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Production of haploids in ancient, local and modern wheat by anther culture and maize pollination

100%

Gurtay G., Kutlu I., Avci S.

Acta Biologica Cracoviensia. Series Botanica

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2021

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tom 63

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nr 1

2

Anther culture and identification of different Polish wheat cultivars

100%

Czaplicki A. Z., Borsuk P., Batko A.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 2

3

Induced androgenesis of Capsicum frutescens L.

100%

Nowaczyk P., Kisiala A., Olszewska D.

Acta Physiologiae Plantarum

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2006

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tom 28

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nr 1

The aim of the research was to make a preliminary determination of the effectiveness of the in duc tion of haploids in Capsicum frutescens L. In order to induce androgenesis red and yellow fruit forms of species were used, each bred by the re searchers on their own. The experiment was per formed in October. An ther cultures were conducted according to a modified me - thod developed by Dumas et al. (1981) for C. annuum L. The anthers were laid on CP medium con taining 0.01 mg·dm⁻³ 2.4-D and 0.01 mg·dm⁻³ kinetin, with the addition of 0.5 g·dm⁻³ of activated carbon and 5 mg×dm⁻³ of silvernitrate, solidified with 8 g·dm⁻³ of agar. The cul tures were in cu bated in the dark at 35 deg C for 8 days. Next they were trans ferred to 25 deg C under a 12-hour photoperiod. After 14 days of induction, anthers were trans ferred to R₁ medium supplemented with 0.1 mg·dm⁻³ kinetin. Obtained embryos were subsequently transplanted onto V₃ hormone-free me dium and well growing plants were planted in green houses. The efficiency of androgenesis for both C. frutescens L. forms was relatively low and it did not exceed 5 %. The ploidy level of the result ing plants was determined by flow-cytometric analysis. The regenerants con sisted of about equal numbers of haploids and diploids. Additionally, among plants regenerated from anthers of yellow fruit forms, two mixoploids were observed.

4

Combining abilities of anther culture response in hexaploid triticale

100%

Slusarkiewicz-Jarzina A., Ponitka A., Adamski T., Surma M.

Journal of Applied Genetics

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1996

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tom 37A

5

Hybrids of Capsicum frutescens L. x Capsicum annuum L. as a material for androgenic haploids production

86%

Niklas-Nowak A., Nowaczyk P.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

6

Reproductive properties of Capsicum spp. androdiploids derived from in vitro anther culture

86%

Nowaczyk L., Nowaczyk P.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

7

Evaluation of the regeneration ability of Brassica interspecific hybrids in in vitro anther culture

86%

Wojciechowski A., Niemann J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

8

RAPD technique application for intraline evaluation of androgenic carrot plants

86%

Staniaszek M., Habdas H.

Folia Horticulturae

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2006

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tom 18

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nr 2

9

Comparison of the efficiency of haploid and doubled haploid induction in anther cultures of winter and spring forms of triticale (× Triticosecale Wittm.)

86%

Slusarkiewicz-Jarzina A., Ponitka A., Pudelska H., Wozna J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

10

The androgenic response in anther culture of spring wheat is greater in genotypes with solid stem in contrast to genotypes with hollow stem

86%

Weigt D., Kiel A., Nawracala J., Pluta M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

11

Genetic and nongenetic factors influencing callus induction in Miscanthus sinensis [Anderss.] anther cultures

86%

Glowacka K., Jezowski S.

Journal of Applied Genetics

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2009

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tom 50

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nr 4

341-345

Miscanthus sinensis is a promising species for biomass production. Influences of genetic and nongenetic factors on androgenesis induction efficiency were investigated. This is the first report on successful induction of pollen-derived callus in M. sinensis. The callus yield was strongly affected by genotype. A beneficial influence of cold pretreatment of spikes on androgenesis induction was observed. The highest yield of calli was obtained in cultures on a modified C17 medium. The results suggest that the high callus yield might be caused by the late culture initiation. The beginning of anther culture at the end of the flowering season caused a 17-fold increase in callus yield, in comparison to culture initiated at the height of the flowering season (August). It is likely, however, that the efficiency of androgenesis induction in the case of M. sinensis anther culture beginning in October could be related to a positive influence of growing donor plants in conditions of cooler and shorter day, i.e. 11-h day with temperature around 11°C and 13-h night with temperature around 5°C. Results of this study can significantly support the development of effective methods of M. sinensis haploidization, which could be used in crop improvement by breeding.

