Wyniki wyszukiwania

1

Susceptibility testing and resistance phenotypes detection in bacterial pathogens using the VITEK 2 system

100%

Stefaniuk E., Mrowka A., Hryniewicz W.

Polish Journal of Microbiology

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2005

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tom 54

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nr 4

A set of well characterized strains, collected in Polish hospitals, including Gram-negative (n = 93) and Gram-positive (n = 90) isolates was used in the study. The VITEK 2 AST-cards were used in the analysis according to the manufacturer's recommendations. Comparison of the susceptibility data obtained by the standard method and by VITEK 2 cards proved concordant in 99% of cases. Clinically important mechanisms were revealed by the VITEK 2 AES with > 95% agreement with reference data including methicillin resistance in staphylococci (98%), high-level aminoglycoside resistance in enterococci (100%), VanA and VanB phenotypes in enterococci (100%), and ESBLs in Enterobacteriaccae (93.8%). The VITEK 2 AES System appears a reliable tool for the detection and interpretive reading of clinically important mechanisms of resistance and can be recommended for routine work.

2

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Biosurfactant from Lactobacillus sp. as an antibiofilm agent

86%

Kaur S., Kaur R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

3

Resistance of Helicobacter pylori to macrolides and nitroimidazole compounds. The current situation

86%

Megraud F., Occhialini A., Doermann H. P.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

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nr 4

4

Influence of piperidine chlorides derivatives on thermotropic behaviour of phosphatidylcholine and phosphatidylcholine-cholesterol bilayers

86%

Rozycka-Roszak B., Wozniak E., Dega-Szafran Z.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

5

Cathelicidin LL-37: a multitask antimicrobial peptide

72%

Bucki R., Leszczynska K., Namiot A., Sokolowski W.

Archivum Immunologiae et Therapiae Experimentalis

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2010

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tom 58

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nr 1

6

Prevalence of Helicobacter pullorum in Egyptian hen’s eggs and in vitro susceptibility to different antimicrobial agents

72%

Hameed K. G. A., Sender G.

Animal Science Papers and Reports

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2011

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tom 29

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nr 3

The objective of the study was to investigate the occurrence of H. pullorum in chicken hens’ eggs and to determine their in vitro susceptibility to various antimicrobial agents. A total of 300 fresh commercial chicken eggs of Balady native breed and poultry farms (PF) were collected from both Assiut and Qena Governorates in Egypt. Every five eggs represented one sample (mixed contents of five eggs). The eggshell and eggshell contents were examined for the presence of Helicobacter species by conventional methods, next confirmed with PCR. The highest incidence of contamination with Helicobacter species was recorded in eggs from Assiut Governorate poultry farms. The obtained results of Helicobacter pullorum (H. pullorum) isolation revealed that poultry farms egg shells and contents were less contaminated in both Governorates than Balady ones. Additionally, fresh egg contents of poultry farms in both Governorates were free from H. pullorum. All 12 isolates that were conventionally classified as carrying H. pullorum occurred free from the organism when tested with PCR method by detection of one PCR product on agarose gel that matched the predicted size of 477 bp that corresponding to 16S rRNA region of the gene. H. pullorum show in vitro susceptibility to almost all tested types of antibiotics except for ampicillin, ceftriaxone, and sulphamethoxazole trimethoprim.

7

Effect of increasing doses of enrofloxacin on chicken articular cartilage

72%

Maslanka T., Jaroszewski J. J., Mikolajczyk A., Rotkiewicz T.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 1

Enrofloxacin is a synthetic chemotherapeutic agent from the class of the fluoroquinolones that is widely used to treat bacterial infections in animals. Fluoroquinolones cause severe lesions in articular-epiphyseal cartilage complexes of growing mammals. The aim of the present study was to determine whether enrofloxacin has chondrotoxic, dose- and time-dependent effects on avian articular cartilage. 21-day-old male broiler chickens were treated orally with a single or five doses of 10, 50, 100, 300 and 600 mg/kg/day of enrofloxacin. 24 hours after the last dose the animals were killed and femoral head with condyles and tibial condyles were subject to a gross and histopathological investigation. The lesion scoring system was used to determine the progression of lesions. The mean score in birds treated with a single dose of 300 and 600 mg/kg of enrofloxacin was significantly increased when compared to the control group, while the administration of one dose of 10, 50 and 100 mg/kg of the drug did not cause substantial changes in the examined articular cartilages. The mean score was significantly greater in birds dosed for 5 days with 50, 100, 300 or 600 mg/kg/day of enrofloxacin when compared to the control group. Histologic changes included, among others, occurrence of chondrocytes with shrunken cytoplasm and pyknotic nuclei, spindle-shaped cells, clusters of chondrocytes and loss of proteoglycan. In conclusions, our results indicate that the use of enrofloxacin in growing chickens at recommended dosage is safe from the point of view of possibility of chondrotoxic effect. Only very high dosage of enrofloxacin, significantly exceeding the therapeutically applied doses, can induce toxic effects in articular cartilage and intensity of chondrotoxicity is dose- and time-dependent. Moreover, our findings suggest that quinolone-induced arthropathy is considerably less expressed in birds than in mammals.

