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The experimental materials comprised 400 Ross 308 chickens (200 ♂ and 200 ♀), divided randomly into four groups kept in 16 pens, as follows: a negative control group (I) – a diet without additives, a positive control group (II) – a diet supplemented with a combination of the antibiotic growth promoter flavomycin (10 mg kg⁻¹ feed) and the coccidiostat robenidine (500 mg kg⁻¹ starter and grower feed), and two experimental groups – diets supplemented with chemically standardized propolis – 10 mg kg⁻¹ feed in group 3 and 50 mg kg⁻¹ feed in group IV. The body weights of chickens were determined once a week. Feed intake, mortality and culling rates were monitored regularly. At the end of the experiment, on day 42, 12 chickens (6 ♂ and 6 ♀) were selected from each group for slaughter and carcass quality analysis. The final body weights of chickens, feed intake (kg) per kg body weight and carcass weight during the rearing period did not differ significantly between groups. The lowest feed intake per kg lean meat was noted in chickens fed a diet with 50 mg propolis/kg feed (3.457 kg), and the highest – in broilers fed a diet with 10 mg propolis/kg feed (3.611 kg). The weight of carcass and selected carcass parts (neck, breast, legs) tended to increase in chickens that received propolis, although the noted differences were statistically non-significant. The percentage content of legs in the carcass was significantly lower in chickens fed a diet with an antibiotic growth promoter (AGP). The legs of chickens fed 50 mg propolis had a significantly higher muscle content and a lower fat content, compared with the legs of birds fed a diet with AGP.
Two experiments were conducted to determine optimal standardized ileal digestible (SID) tryptophan:lysine ratio for piglets using growth performance and plasma urea N as response criteria. In Experiment 1, piglets were fed 6 diets with 8.5, 9.5, 10.5, 11.5, 12.5 or 13.5 g · kg–1 SID lysine to estimate lysine requirement. In Experiment 2, piglets were fed 6 diets with 9.2 g · kg–1 SID lysine, and 0.178, 0.182, 0.196, 0.216, 0.227 or 0.236 SID trytophan:lysine to estimate optimal tryptophan:lysine ratio. The SID lysine requirement was estimated to be 10.2 g · kg–1. Response criteria measured were unaffected by SID tryptophan:lysine ratio, implying that the diets fed were not deficient in tryptophan. In conclusion, the SID lysine requirement for the piglets was close to the recommendation by NRC (1998; 10.1 g · kg–1), but not NRC (2012; 12.3 g · kg–1). The optimal SID tryptophan:lysine ratio of the piglets was either equal to or less than 0.178.
Effect of herbal mixture in beef cattle diets on fattening performance and nutrient digestibility. The aim of the study was to evaluate the impact of herbal mixtureaddition in beef cattle diet on daily weight gain, feed utilization and the nutrient digestibility. The study was performed on 16 Limousin bulls during the fattening period from 100 kg to 650 kg of body weight. The animals were divided into two groups: a control (C) and experimental (E) group. All experimental bulls received the same daily rations composed of basal feeds and a concentrate mixture. The experimental factor was a certified mixture of dried chopped herbs (the author's own elaboration): Echinacea (Echinacea purpurea Moench.), garlic (Allium sativum L.), thyme (Thymus vulgaris), caraway (Carum carvi), and liquorice(Glycyrrhiza glabra), given additionally to the rations, in the amount of 3% of the predicted daily dry matter intake. During the study, the feed intake, animal weight gain, feed utilization per 1 kg of body weight gain, and nutrient digestibility were determined.The digestibility study was performed with the indicator method using acid-insoluble ash (AIA) as internal indicator. The addition of herbs improved animals’ performance. Higher daily gains in the experimental group of animals (1194 g/day/animal), compared to those fed without the addition of herbs (982 g/day/animal), were found with simultaneous better feed utilization for 1kg of growth (5.01 kg in the experimental group, 5.94 kg in the control group). Higher apparent protein and fat digestibility coefficients were noted in the animals from the experimental group (by 1.3 and 2.5 percentage points, respectively), compared to the control ones. In conclusion at least a 3% addition of herbal mixture containing Echinacea, garlic, thyme, caraway,and liquoricecan be recommended in beef cattle diet in order to improve thefattening performance. The addition of herbs improves animals’ performance. Higher daily gains in the experimental group of animals, with simultaneous better utilization of feed per1 kgof growth, were found.