12

Effect of donor plant treatment with 2,4-dichlorophenoxyacetic acid on the anther culture of hot Capsicum spp.

86%

Nowaczyk L., Nowaczyk P., Olszewska D., Niklas-Nowak A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

13

High frequency of doubled haploid plant production in spelt wheat

86%

Lantos C., Jenes B., Bona L., Cserhati M., Pauk J.

Acta Biologica Cracoviensia. Series Botanica

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2016

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tom 58

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nr 2

This is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing importance not only in applied research but also in organic farming and changing nutritional standards. In this study, an efficient AC protocol has been described for ‘GK Fehér’ spelt wheat. The number of AC-derived embryolike structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per 100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of ‘GK Fehér’ 306 green plantlets were produced in vitro and 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while a few seeds were harvested from seven partially fertile plants.

14

Factors influencing the efficiency of wheat anther culture

86%

Lantos C., Pauk J.

Acta Biologica Cracoviensia. Series Botanica

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2020

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tom 62

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nr 2

15

Doubled haploids in production of male sterility maintaining triticale [Triticosecale Wittmack] lines

86%

Warzecha R., Sowa S., Salak-Warzecha K., Oleszczuk S., Sliwinska E., Zimny J.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 2

The androgenetic response of several selected male sterilitymaintainer genotypes of triticale was investigated. Androgenesis induction was obtained in all cultivars, but a large genotypic variation in green plant regeneration was observed. The number of regenerated triticale plants varied from 0.1 to 4.7 green plants per spike, depending on genotype. Spontaneous doubling of chromosomes varied from 14 to 60 % for particular genotypes and, on average, reached the value of 34 % for all genotypes. Fertile DH lines obtained in this study will find practical application in the development of triticale male sterile lines that are desirable in hybrid breeding.

16

Anther cultures of carrot [Daucus carota L.]

86%

Adamus A., Michalik B.

Folia Horticulturae

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2003

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tom 15

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nr 2

17

Anther culture and identification of different Polish wheat cultivars

86%

Czaplicki A. Z., Borsuk P., Batko A.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

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1997

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tom 39

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nr 1

18

Wheat anther culture as affected by various cultural changes and supplements

86%

Simonson R. L., Baenziger P. S., Gustafson V. D.

Journal of Applied Genetics

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1997

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tom 38

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nr 4

With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.

19

Using enzyme polymorphism to identify the gametic origin of flax regenerants

86%

Bartosova Z., Obert B., Takac T., Kormutak A., Pretova A.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

Anther culture is currently the most successful method for production of doubled haploid lines in flax. Recently, ovary culture was also described as a good source of doubled haploids. In this contribution we investigated the incorporation of enzyme polymorphism of acid phosphatase and peroxidase as molecular markers for the gametic origin of flax plants derived from anther and ovary cultures.

20

Cytogenetic status of barley plants regenerated from anther culture

86%

Szarejko I., Janusz A., Nabialkowska D., Trojanowska A.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

Ograniczanie wyników

Production of haploids in ancient, local and modern wheat by anther culture and maize pollination

Anther culture and identification of different Polish wheat cultivars

Induced androgenesis of Capsicum frutescens L.

Combining abilities of anther culture response in hexaploid triticale

Hybrids of Capsicum frutescens L. x Capsicum annuum L. as a material for androgenic haploids production

Reproductive properties of Capsicum spp. androdiploids derived from in vitro anther culture

Evaluation of the regeneration ability of Brassica interspecific hybrids in in vitro anther culture

RAPD technique application for intraline evaluation of androgenic carrot plants

Comparison of the efficiency of haploid and doubled haploid induction in anther cultures of winter and spring forms of triticale (× Triticosecale Wittm.)

The androgenic response in anther culture of spring wheat is greater in genotypes with solid stem in contrast to genotypes with hollow stem