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1

Cytochemical study of dehydrogenase activity in two euglenid species of the genus Parastasia Michajlow,1966

100%
Wita I.
Acta Protozoologica
|
1989
|
tom 28
|
nr 1
2

Beta-hydroxybutyrate, glucose, calcium, phosphorus, and vitamin C concentrations in blood of dairy cows with subclinical ketosis during the early lactation

100%
Zhang Z., Liu G., Li X., Wang Z., Kong T., Zhang N., Guo C.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 1
71-74
The objective of this study was to examine the association between β-hydroxybutyrate (BHBA) and glucose, calcium (Ca), phosphorus (P), and vitamin C concentrations in dairy healthy and with subclinical ketosis cows during an early lactation period. The blood from 99 healthy cows and 26 sub-clinically ketotic cows within the first two months of lactation was sampled. Serum concentration of BHBA was measured by enzymatic-rate method, glucose by oxidase method, and P by colorimetric method. These tests were performed in a biochemical auto-analyser. Serum Ca concentration was measured by atomic absorption spectrometer followed by acid digestion. Vitamin C was measured by HPLC. The results showed that in ketotic cows serum BHBA was significantly increased (P<0.01) and glucose and Ca concentrations were significantly decreased (P<0.01) as compared to healthy cows, while vitamin C and P concentrations were similar in the both groups of cows (P>0.05). The serum BHBA concentration was negatively correlated with the serum glucose and Ca concentrations (P<0.01). Vitamin C and P status of cows with subclinical ketosis was not affected by BHBA. Serum Ca concentration can be used as a reference index for the diagnosis of subclinical ketosis.
3

Evaluation on serum glucose, BHB, urea and cortisol concentrations in pregnant ewes

100%
Ramin A. G., Siamak A.-R., Macali S.-A.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 06
674-677
A study was conducted to determine the distribution of subclinical pregnancy toxemia by the evaluation of serum glucose, BHB, urea, and cortisol concentrations in pregnant ewes. Eight hundred and nine blood samples were collected from 497 pregnant, 242 lambed, and 70 aborted ewes. The concentrations of BHB, glucose and urea were assessed by spectrophotometer and cortisol by ELISA. Mean urea and BHB concentrations in pregnant ewes were greater and glucose was lower than in lambed and aborted ewes. Cortisol concentrations in pregnant ewes were higher than lambed but lower than aborted ewes. Except for BHB, the mean comparison of parameters showed a significant difference (P < 0.01) in that the concentrations were similar in all groups. A negative correlation (P < 0.05) in urea concentration was observed between pregnant and lambed ewes. There were correlations (P < 0.01) between BHB and glucose in pregnant ewes, BHB and cortisol in lambed ewes and BHB and urea in aborted ewes. Regarding the references, the concentration of BHB > 0.7 mmol/l is considered as subclinical pregnancy toxemia; the distribution and percent of ewes in pregnant, lambed and aborted groups were 61 (12.3%), 17 (7%) and 6 (8.57%), respectively. Similarly, for glucose < 20 mg/dl were 76 (15.3%), 38 (15.7%), 10 (14.3%) and for cortisol > 52 ng/ml were 28 (8.54%), 1 (0.6), 5 (10.2%), respectively. Significant differences were found for the BHB and cortisol groups. The highest subclinical pregnancy toxemia occurred among pregnant and aborted ewes. Mean comparison of parameters in the group with BHB > 0.7 mmol/l showed a significant difference (P < 0.01) for urea and cortisol concentrations and in the group of glucose < 20 mg/dl and cortisol > 52 ng/ml showed differences only for the BHB concentration. Thus it is concluded that hypoglycemia, uremia and high cortisol concentration in late pregnancy could be considered as subclinical pregnancy toxemia up to 12% that should be seriously considered in order to support prevention or enact treatment.
4

The level of serum ionised calcium, aspartate aminotransferase, insulin, glucose, betahydroxybutyrate concentrations and blood gas parameters in cows with left displacement of abomasum

100%
Sen I., Ok M., Coskun A.
Polish Journal of Veterinary Sciences
|
2006
|
tom 09
|
nr 4
The purpose of this study was to determine concentrations of serum glucose, aspartate aminotransferase (AST), insulin, beta-hydroxybutyrate (BOH) and ionised calcium (lea) in dairy cows with left displaced abomasum (LDA) and to compare these parameters before and after surgical correction of LDA. Eighteen Swiss-Holstein dairy cows with LDA were used in this study. Clinically healthy post parturient cows (n: 10) from a local dairy farm were used as control group. Blood samples were collected from the jugular vein from all the cows. Surgery was performed in cows with LDA. Blood samples from cows with LDA were collected 24 hours after surgery. The abomasum was repositioned followed by an omentopexy. Six of the 18 cows with LDA had clinical ketosis as detected with urine dipstick. The mean concentrations of insulin, BOH, glucose and AST in cows with LDA at admission time were increased compared with the healthy cows. But the mean concentration of ICa at admission time was slightly decreased compared with healthy cows. The mean BOH concentration was decreased 24 hours following surgery compared with values on admission time. However, the mean serum AST levels were increased both at admission time and 24 hours after surgery compared with healthy cows. The levels of blood gas parameters in cows with LDA were not significantly different in comparison with healthy cows, although hyperbasemia in six of 18 cows with LDA was determined. In conclusion, the results of this study indicated that serum insulin, glucose, AST and BOH levels were increased in dairy cows with LDA. Serum BOH and ICa levels were decreased 24 hours after surgery compared with values on admission time. All cows with LDA used in this study had subclini- cal/clinical ketosis. We could say that ketosis might be a risk factor for the displacement of the abomasum.
5

Occurrence, purification and properties of the staphylococcal beta-hydroxybutyrate dehydrogenase

100%
Szewczyk E., Rozalska M.
Acta Microbiologica Polonica
|
1994
|
tom 43
|
nr 1
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