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There was developed a little-work-consuming and non-expensive microdiffu- sion method of carboxyhaemoglobin determination in bloody in low range concentration. It can be used in the assessment of passive smokers' exposure to carbon monoxide. The amount of blood neccessary for the determination was 0.15 ml. An average precision of the developed method of the carbon monoxide determination (converting into HbCO) for the concentration ranged from 1 to 5%, from 3 to 12% and from 5 to 20% was respectively 5.96, 4.09 and 2.14.
The goal of this study was the assessment of the influence on both insulin and growth hormone levels in lambs blood of blood meal and Wroclaw product - dry brown livex (modified by whey). Experiments were carried out on 30 young Polish "wielkopolska" lowland - sheep - wool type, aged 3-4 months. Livex and blood meal constituted 2% of forage. Blood samples for radioimmunologic examinations (GH and insulin level determination) were taken 1 hour before feeding, directly before feeding and 1.0, 1.5, 2.0, 3.0, 5.0, 6.0 and 8.0 hours afterwards. During the experiment higher levels of insulin and GH were observed in animals fed with livex and forage mixture than in animals fed with blood meal and forage mixture. Livex caused a bigger body mass increase (mean increase 27.3%) in comparison with animals fed with control forage. Although neither livex nor blood meal are hormone preparations, changes in insulin and growth hormone levels were observed.
Suicides committed under the alcohol constitute on average 20% of all suicidal deaths. Alcohol is a signifant factor of suicidal risk. The assessment of the role of alcohol in the decision to commit suicide is in many cases difficult. Only in the case of the suicidal death of an alcoholic the circumstances of the influence of alcohol on self-destructive behavior is not as important, unless it is a means to "get rid" of alcoholism. As rule alcohol is drunk to gain courage — to make the decision to commit suicide easier.
The objective of the work was to explain to what degree fluorine bioaccumulation is conditioned by the high level of industrialisation in Central Pomerania depending on the distance of a farm from the place of contamination emission in the trophic system: soil - plant - animal. Soil, feed, and horsehair and blood samples were taken from each level of the system. Material sampled from 48 crossbred horses was divided into three age groups: group 1 - horses aged 7 years and below, group 2 - horses aged between 7 and 15 years, and group 3 - horses aged over 15 years. Fluorine measurements in the soil, feed, and examined tissues were taken by means of an ion-selective electrode combined with an ORION ion meter. An elevated soil acidity in zone I, in the mouth of the Vistula River, stretching 50 km west of the place of contamination emission, increased the level of this element in the soils of the examined area, as well as in feeds offered to horses, compared with zone, II located in the mouth of the Leba and the Slupia Rivers, 80 to 100 km to the west of the place of contamination emission. A significantly higher average fluorine concentration in the blood and hair of horses raised in zone I, compared with zone II, was found. This concentration was the highest in the youngest animals. Fluorine accumulation in the blood and in the hair of horses raised in zone II was found to increase as the animals grew. Monitoring of fluorine bioaccumulated in soil, feed, blood, and the hair of horses may be a useful indicator of fluorine contamination in the natural environment.
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