurkat) were exposed to ciprofloxacin (25 µg/ml) for 4-11 days and effects of the drug on cell proliferation (light microscopy), cell cycle (flow cytometry), cell size and morphology (confocal microscopy) as well as number of chromosomes (chromosomal spread analysis) were investigated. Exposition of Jurkat cells to ciprofloxacin inhibited cell proliferation, increased proportion of cells in the G2/M-phase of the cell cycle, compromised formation of the mitotic spindle and induced aneuploidy. These observations indicate that ciprofloxacin applied at concentrations insufficient for induction of apoptosis may stop cell proliferation by inhibition of mitosis. Chromosomal instability of such cells may, at least potentially, increase a risk of cancer development.
10
Content available remote

A study comparing the efficacy of antimicrobial agents versus enzyme (P-gp) inducers in the treatment of 2,4,6 trinitrobenzenesulfonic acid-induced colitis in rats

67%
Toklu H. Z., Kabasakal L., Imeryuz N., Kan B., Celikel C., Cetinel S., Orun O., Yuksel M., Dulger G. A.
Journal of Physiology and Pharmacology
|
2013
|
tom 64
|
nr 4
11
Content available remote

Pleuritis as the first symptom of pulmonary embolism: a case report

67%
Doboszynska A., Koc M., Koc K., Swietlik E., Sosnowska E., Tomaszewska I.
Journal of Physiology and Pharmacology. Supplement
|
2007
|
tom 58
|
nr 5
12

Novel gyrase mutations and characterization of ciprofloxacin-resistant clinical strains of Enterococcus faecalis isolated in Poland

67%
Piekarska K., Gierczynski R., Lawrynowicz-Paciorek M., Kochman M., Jagielski M.
Polish Journal of Microbiology
|
2008
|
tom 57
|
nr 2
The activity of ciprofloxacin, sparfloxacin and moxifloxacin was determined for 205 Enterococcus faecalis isolates from patients of five hospitals (Warsaw, Poland; collected from 2000 to 2002). Ciprofloxacin resistant and intermediate isolates were numerous (53.7%). Among them, highly resistant (MIC≥16 mg/l) isolates predominated (98%). Isolates resistant to ciprofloxacin were also resistant to sparfloxacin and moxifloxacin. The parC and gyrA QRDRs (quinolone-resistance-determining region) of 11 isolates with ciprofloxacin MICs from 1 to 256 mg/l were analysed by DNA sequencing. In ParC one kind of amino acid substitution (of Ser-85 to Ile) in 9 E. faecalis strains with MICs from 16 to 256 mg/l was observed. In GyrA Ser-84 was changed to one of four different amino acids: Arg, He, Cys or Tyr, however no association between the amino acid type and MIC value was found. The last two substitutions have not been reported to date for E. faecalis. Moreover, our results may suggest that mutations within parC and gyrA are associated with development of a high-level of ciprofloxacin resistance.
13

Comparison of simple methods of methicillin- resistance detection and evaluation of some properties of methicillin-resistant Staphylococcus aureus strains

67%
Trzcinski K., Zareba T., Tyski S., Hryniewicz W.
Acta Microbiologica Polonica
|
1993
|
tom 42
|
nr 1
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