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1

Characteristics of humic acids extracted from soils by the capillary electrophoresis

100%
Grzelakowska A., Christy A.
Polish Journal of Soil Science
|
2003
|
tom 36
|
nr 2
145-152
Humic acids have an important role in the soils. When specific structures of which little is known, are studied, one should use the latest analitycal methods. So far the method of fractionation by electrophoretic mobility has not been very popular in the research studies on humic substances. However, it seems that it is possible to study humification processes and subtle changes in the structure of the material investigated using capillary electrophoresis. On the basis of our experience, some soils representing various types were selected as study material. Electrophoretic separation (by means of buffer solutions with various pH-values) was carried out for the humic acids extracted from the soils selected. The results obtained allowed for the determination of differences in the humic acids structures due to their origin.
2

Capillary electrophoresis for diagnosing purine inherited disorders - two complementary approaches

100%
Adam T., Friedecky D., Fairbanks L. D., Bartak P., Sevcik J.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
|
nr 3
3

GA3 content in young and mature antheridia of Chara tomentosa estimated by capillary electrophoresis

88%
Kazmierczak A., Stepinski D.
Folia Histochemica et Cytobiologica
|
2005
|
tom 43
|
nr 1
4

Using capillary electrophoresis to study methylation effect on RNA-peptide interaction

88%
Mucha P., Szyk A., Rekowski P., Agris P. F.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 3
Methylation of RNA and proteins is one of a broad spectrum of post-trans- criptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNA Phe anticodon stem and loop domain (ASL Phe) containing three of the five natu­rally occurring modifications (2'-O-methylcytidine (Cm32), 2'-O-methylguanine (Gm34) and 5-methylcytidine (m5 C40)) (ASL Phe -Cm32,Gm34,m5 C40) and a 15-amino-acid peptide (named tF 2: Ser1 -Ile-Ser-Pro-Trp5 -Gly-Phe-Ser-Gly-Leu10 -Leu- Arg-Trp-Ser-Tyr15 ) selected from a random phage display library (RPL). A pep- tide-concentration-dependent formation of an RNA-peptide complex was clearly ob­servable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASL Phe shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASL Phe was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49–57)-NH2 (named Tat1: Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg- Arg57-NH2). In the presence of Tat(49–57)-NH2 a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg52->Arg(Me)2, crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.
5
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Content available

Separation of rapeseed globulin by micellar electrokinetic chromatography [MEKC] - a short report

75%
Rybarczyk A., Chavan U. D., Amarowicz R.
Polish Journal of Food and Nutrition Sciences
|
2007
|
tom 57
|
nr 3
353-356
Cruciferin was separated from the rapeseed crude proteins using salting out with ammonium sulphate and Sephadex G-200 gel filtration. Then, so obtained protein fraction was separated using a micellar electrokinetic chromatography (MEKC) with SDS as a the surfactant. Nine peaks with migration times between 14.33 and 20.48 min were recorded on the chromatogram. The main cruciferin subfractions were characterised with molecular mass of 22 000 and 33 000 determined using MEKC technique. UV spectra showed that cruciferin protein appears as a complex with phenolic acids.
6
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Content available

Development and validation of a capillary electrophoresis method for determination of oxcarbazepine and zonisamide in pharmaceuticals

75%
Paw B., Wos K.
Journal of Pre-Clinical and Clinical Research
|
2008
|
tom 02
|
nr 2
7

Identification of selected siderophores in the Baltic Sea environment by the use of capillary electrophoresis

75%
Kosakowska A., Kupryszewski G., Mucha P., Rekowski P., Lewandowska J., Pazdro K.
Oceanologia
|
1999
|
tom 41
|
nr 4
8

Studies on morphology and metabolism of prothalli during GA3-induced formation of antheridia in Anemia phyllitidis

