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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Biological degradation of metribuzin and profenofos by some efficient bacterial isolates

100%
Tamilselvan C., Joseph S. J., Mugunthan G., Sathish Kumar A., Syed Musthaq Ahamed S.
International Letters of Natural Sciences
|
2014
|
tom 09
The soil sample was collected from the paddy field of Sriperumbudur, Tamilnadu which is having a history of repeated pesticide applications. The isolation of efficient pesticide degrading bacteria was identified as Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The growth of the three pesticide degrading isolates was assessed in Minimal salt broth containing 25 ppm of pesticides. Two popularly used pesticides Metribuzin and Profenofos were selected for this study. Among the three bacterial isolates, the bacteria Bacillus subtilis utilized the pesticides effectively and showed maximum growth. The growth of the three pesticides degrading isolates Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was assessed in Minimal salt broth containing 25 ppm of pesticides at different temperature levels (25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C & 55 °C) and pH levels (pH 4, pH 5, pH 6, pH 7 & pH 8) and carbon sources (Lactose, Dextrose, Fructose, Mannose & Galactose) and nitrogen sources Peptone, Yeast extract, Beef extract, Malt extract and Casein respectively. The maximum growth rate of bacteria was recorded at 35 °C and pH 6. The maximum growth of bacteria was in the presence of Dextrose followed by Fructose, Galactose and Mannose. The least growth was recorded in Lactose broth culture. The maximum growth of bacteria was in the presence of Malt extract followed by Peptone, Yeast extract and Casein. The least growth was recorded in Beef extract broth culture. The bacterial isolates showed maximum growth in the Minimal salt broth containing Profenofos followed by Metribuzin.
2
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Pulsed-field gel electrophoresis as a molecular tool for characterizing genomes of certain food-borne bacterial isolates - a review

100%
Pal P.
International Letters of Natural Sciences
|
2015
|
tom 02
The evolutionary transition from phenotypic to molecular analysis of infectious disease in bacterial epidemiology led to the search for suitable approaches to ascertain genomic relatedness or heterogeneity between bacterial clinical isolates. Pulsed-field gel electrophoresis (PFGE) technique was developed for separating and analyzing long DNA fragments of several megabases in alternating electric field. Comparison of electrophoresis profiles of restriction enzyme-digested genomic DNA from bacterial isolates has proved to be a useful epidemiological tool for genetic discrimination of bacterial strains, detection of genetic relatedness, to locate the source of outbreak and to monitor the spread of the microorganisms in endemic zones. PFGE is considered as a gold standard method for typing of bacterial isolates because of the remarkable endurance of this technique as a typing method for the last 20 years in molecular epidemiology. In this current review the pros and cons of PFGE use in current molecular microbiological research are explored in the context of determination of genome organization of certain food-borne bacterial isolates causing infectious diseases in human beings.
3

Variability in Xanthomonas oryzae pv. oryzae, the incitant of bacterial blight disease of rice

84%
Nayak D., Reddy P. R., Nayak P.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 1
15-26
The virulence pattern of 52 bacterial strains of Xanthomonas oryzae pv. oryzae, the causal organism of bacterial blight disease of rice was assessed on 41 rice genotypes including five Japanese and five Philippines' differentials. A significant differential interaction observed among the bacterial isolates, the host-genotypes and in their interaction suggested that the host-genotypes differed in vertical resistance and bacterial isolates differed in virulence. The two Japanese differentials Kinmaze and Rantai Emas and two Philippines' differentials IR 8 and IR 20, exhibited highly susceptible reactions against all the 52 bacterial isolates. Five new Indian differentials were selected, one from each of the five clusters of genotypes obtained through hierarchical method of numerical analysis of the virulence pattern of 52 bacterial isolates on 41 host-genotypes. The 52 bacterial isolates could be grouped into six clusters on the basis of their pathogenicity pattern on five new Indian differentials, which were designated as Pathotype-1, 4, 7, 14, 15 and 16, following a standard computer generated virulence pattern chart. These pathotypes were comparable with the Japanese pathotype groups of I, II, III and IV and Philippines' pathotype groups of I, II, III, IV and V. The most virulent pathotype-1 was distributed over four eastern states of India, namely Andhra Pradesh, Orissa, West Bengal and Bihar. In view of the free exchange of genetic material all over the country, continuous monitoring of the prevalence of new pathotypes with the help of the present set of differentials, will accelerate the resistance breeding programme and help in disease control through introduction of location specific resistant cultivars.
4

The effect of cyanogenic strains of Pseudomonas fluorescens on plant growth

67%
Piotrowska-Seget Z.
Acta Microbiologica Polonica
|
1995
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tom 44
|
nr 2
5

Biochemiczna charakterystyka izolatow bakterii raka pomidora [Clavibacter michiganensis subs.michiganensis]

59%
Kamasa J., Pospieszny H.
Progress in Plant Protection
|
2002
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tom 42
|
nr 2
773-776
6

Selected enzymatic properties of bacterioplankton in lakes of various degrees of eutrophication

59%
Wcislo R., Chrost R. J.
Acta Microbiologica Polonica
|
1997
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tom 46
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nr 2
7

Azotobacter chroococcum and Streptomyces atroolivaceus as biocontrol agents of Xanthomonas campestris pv. malvacearum

51%
El-Shanshoury A. R.
Acta Microbiologica Polonica
|
1994
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tom 43
|
nr 1
8

Skuteczność mieszaniny bakterii Bacillus i Pseudomonas spp. w ochronie cyprysika Lawsona przed Phytophthora cinnamomi

43%
Ksiezniak A., Wroblewska B.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 529
Przeprowadzono badania nad możliwością ograniczania fytoftorozy cyprysika Lawsona przy zastosowaniu bakteryjnych izolatów uzyskanych z ryzosfery i wykazujących w testach laboratoryjnych antagonizm wobec Phytophthora cinnamomi. Wykazano, że mieszanina drobnoustrojów stosowana przed sadzeniem cyprysika Lawsona do inokulowanego podłoża, istotnie ograniczała rozwój fytoftorozy w ciągu 10-tygodniowej uprawy. Zastosowanie bakterii do opryskania roślin spowodowało słabsze ograniczenie rozwoju fytoftorozy po 10 tygodniach uprawy.
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