The objective of this study was to evaluate the behaviour of fibre in the digestive tract on the basis of the passage kinetics of forage and concentrate particles in cows fed different omega-6 fatty-acid sources. The scientific hypothesis of this study was that omega-6 fatty acids do not interfere with the digestion of fibre in the diets of dairy cows. Five primiparous lactating Holstein cows were used in the experiment. The experimental diets were: control (C), ground soyabean (GS), cottonseed (CS), soyabean oil (SO), calcium salts of fatty acids (CSFA). The global mean estimates for the parameters of passage rate (γ) were 0.038 and 0.055 h–1 for forage and concentrate, respectively. The only significant effect with respect to the passage rate was a high negative correlation between the concentrate passage rate and dry matter intake. There was less undegradable neutral detergent fibre (NDF) in treatments without added lipid. Dietary supplementation with lipid sources does not alter the kinetic parameters of roughage and concentrate particle passage or in vitro NDF degradation. Sources of omega-6 fatty acids do not alter the rumen degradation and transit of fibre.
Lipid oxidation is the primary cause of deterioration in the quality of frozen meat and meat products. Oxidative deterioration of meat lipids during frozen storage can directly affect the colour, flavour, texture, nutritive value, and safety of food. Natural antioxidants reduce lipid oxidation and as a consequence may improve meat quality. In the present study we investigate the effect of three levels of dietary vitamin E on animal growth performance and on meat oxidation. HPLC analyses were performed in order to assess a-tocopherol levels in blood serum and its deposition in muscles. The oxidative stability of muscle was examined over 7 days of refrigeration storage by means of thiobarbituric acid reactive substances (TBARS). We concluded that supplementation with vitamin E augmented a-tocopherol levels in blood serum and muscles from pig samples receiving 300 mg/kg feed. Moreover lipid oxidation in chilled meat was successfully reduced.
Forty ostriches were raised in five groups [control (C) or with 4% (L4) or 8% (L8) linseed, or 5% (R5) or 10% (R10) rapeseed in the diet]. Linseed supplementation (L4 and L8) improved the nutritive value of the ostrich meat by increasing (P<0.001) the α-linolenic acid content (>4.2%FAtotal) and PUFA/SFA ratio (>1.0) as compared with the control group (1.7% FAtotal and <0.94, α-linolenic acid and PUFA/SFA, respectively), whereas the effect of rapeseed was lower (2.2%, and <0.99, α-linolenic acid and PUFA/SFA, respectively). Dietary treatment lowered (P<0.001) the n-6/n-3 ratio from ca. 11 in the C group to 4 in the L8 and L4 groups. Overall, the results indicate that inclusion of linseed into ostrich diets has a positive effect on the fatty acid composition, allowing the production of meat enriched with n-3 fatty acids
Background. Since arsenic compounds have an affinity to thiol groups their greatest amounts can then be found in the tissues containing sulphur - rich proteins, like beta- keratin in skin, hair and nails. Accumulation of arsenic also depends on the macronutrient content in daily food ration. The deficiency and excess of both the protein and fat may contribute to a higher content of arsenic in the organism, including hair in human or fur in animals. Objective. Hair and fur is a good indicator of population exposure to many toxic substances, including arsenic. The degree of arsenic accumulation may depend on the diet and nutritional status. The aim of this study was to determine the effect of protein and fat in diet on the accumulation of arsenic in rats’ fur. Material and Methods. A total number of 70 male Buffalo rats (body weight 200 - 220 g, age - 6 weeks) were divided into 10 groups. Rats were housed in plastic cages (4 per cage) in a 12h light/dark cycle for 6 weeks. The diets of different protein and fat contents ware administered to the animals. Five of ten groups of rats received throughout the whole period 10 ppm sodium arsenite dissolved in distilled drinking water (about 250 μg As/animal/day). The arsenic were determined with the method of atomic adsorption spectrometry in conjunction with a graphite-furnace atomize using a Varian AA240FS apparatus. Results. The highest arsenic concentrations were found in fur of rats which were given low protein diet and water with arsenic. The lowest arsenic contents were found in fur of rats, which were given control diet and high protein diet with arsenic in water. Conclusions. Balanced control diet or high protein diet protected organism from arsenic accumulation, only small increase of arsenic content in rats’ fur, compared to the control group, was observed.