75%
Kazmierczak A.
Acta Physiologiae Plantarum
|
1998
|
tom 20
|
nr 3
In Schizaeaceae ferns, including Anemia phyllitidis, formation of antheridia is known to be induced by exogenously applied gibberellic acid. Also present studies show that GA₃ (10⁻⁵ mol·dm⁻³) modifies the development of gametophytes of Anemia phyllitidis. Simultaneously with formation of antheridia, they exhibit lower number of cells but only slightly lowered profile areas and lengths of prothalli. Growth in size of individual cells compensates for lowered division frequency. Cytophotometric measurements reveal no essential changes in the DNA content in vegetative cells of the control and GA₃-stimulated gametophytes. It remains at haploid level and therefore it is assumed that cell cycle is blocked at G1 phase. Application of GA₃ increases the total amount of proteins. CZE (Capillary Zone Electrophoresis) separation of peptides extracted from control and GA₃-treated prothalli indicates the differences in the ratio of their particular forms. In GA₃-treated gametophytes the activities of acid and basic phosphatases, contents of carbohydrates (glucose, starch), chlorophyll, the number of chloroplasts and dry mass of prothalli are increased. GA₃-intensified metabolism, evidenced in gametophytes of A. phyllitidis, may be interpreted as a stimulatory mechanism which influences metabolic pathways involved in forming, developing and maturing of male sex organs.
9

The effect of DNA labeling with the fluorescent dyes R110 and R6G on genotype analysis using capillary electrophoresis

75%
Khan H. A.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr 2
10
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Content available

Optimisation of conditions for capillary zone electrophoresis [CZE] separation of trichloroacetate extracts of salted fish meat

63%
Felisiak K.
Polish Journal of Food and Nutrition Sciences
|
2007
|
tom 57
|
nr 2
177-184
Effects of CE parameters and modifying substances on the quality of separation of trichloroacetic (TCA) extracts of salted Baltic herring meat were studied. The optimal separation parameters were as follows: capillary length 36 cm; voltage 20 kV; injection pressure 2 psi*s; capillary temperature 35°C, detection wavelength 200 nm. Buffer enrichment with 20% acetonitrile and 40% methanol resulted in a slight improvement of the separation quality, while 20 mmol/L triethylamine (TEA) produced some improvement, but was a source of an additional peak in field A. Salt concentration
11

Capillary electrophoresis in analysis of agrochemicals

63%
Kluczyk A.
Pestycydy
|
2004
|
nr 3-4
12

Investigation of a wide spectrum of inherited metabolic disorders by 13C NMR spectroscopy

63%
Bal D., Kraska-Dziadecka A., Gradowska W., Gryff-Keller A.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 1
High-resolution  1H NMR spectroscopy of body fluids has proved to be very useful in diagnostics of inherited metabolic diseases, whereas  13C NMR remains almost unexploited. In this paper the application of  13C NMR spectroscopy of fivefold concentrated urine samples for diagnosis of selected metabolic diseases is reported. Various marker metabolites were identified in test urine samples from 33 patients suffering from 10 different diseases, providing information which could be crucial for their diagnoses. Spectra were accumulated for 2 h or overnight when using spectrometers operating at 9.4 or 4.7 T magnetic fields, respectively. Interpretation of the measurement results was based on a comparison of the peak positions in the measured spectrum with reference data. The paper contains a table with  13C NMR chemical shifts of 73 standard compounds. The method can be applied individually or as an auxiliary technique to  1H NMR or any other analytical method.
13

Simultaneous determination of six quinolone antibiotics in poultry and porcine samples by capillary electrophoresis

63%
Kowalski P., Plenis A.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 1
A capillary electrophoresis (CE) method for the simultaneous determination of residues of six quinolones (enrofloxacin, ciprofloxacin, ofloxacin, lomefloxacin, norfloxacin, cinoxacin) in chicken, hen, and swine tissue samples were developed and validated. The sample preparation consisted of a solid phase extraction on C-18 cartridges prior to the analysis by CE with UV detection. The method was validated in terms of selectivity, linearity, precision, accuracy, recovery, and stability. The calibration curves were linear to at least 10-1 000 ng/g for all quinolones with r² > 0.999. The values of decision limits (CCα) and detection capabilities (CCβ) for the analysed substances were between 3.2-16.9 and 3.5-20.3 ng/g, respectively. The CE method was robust and specific, allowing reliable quantification of quinolone residues in animal tissues and should also be useful for clinical and biomedical investigations.
14

