Wyniki wyszukiwania

1

Fluctuating membrane pores - a possible mechanism for the action of the antibiotic peptide KLA1

100%

Kim D., Dathe M., Bezrukov S., Hauss T., Dante S., Klosgen B.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.

2

The role of cathelicidin LL-37 in cancer development

86%

Piktel E., Niemirowicz K., Wnorowska U., Watek M., Wollny T., Gluszek K., Gozdz S., Levental I., Bucki R.

Archivum Immunologiae et Therapiae Experimentalis

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2016

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tom 64

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nr 1

3

Developmental gene expression of antimicrobial peptide Protegrin-1 and effect of weaning on gene regulation of Protegrin-1 in piglets

86%

Han F. F., Wang Y. Z., Feng J., Guo J., Xu Z. R.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 1

86-95

4

Review of the expression of antimicrobial peptide defensin in honey bees Apis mellifera L.

86%

Ilyasov R. A., Gaifullina L. R., Saltykova E. S., Poskryakov A. V., Nikolenko A. G.

Journal of Apicultural Science

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2012

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tom 56

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nr 1

5

Effect of Pseudomonas aeruginosa crude proteolytic fraction on antibacterial activity of Galleria mellonella haemolymph

86%

Andrejko M.

Folia Biologica

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2004

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tom 52

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nr 1-2

6

The effect of temporin A on limitation of growth and development of Phoma narcissi

86%

Kamysz W., Saniewska A., Greber K., Lukasiak J.

Pestycydy

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2004

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nr 3-4

7

Bacterial cell surface structures in Yersinia enterocolitica

72%

Bialas N., Kasperkiewicz K., Radziejewska-Lebrecht J., Skurnik M.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

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nr 3

8

Cathelicidin LL-37: a multitask antimicrobial peptide

72%

Bucki R., Leszczynska K., Namiot A., Sokolowski W.

Archivum Immunologiae et Therapiae Experimentalis

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2010

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tom 58

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nr 1

9

Varroa destructor induces changes in the expression of immunity-related genes during the development of Apis mellifera worker and drone broods

72%

Zaobidna E. A., Zoltowska K., Lopienska-Biernat E.

Acta Parasitologica

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2017

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tom 62

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nr 4

10

Magainin 2 interaction with Pantoea agglomerans lipopolysaccharide

72%

De Lucca A. J., Jacks T. J., Brogden K. A.

Annals of Agricultural and Environmental Medicine

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1996

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tom 03

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nr 1

11

Expression of natural antimicrobial peptide beta-defensin-2 and Langerhans cell accumulation in epidermis from human non-healing leg ulcers

72%

Galkowska H., Olszewski W. L., Wojewodzka U.

Folia Histochemica et Cytobiologica

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2005

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tom 43

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nr 3

12

LL-37 but not 25-hydroxy-vitamin D serum level correlates with healing of venous leg ulcers

58%

Krejner A., Litwiniuk M., Grzela T.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

|

nr 5

13

Toll-like receptors in human papillomavirus infection

58%

Zhou Q., Zhu K., Cheng H.

Archivum Immunologiae et Therapiae Experimentalis

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2013

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tom 61

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nr 3

14

Purification and characterization of antibacterial proteins from granular hemocytes of Indian mud crab, Scylla serrata

58%

Yedery R. D., Reddy K. V. R.

Acta Biochimica Polonica

|

2009

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tom 56

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nr 1

71-82

Marine invertebrates depend upon antimicrobial peptides (AMPs) as a major component of innate immunity, as they are rapidly synthesized and diffuse upon pathogen invasion. In this study, we report the identification and characterization of a 11 kDa antimicrobial protein, which we name SSAP (for Scylla serrata antimicrobial protein), from granular hemocytes of the mangrove crab S. serrata. The protein is highly similar to scygonadin, a male-specific AMP isolated from the ejaculatory duct of S. serrata. SSAP was isolated using various chromatographic techniques, viz. ion-exchange, ultra filtration and RP-HPLC, and demonstrated antibacterial activity against Gram positive and Gram negative bacteria. Full length mRNA encoding SSAP was amplified using a combination of RT-PCR and RACE. The nucleotide sequence revealed a full-length ORF of 381 bp coding for a preprotein of 126 amino acids comprising a signal peptide of 24 amino acids and a mature protein of 102 amino acids with a predicted mass of 11435 Da and pI of 5.70. Unlike scygonadin, SSAP is expressed in several tissues of both male and female crabs, as evidenced by RT-PCR, Northern and Western blot analyses. The study suggests that SSAP might be an isoform or a variant of scygonadin and might play an important role in regulating the immunity of the crab upon microbial infection.