The stomach content of 74 wild boars harvested in the Zielonka Game Investigation Centre in 2005-2007 were analyzed. According to the hunting management schedule and the cycles of forest and farmland food occurrence, three research seasons were selected and compared with respect to the trophic behaviour of wild boar. As many authors claimed, the animals extraordinarily tended to eat mainly the feed supplied to the forest. Such a disruption in the natural use of the food niche in the forest environment, resulting from an irrational feeding policy, interferes with the natural trophic role of this species.
The influence of dietary conjugated linoleic acid (CLA) isomers (CLAmix) and/or Se as Na2SeO3 (SeIV) or selenized yeast (SeY) on the relative body weight gain (RBWG) of rats, pancreas weight, concentrations of CLA isomers and of other fatty acids (FA) in the pancreas were studied. For 6 weeks, female Wistar rats (aged 8 weeks) were fed ad libitum the Labofeed H diet enriched in 1.5% CLAmix, 0.2 ppm Se (LSe), 0.5 ppm Se (HSe) as SeIV (i.e. LSeIV or HSeIV, respectively), or SeY (i.e. LSeY or HSeY, respectively). Each group contained eight rats. The results documented that dietary LSeIV and CLAmix most effectively increased the RBWG of rats and feed conversion efficiency, while the diet with HSeIV most efficiently increased pancreas weight compared with the control rats. The diets enriched in CLAmix and Se as SeIV or SeY increased the sum of saturated fatty acids (SFA), the atherogenic SFA, A-SFA index, thrombogenic SFA (T-SFA), and the T-SFA index in the pancreas of rats compared with the control group. The diets containing CLAmix and Se as SeIV or SeY stimulated the accumulation of cis9trans11CLA, trans10cis12CLA, as well as the sum of all CLA isomers in the pancreas compared with rats fed the diet enriched in CLAmix. These diets increased the concentration of n-6 polyunsaturated FA (n-6 PUFA) and decreased the level of long-chain n-6 PUFA (Ln-6 PUFA) in the pancreas compared with the control rats and rats fed the diets with SeIV or SeY. The diets containing CLAmix and Se (as SeIV or SeY) increased the concentration of n-3 PUFA and Ln-3 PUFA in the pancreas compared with the control rats. Our study findings documenting that dietary Se and/or CLAmix and Se (as SeIV or SeY) considerably increased the concentration of PUFA, especially n-3 PUFA, improved the n-6 PUFA/n-3 PUFA ratio in the pancreas are valuable information for scientists carrying out research to improve animal and human health.