8

Analysis of Proteus mirabilis distribution in multi-species biofilms on urinary catheters and determination of bacteria resistance to antimicrobial agents

72%

Moryl M., Torzewska A., Jalmuzna P., Rozalski A.

Polish Journal of Microbiology

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2013

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tom 62

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nr 4

The objectives of the investigation presented in this paper were: to examine the frequency of P. mirabilis isolation from catheters and assess the complexity of multi-species biofilms which these bacteria form, as well as to determine the vulnerability of planktonic and sessile P. mirabilis populations to popular antibiotics and compare it to the susceptibility of other Gram-negative bacteria isolated as associated flora from multi-species biofilm. 88 urological catheters, collected from long-term catheterized patients were examined. Uropathogens were recovered from the catheter surface by sonication, and identified on standard diagnostic media. The broth-microdilution method and the MBEC High-throughput Screening assay were used to determine the bacterial resistance to antibiotics. 279 microorganisms were isolated from 88 urinary catheter biofilms. The Enterobacteriaceae family were the most frequently detected bacteria (53.2% of isolates), whereas Proteus spp. isolation accounted for 17.9%, which placed these bacilli on the third position in the Enterobacteraceae family. Among all the tested drugs, amikacin and cephalosporins (ceftriaxone, cefotaxime and cefaclor) exhibited the highest activity against P. mirabilis planktonic cells, 86% and 73% of strains were susceptible to these antibiotics, respectively. 100% of P. mirabilis sessile forms were resistant to cefepime, ciprofloxacin, gatifloxacin, and norfloxacin. Amikacin and ceftriaxone affected only 5% of sessile forms. The planktonic cells of the other studied uropathogens were mostly vulnerable to the all tested drugs (exception P. aeruginosa strains), the most effective of which occurred to be amikacin and cefepime. Obtained MBECs values were 2-512-fold higher than MICs assessed for planktonic forms.

9

Cost-effectiveness of H. pylori eradication

72%

Roesch W.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 1

10

New plant glycoprotein against methicillin resistant staphylococci and enterococci

72%

Fik E., Gozdzicka-Jozefiak A., Haertle T., Mirska I., Kedzia W.

Acta Microbiologica Polonica

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1997

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tom 46

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nr 3

11

The phenotypic and genotypic characteristics of antibiotic resistance in Escherichia coli populations isolated from farm animals with different exposure to antimicrobial agents

58%

Mazurek J., Pusz P., Bok E., Stosik M., Baldy-Chudzik K.

Polish Journal of Microbiology

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2013

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tom 62

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nr 2

The aim of the study was to determine the influence of the presence or the absence of antibiotic input on the emergence and maintenance of resistance in commensal bacteria from food producing animals. The research material constituted E.coli isolates from two animal species: swine at different age from one conventional pig farm with antibiotic input in young pigs and from beef and dairy cattle originated from organic breeding farm. The sensitivity to 16 antimicrobial agents was tested, and the presence of 15 resistance genes was examined. In E.coli from swine, the most prevalent resistance was resistance to streptomycin (88.3%), co-trimoxazole (78.8%), tetracycline (57.3%) ampicillin (49.3%) and doxycycline (44.9%) with multiple resistance in the majority. The most commonly observed resistance genes were: blaTEM (45.2%), tetA (35.8%), aadA1 (35.0%), sul3 (29.5%), dfrA1 (20.4%). Differences in phenotypes and genotypes of E.coli between young swine undergoing prevention program and the older ones without the antibiotic pressure occurred. A disparate resistance was found in E.coli from cattle: cephalothin (36.9%), cefuroxime (18.9%), doxycycline (8.2%), nitrofurantoin (7.7%), and concerned mainly dairy cows. Among isolates from cattle, multidrug resistance was outnumbered by resistance to one or two antibiotics and the only found gene markers were: blaSHV (3.4%), tetA (1.29%), blaTEM (0.43%) and tetC (0.43%). The presented outcomes provide evidence that antimicrobial pressure contributes to resistance development, and enteric microflora constitutes an essential reservoir of resistance genes.