15

Antitumor activity of antimicrobial peptides against U937 histiocytic cell line

58%

Koszalka P., Kamysz E., Wejda M., Kamysz W., Bigda J.

Acta Biochimica Polonica

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2011

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tom 58

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nr 1

We investigated cytotoxic activity of antimicrobial peptides of different origin (both naturally occurring and synthetic), structure and known mechanisms of action against human histiocytic lymphoma cell line U937. The strongest cytotoxic activity against U937 cell line was shown by Pexiganan MSI-78, followed by Citropin 1.1, Protegrin 1 and a synthetic lipopeptide, N-α-palmitoyl-l-lysyl-l-lysine amide (Pal-Lys-Lys-NH2). The cytotoxic activity of the peptides was more dependent on the time of incubation than concentration. Only for the lipopeptide, whose mode of action was restricted to disruption of electric potential of the cell membrane, the correlation between cytotoxicity and concentration was almost linear. The high cytotoxicity of Pexiganan MSI-78, Protegrin 1 and the lipopeptide could be basically explained by their membranolytic activity leading to necrosis. However, in the case of Citropin 1.1, the cell membrane integrity was disrupted only slightly and independently of the peptide concentration. Therefore, some other mechanism of action might be responsible for its strong dose-dependent cytotoxic activity, e.g., membranolytic activity leading to apoptosis. Furthermore, TNF-α production due to LPS (lipopolysaccharide) stimulation was suppressed by the presence of Citropin 1.1, Pexiganan MSI-78 or Protegrin 1, but not by Buforin 2 or the lipopeptide. Our experiments have shown that cytotoxic activity is not limited to some specific molecular structure of a peptide, but rather to the length of the peptide chain as it is likely to affect the efficiency of the tumor cell membrane disruption and interaction with LPS.

16

Characteristic of bacteriocines and their application

58%

Karpinski T. M., Szkaradkiewicz A. K.

Polish Journal of Microbiology

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2013

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tom 62

|

nr 3

Bacteriocines are small peptides with anti-bacterial properties. They are produced both by Gram-positive and Gram-negative bacteria. Until now, a few hundred bacteriocines were described. Classification of bacteriocines undergoes continuous alterations, as new developments regarding their structure, amino acid sequence and recognised mechanism of their action are available. Some of bacteriocins (lantibiotics) contain atypical amino acids, such as lantionine (Lan), methyllantionine (MeLan), dehydroalanine (Dha), dehydrobutyrine (Dhb), or D-alanine (D-Ala). The best recognized bacteriocines are produced by lactic acid bacteria, including nisine produced by strains of Lactococcus lactis. These bacteriocines have been recognized to be fully safe for humans. At present, nisine is used in food industry, as a preserving agent. Other lactic acid bacteria bacteriocines and probiotic preparations provide an alternative for antibiotics, and are used in food and in animal feed.

17

In vitro activity of synthetic antimicrobial peptides against Candida

58%

Kamysz W., Nadolski P., Kedzia A., Cirioni O., Barchiesi F., Giacometti A., Scalise G., Lukasiak J., Okroj M.

Polish Journal of Microbiology

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2006

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tom 55

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nr 4

Yeast-like fungi are the most common cause of fungal infections in humans. Actually, in the age of opportunistic infections and increasing resistance, development of modern antifungal agents becomes a very important challenge. This paper describes synthesis and antimicrobial assay of four naturally occurring peptide antibiotics (aurein 1.2, citropin 1.1, temporin A, uperin 3.6) and three chemically engineered analogues actually passing clinical trials (iseganan, pexiganan, omiganan) against Candida strains isolated from patients with infections of the oral cavity or respiratory tract. The peptides were synthesized using solid-phase method and purified by high-performance liquid chromatography. Biological tests were performed using the broth microdilution method. The antifungal activity of the peptide antibiotics was compared to that of nystatin and amphotericin B. We found synthetic peptides to be generally less potent than amphotericin B or nystatin. However, some of the naturally occurring peptides still retained reasonable antifungal activities which were higher than these of iseganan, pexiganan or omiganan. We think that the naturally occurring peptide antibiotics included in our study can be a good matrix for development of novel antifungal compounds.

