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The paper presents a study on the effect of soil matric suction on the variation of leaf chlorophyll index and nitrogen concentration of canola. Results showed that chlorophyll index increases exponentially with soil matric suction, especially at the late season of canola growing time. At moderate matric suction (200 and 300 kPa soil suction heads), chlorophyll index remains nearly constant, but in drier soil (matric suction >300 kPa),chlorophyll index increases gradually with time. Despite the va-riation of the total leaf nitrogen with the soil matric suction, it is similar to the variation of the chlorophyll index, but the results showed that the chlorophyll index – nitrogen concentration curve has a de-marcated bi-modal shape. We suggest that 2.7% of nitrogen and 69.8 of the chlorophyll index value represent the upper limit of the chlorophyll meter reliability for estimation of canola nitrogen under a wide range of soil moisture levels. These results confirm that the chlorophyll meter can be used as an effective tool for rapid and non-destructive estimation of the relative chlorophyll and nitrogen content in canola leaves at a wide range of soil moisture content, except for nearly wilting coefficient or extremely high drought stress.
A life table can be used as an important and appropriate tool to evaluate the susceptibility or resistance level of different host plant cultivars to insect pests. In the current study, we determined the suitability or inferiority of five different canola cultivars (Hayula420, Hayula401, Hayula50, Hayula60, RGS) to Nysius cymoides, under laboratory conditions. Data were analysed based on the age-stage, two-sex life table theory. Nysius cymoides which fed on Hayula420 had the longest nymphal period, while those which fed on Hayula50 had the shortest nymphal period. Developmental times (sum of incubation and nymphal periods) was longest for those which fe d on Hayula420 and the shortest for those which fed on Hayula50. The adult pre-oviposition period (APOP), total pre-oviposition period (TPOP), mean fecundity, and adult longevity of adults reared on different canola cultivars showed significant differences. The highest and lowest net reproductive rates (R0) were obtained for those which fed on Hayula420 (11.40 offspring per individual) and Hayula401 (5.47 offspring per individual), respectively. The highest value (0.0395 d–1) for the intrinsic rate of increase (r) was obtained for those which fed on Hayula 60 cultivar and the lowest value (0.0261 d–1) for those which fed on Hayula401 cultivar. The shortest and longest mean generation times (T) were obtained for those which fed on RGS and H401 cultivars, respectively. The lowest and highest values of life expectancy (exj) were obtained for those which fed on RGS and Hayula420 cultivars, respectively. The results showed that Hayula401 and RGS were not susceptible cultivars to N. cymoides. These cultivars showed higher resistance to N. cymoides, while Hayula60, Hayula420, and Hayula50 were found to be suitable cultivars but with lower resistance to N. cymoides, respectively.
Parasitic weeds especially Phelipanche aegyptiaca decrease severely the production of canola. This study evaluated the effect of intercropping different wheat genotypes with canola on Phelipanche aegyptiaca growth. Ten wild wheat genotypes with different ploidy levels including TRI11712, TRI19322, TRI18664, TRI19652, TRI565, TRI15593, TRI12911, TRI11554, TRI17606, TRI7259P and seven cultivated bread wheats, namely: Falat, Chamran, Alamut, Baiat, Kavir, Sepahan, Alvand in addition to a canola cultivar called Zarfam were studied. The results revealed that intercropping of canola with wheat could significantly reduce broomrape growth depending on the type of wheat genotype. A significant genetic variation of allelopathic activity in wheat was observed, indicating the contribution of multiple genes conferring the allelopathic trait. TRI565 and TRI12911, TRI15593, TRI18664, TRI19652, TRI17606, TRI19322, and TRI7259 genotypes showed strong inhibitory effects and can be considered as potential allelopathic genotypes to suppress broomrape. The inhibitory potential of wild wheat genotypes was stronger than cultivated wheat genotypes. Alamut, Baiat, Alvand, Sepahan, and TRI11712 possessed strong stimulatory effects on broomrape germination. Such genotypes may be valuable as trap crops for depleting the Egyptian broomrape seed bank.
The effect of laser priming at different irradiation times on canola yield and its components under saline conditions were investigated. The results showed that laser priming had a positive effect on yield and its components and caused yield increase under saline conditions. Increase in salt levels had a negative and significant effect on seed yield, number of seeds per pod, number of pod per plant, pod length and plant height. The results showed that 45-min laser priming had the strongest effect on yield and yield components and reduced significantly the adverse effects of salinity.By contrast, laser radiation applied for 60 and 75 min, resulted in a dramatic decrease in yield and its components. Correlation coefficients between the attributes showed that canola yield had a positive and significant correlation with plant height, number of seeds, pod per main branch and lateral branches, length of pod and number of lateral branches. Effects of laser and salinity were significant on lateral branch pod length but not on main branch pods.
Condensed tannins and phenolic acids were extracted from canola hulls into acetone-water (70:30; v/v) and fractionated using Sephadex G-25 and G-50 column chromatography with 50% (v/v) acetone as the mobile phase. Three fractions containing phenolic compounds were obtained from the extract using Sephadex G-25 and four using Sephadex G-50. The latter fractions eluted from the columns, packed with either gel, both gels were free of condensed tannins. The contents of total phenolics and condensed tannins were determined in the range of 52–280 mg/g, 0.198–1.153 A500/mg (vanillin method), and 0.333–1.330 A550 /mg (after n-butanol/HCl hydrolysis). The method described can be used as a step in the purification of condensed tannins of canola hulls before semi-preparative RP-18 HPLC column chromatography.
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