12

Resistance mechanisms of Acinetobacter spp. to beta-lactam antibiotics, aminoglycosides and quinolones

58%

Karakulska J., Kaczynska M., Pobucewicz A., Nawrotek P., Ferlas M., Czernomysy-Furowicz D.

Advances in Agricultural Sciences

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2010

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tom 13

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nr 1

13

Phytochemical and bioactivity diversity in the extracts from bulbs and leaves of different populations of Allium jesdianum, a valuable underutilized vegetable

58%

Ghasemi Pirbalouti A.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 2

Allium jesdianum Boiss. & Buhse (Yazdi onion) belonging the family Alliaceae, is an endemic species of Iran that grows wild in the Zagros Mountains range, southwestern Iran. The indigenous people of Iran use the leaves and bulbs of A. jesdianum for the treatment of colds and kidney problems. The bulbs and leaves of various populations of the plant were collected from the alpine regions in Chaharmahal va Bakhtiari province, southwestern Iran. The total phenolic content of ethanol extract was determined by FolinCiocalteu method, the antioxidant activity was evaluated measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the antibacterial activity of the extracts against four bacteria was determined by serial dilution assay. Results indicated that the total phenolic content in ethanol extracts from leaves and bulbs of A. jesdianum ranged between 27.83 to 98.23 mg GAE/g extract. A comparison of all plant extracts in the DPPH assay indicated that ethanol extracts from the populations of A. jesdianum leaves were the most effective free radical scavenging agents. The extracts indicated moderate-to-good inhibitory activities against four bacteria, especially against B. cereus. This finding suggests that the bulbs and leaves of A. jesdianum may be considered a natural source of antioxidants and antimicrobial agents.

14

Virulence factor genotypes of Helicobacter pylori affect cure rates of eradication therapy

58%

Sugimoto M., Yamaoka Y.

Archivum Immunologiae et Therapiae Experimentalis

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2009

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tom 57

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nr 1

45-56

15

A study comparing the efficacy of antimicrobial agents versus enzyme (P-gp) inducers in the treatment of 2,4,6 trinitrobenzenesulfonic acid-induced colitis in rats

58%

Toklu H. Z., Kabasakal L., Imeryuz N., Kan B., Celikel C., Cetinel S., Orun O., Yuksel M., Dulger G. A.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 4

16

Resistance to antimicrobial agents of Campylobacter spp. strains isolated from animals in Poland

58%

Krutkiewicz A., Salamaszynska-Guz A., Rzewuska M., Klimuszko D., Binek M.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 4

A total of 69 Campylobacter jejuni and 16 Campylobacter coli strains isolated from chicken, dog and pig stool samples were characterized based on their resistance to five antimicrobial agents and on plasmid pTet profiles. Antimicrobials used in this study were: amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, tetracycline and trimethoprim/sulfamethoxazole. Among the isolates studied, 91.7% were resistant to one or more antimicrobial agent. The highest level of resistance for the whole test group was to trimethoprim/sulfamethoxazole (57.6%), followed by ciprofloxacin (44.2%) and tetracycline (20%). All isolates were susceptible to amoxicillin/clavulanic acid. Strains isolated from chickens were susceptible to erythromycin. Few erythromycin-resistant strains were isolated from dogs and pigs (5.8%). C. coli strains exhibited a higher antibiotic resistance than C. jejuni strains, excluding resistance to trimethoprim/sulfamethoxazole. The pTet plasmid harboring the tet(O) gene was detected in 14 Campylobacter spp. strains. Our studies demonstrate that the majority (71.4%) of tetracycline-resistant isolates carry a plasmid-borne tet(O) gene, particularly strains for which the minimum inhibitory concentration (MIC) are ≥ 256 μg/ml. In conclusion, we have found high-level trimethoprim/sulfamethoxazole, ciprofloxacin and tetracycline resistance in Polish strains isolated from different sources. This study has demonstrated that resistance of Campylobacter species differs depending on both the bacterial species and animal origins. All strains that displayed resistance to four antimicrobial agents were isolated from pigs. Localization of the tet(O) gene on either plasmid or chromosome was not found to be correlated with tetracycline resistance.

17

Anaerobic bacteria colonizing the lower airways in lung cancer patients

58%

Rybojad P., Los R., Sawicki M., Tabarkiewicz J., Malm A.

