The intensive use of acetolactate synthase (ALS) inhibitors for crop protection caused in many cases weed resistance to these herbicides. Resistant to sulfonylureas biotypes of cornflower appeared in Poland. During 2006–2009 seeds of cornflower were collected from 15 field were farmers informed that after using herbicides the control of this weed was very poor. These collected seeds were used at greenhouse experiments. Collected biotypes were compared with susceptible biotype from Experimental Station Strzeszyn near Poznań. Obtained data from the experiments indicated that two tested biotypes of C. cyanus were cross-resistant with two ALS inhibiting herbicides tribenuron methyl and chlorsulfuron. The calculation for dose-response curve and resistance index ED50 indicated that the level of resistance of these biotypes was high.
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