GA3 content in antheridia of Chara vulgaris at the proliferative stage and in spermiogenesis estimated by capillary electrophoresis

63%
Kazmierczak A., Kwiatkowska M., Poplonska K.
Folia Histochemica et Cytobiologica
|
1999
|
tom 37
|
nr 1
15

Capillary electrophoresis as a tool for determining the level of orotic acid and uracil in patients with urea cycle defects

63%
Salerno C., D'Eufemia P., Celli M., Finocchiaro R., Crifo C., Giardini O.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
|
nr 3
16

Pozostalosci kokcydiostatykow w tkankach jadalnych drobiu

51%
Kowalski P., Oledzka I.
Bromatologia i Chemia Toksykologiczna
|
2005
|
tom 38
|
nr Supl.
391-394
Opracowano metodą elektroforetycznego oznaczania pozostałości nifursolu w tkankach jadalnych indyków (mięśnie, skóra z tłuszczem, wątroba i nerki). Przeprowadzono walidacją całej procedury oznaczania nifursolu; limit wykrywalności ustalono na 7 g/kg w badanych tkankach. Przedstawiona metoda jest specyficzna, precyzyjna, czuła i wystarczająco dokładna do oznaczania pozostałości leków weterynaryjnych w tkankach pochodzenia zwierzęcego.
17

Opracowanie metody oznaczania iwermektyny w jadalnych tkankach zwierzat rzeznych

51%
Oledzka I., Kowalski P.
Bromatologia i Chemia Toksykologiczna
|
2005
|
tom 38
|
nr Supl.
387-390
Opracowano metodą oznaczania iwermektyny w surowicy i tkankach jadalnych zwierząt z zastosowaniem elektroforezy kapilarnej, którą poddano ocenie statystycznej. Proces walidacji uwzględniał następujące elementy: liniowość w zakresie od 1 do 20 µg/kg, czułość, specyficzność, dokładność i precyzję metody. Odzysk analitu w procesie ekstrakcji wynosił 80,2%. Granicę wykrywalności ustalono na 0,3 µg/kg a oznaczalności ilościowej na 1 µg/kg.
18

Analysis of ferritin-type proteins in the hepatopancreas of Baltic blue mussel [Mytilus trossulus]

51%
Kozuch J., Pempkowiak J., Kupryszewski G., Mucha P., Rekowski P., Smol J.
Oceanologia
|
1997
|
tom 39
|
nr 2
19

Application of capillary electrophoresis for DNA-protein binding tests

51%
Kazmierczak A., Kazmierczak J. M.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 1
20

Preparation of soil and sediment samples for determination of organometallic compounds

51%
Dubiella-Jackowska A., Wasik A., Przyjazny A., Namiesnik J.
Polish Journal of Environmental Studies
|
2007
|
tom 16
|
nr 2
Organometallic compounds are widely used in almost all sectors of industry. As a result of emissions from natural and anthropogenic sources they are released to the environment, where they undergo a variety of conversions and interactions. Some organometallic compounds exhibit high toxicity. Organometallic compounds of mercury, tin, lead, selenium and arsenic are among the most common and hazardous compounds. Based on literature dealing with the occurrence and methods of determination of selected organometallic compounds of mercury, tin, lead, selenium and arsenic, we describe their sources, uses and the mechanism of toxicity. Fundamental problems associated with speciation analysis are discussed and the basic steps of analytical procedure used for the determination of organometallic compounds of Hg, Sn, Pb, Se, and As in soil and bottom sediments, including sample collection, preservation, storage, extraction, separation and determination, are characterized.
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