Folia Histochemica et Cytobiologica

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2011

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tom 49

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nr 2

18

New antibacterial therapeutics and strategies

58%

Kurek A., Grudniak A., Kraczkiewicz-Dowjat A., Wolska K.

Polish Journal of Microbiology

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2011

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tom 60

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nr 1

Studies on new antibacterial therapeutics and strategies are currently being conducted in many microbiological, pharmaceutical and biochemical laboratories. The antibacterial activity of plant-derived compounds as well as silver and gold nanoparticles is the subject of this minireview. The application of photodynamic therapy is also discussed.

19

Phytochemical evaluation and comparative antibiocide efficacy of Aqueous, Ethanolic and equal mixture of aqueous and ethanolic (1:1) bark extract of Lannea coromandelica L. procured from Eastern region of India

58%

Das K.

International Letters of Natural Sciences

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2014

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tom 21

The present investigation was evaluating the potential antibacterial activity of three different extracts of the bark of Lannea coromandelica Linn. (LC) tree procured from Eastern India. Extraction of bark separation was carried out using aqueous, ethanol and a mixture of aqueous and ethanol. Microbiocides of all the extracts were separately evaluated against several microorganisms viz. Bacillus substilis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonus aeruginosa, Escherichia coli and Serratia marcescens by agar diffusion technique. The Minimum Inhibition Concentration (MIC) of all the extracts was carried out by the serial dilution method. The results of MIC ranged from 12.5 to 150 mg/ml (all the three extracts). The concentration dependent (**P < 0.01) potential antimicrobial activity was resulted and at the dose of 200 mg/ml, combined aqueous and ethanol extract of LC (LCAE + LCEE) gave significant results against gram positive bacteria where the maximum zone of inhibition was recorded against Streptococcus pyogenes (17.0± 0.05**) followed by Straphyloccus aureus (13.6 ±0.05**). Further, the same extract showed the maximum relative percentage inhibition against Straphyloccus aureus (178.64%) followed by Streptococcus pyogenes (143.42%). Such variation may be due to the effects of choice of solvent and the quantity of the extracted amount and also the geographical source of the plant part. These results represent scientific evidence to support the traditional medicinal uses of LC bark extracts and indicate a promising potential used against the treatment of infectious diseases caused by pathogenic bacteria and also provide scientific evidence for their efficacy to prepare the alternate newer medicine for antibiotics.

20

Antimicrobial properties of 6-bromoeugenol and eugenol

58%

Mahboub R., Memmou F.

International Letters of Natural Sciences

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2016

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tom 53

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains: Pseudomonas aeruginosa (S1), Escherichia coli (S2) and Staphylococcus aureus (S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with the Pseudomonas aeruginosa (S1) at all concentrations and with the Escherichia coli (S2) at 0.5 mg/ml. We remark that the Pseudomonas aeruginosa (S1) is the more sensitive strain than Escherichia coli (S2) and Staphylococcus aureus (S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

Ograniczanie wyników

Susceptibility testing and resistance phenotypes detection in bacterial pathogens using the VITEK 2 system

Biosurfactant from Lactobacillus sp. as an antibiofilm agent

Resistance of Helicobacter pylori to macrolides and nitroimidazole compounds. The current situation

Influence of piperidine chlorides derivatives on thermotropic behaviour of phosphatidylcholine and phosphatidylcholine-cholesterol bilayers

Cathelicidin LL-37: a multitask antimicrobial peptide

Prevalence of Helicobacter pullorum in Egyptian hen’s eggs and in vitro susceptibility to different antimicrobial agents

Effect of increasing doses of enrofloxacin on chicken articular cartilage

Analysis of Proteus mirabilis distribution in multi-species biofilms on urinary catheters and determination of bacteria resistance to antimicrobial agents

Cost-effectiveness of H. pylori eradication

New plant glycoprotein against methicillin resistant staphylococci and enterococci

The phenotypic and genotypic characteristics of antibiotic resistance in Escherichia coli populations isolated from farm animals with different exposure to antimicrobial agents

Resistance mechanisms of Acinetobacter spp. to beta-lactam antibiotics, aminoglycosides and quinolones

Phytochemical and bioactivity diversity in the extracts from bulbs and leaves of different populations of Allium jesdianum, a valuable underutilized vegetable

Virulence factor genotypes of Helicobacter pylori affect cure rates of eradication therapy

A study comparing the efficacy of antimicrobial agents versus enzyme (P-gp) inducers in the treatment of 2,4,6 trinitrobenzenesulfonic acid-induced colitis in rats