18

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Cathelicidin LL-37: LPS-neutralizing, pleiotropic peptide

58%

Golec M.

Annals of Agricultural and Environmental Medicine

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2007

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tom 14

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nr 1

Human organism, constantly exposed to a large variety of pathogenic microorganisms and their products, such as lipopolysaccharide (LPS), developed innate immunity as a fi rst line of defence. One of the compartments of our organism well equipped with these defence mechanisms is the respiratory system. The cells lining the airways respond to the presence of virulent microorganisms by producing natural antimicrobial peptides, including the only member of the cathelicidins family found to date in humans, peptide LL-37. LL-37 is a small peptide of 37 amino acid residues. The peptide, in addition to its bactericidal effect, plays numerous roles in infl ammatory and tissue remodelling processes. It stimulates angiogenesis, induces proliferation of lung epithelial cells, accelerates wound closure of the airway epithelium, and provokes cytokine release (e.g. IL-8) and cell migration. LL-37 is also able to neutralize LPS, a heteropolymer associated with organic dust, produced by Gram-negative bacteria. LPS (commonly referred to as endotoxin) plays an important role in pathogenesis of many respiratory diseases caused by organic dust, including organic dust toxic syndrome and chronic illnesses such as chronic obstructive pulmonary disease (COPD), asthma or allergic alveolitis (hypersensitivity pneumonitis). LPS is a strong pro-infl ammatory stimulus, inducing in respiratory airways expression of antimicrobial peptides, including LL-37, which is in turn a potent LPS-neutralizing factor. The article discusses the complex interplay between endotoxin and the LPS-neutralizing, pleiotropic peptide LL-37 in pathogenic mechanisms of lung diseases, with regard to closer perspectives of using LL-37 and its derivatives as therapeutic agents.

19

Novel properties of antimicrobial peptides

58%

Kamysz W., Okroj M., Lukasiak J.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 2

Endogenous peptide antibiotics are known as evolutionarily old components of innate immunity. Due to interaction with cell membrane these peptides cause permeabilization of the membrane and lysis of invading microbes. However, some studies proved that antimicrobial peptides are universal multifunctional molecules and their functions extend far beyond simple antibiotics. In this review we present an overview of the general mechanism of action of antimicrobial peptides and discuss some of their additional properties, like antitumour activity, mitogenic activity, role in signal transduction pathways and adaptive immune response.

20

Gut microbiota, probiotics and inflammatory bowel disease

51%

Stephani J., Radulovic K., Niess J. H.

Archivum Immunologiae et Therapiae Experimentalis

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2011

|

tom 59

|

nr 3

Ograniczanie wyników

Fluctuating membrane pores - a possible mechanism for the action of the antibiotic peptide KLA1

The role of cathelicidin LL-37 in cancer development

Developmental gene expression of antimicrobial peptide Protegrin-1 and effect of weaning on gene regulation of Protegrin-1 in piglets

Review of the expression of antimicrobial peptide defensin in honey bees Apis mellifera L.

Effect of Pseudomonas aeruginosa crude proteolytic fraction on antibacterial activity of Galleria mellonella haemolymph

The effect of temporin A on limitation of growth and development of Phoma narcissi

Bacterial cell surface structures in Yersinia enterocolitica

Cathelicidin LL-37: a multitask antimicrobial peptide

Varroa destructor induces changes in the expression of immunity-related genes during the development of Apis mellifera worker and drone broods

Magainin 2 interaction with Pantoea agglomerans lipopolysaccharide

Expression of natural antimicrobial peptide beta-defensin-2 and Langerhans cell accumulation in epidermis from human non-healing leg ulcers

LL-37 but not 25-hydroxy-vitamin D serum level correlates with healing of venous leg ulcers

Toll-like receptors in human papillomavirus infection

Purification and characterization of antibacterial proteins from granular hemocytes of Indian mud crab, Scylla serrata

Antitumor activity of antimicrobial peptides against U937 histiocytic cell line

Characteristic of bacteriocines and their application

In vitro activity of synthetic antimicrobial peptides against Candida

Cathelicidin LL-37: LPS-neutralizing, pleiotropic peptide

Novel properties of antimicrobial peptides

Gut microbiota, probiotics and inflammatory bowel disease