A total of 150 one-day-old male broiler chicks (Ross 308) and 120 one-day-old female Muscovy ducklings were distributed over 15 and 12 pens, respectively. All birds received the same diet during the first period of life. Throughout the second period (36-56 days for broiler chickens and 43-69 days for Muscovy ducks) different source plant extracts were supplemented to the basal diet for each species; dietary treatments were assigned to three pens each. In the chicken (CK) trial the following dry extracts were tested: tomato (Solanum lycopersicum) skin (200 mg lycopene kg-1 feed; CK-L200 group), orange (Citrus aurantium) peel (200 mg hesperidin kg-1 feed; CK-O200 group), and green tea (Camellia sinensis) leaves (200 mg catechins kg-1 feed; CK-T200 group). For the Muscovy duck (DK) trial the tested extracts were produced from rosemary (Rosmarinus officinalis) leaves (200 mg carnosic acid kg-1 feed; DK-R200 group) and orange (Citrus aurantium) peel (200 mg hesperidin kg-1 feed; DK-O200 group). The effects in both species were compared with those for the unsupplemented diet (CK-C and DK-C) and the diet supplemented with 200 mg of alpha-tocopheryl acetate (CK-E200 and DK-E200). At the end of each trial three birds per pen were slaughtered. Growth performance,pH and meat proximate composition in both species were not affected by dietary treatments. The TBARS value of chicken leg meat from the unsupplemented group was 3.86, while on average in CK-E200, CK-L200 and CK-O200 it was by 60, 55 and 63% lower (P<0.05), whereas in CK-T200 it was by 25% higher (P<0.05). Dietary treatments did not exert any antioxidant effects on chicken breast meat. The TBARS value of duck breast meat and leg meat from the control was 1.39 and 4.51,respectively, while on average in the DK-E200, DK-O200 and DK-R200 groups it was by 82 and 71%, 33 and 46%, and 66 and 47% lower (P<0.05), respectively. The magnitude of the antioxidant action of vegetable dry extract in this trial was lower than that of alpha-tocopheryl acetate.
The aim of the study was to investigate the effect of different levels of protein in a diet on the incorporation of endogenous urea nitrogen (EUN) into individual amino acids (AA) of the ruminal bacteria of goats fed a low- (LP), medium- (MP), or high-protein diet (HP) in a 3 × 3 Latin square design. Three Alpine goats of about 35 kg body weight fitted with cannula into the rumen and catheter into the jugular vein were fed three isoenergetic diets containing 11% (LP), 13% (MP), or 16% (HP) crude protein in dry matter. The goats were infused for 6 days continuously with an 15N urea solution into the jugular vein. Ruminal bacteria were hydrolysed with 6M HCl. Next, butyl derivatives of free bacterial AA were obtained using HCl in butanol, then N-acylated using trifluoroacetic acid anhydride and analysed by gas chromatography using a mass-selective detector. The concentration of urea in plasma was 178, 356 and 667 mg · l–1 in goats from groups LP, MP and HP, respectively. 15N-excess during the infusion of labelled urea was significantly higher (P < 0.05) in the vast majority of AA of ruminal bacteria from goats fed the LP diet in comparison with goats fed the HP diet. Therefore, the level of protein in the diets affects the incorporation of EUN into bacterial AA. With the LP diet, EUN was incorporated mostly into glutamic acid, isoleucine and arginine, while in the case of the HP diet, into glutamic acid and arginine, as well as methionine. Regardless of the level of nitrogen in the diets, the incorporation of 15N into proline was very low. Irrespective of the dietary nitrogen level, EUN appears to be predominantly used for synthesis of glutamic acid in ruminal bacteria.
Diets supplemented with Mytilus galloprovincialis from polluted and non-polluted waters and their influence on zinc content in liver of rats loaded with cholesterol. Mussels may reflect the environmental problems that the ecosystem faces. Mytilus galloprovincialis is widely used as an indicator of water pollution in biological monitoring studies. These mussels are filter feeders and may accumulate important ecosystem pollutants what in turn may pose a potential risk to other organisms in the food chain. The aim of the study was to determine zinc content in the diets supplemented with mussels from polluted and non-polluted areas and in the livers of rats fed these diets for 30 days. The zinc content in the diets with mussels from contaminated and noncontaminated waters and in rat livers was examined using a flame atomic absorption spectrophotometry (Perkin-Elmer 1100B). The slight increase in zinc content from 73.2 ±1.2 mg/ /kg in ch diet (control with 1% of cholesterol) to 77.2 ±0.5 mg/kg in the diet with mussels from polluted site was found, but zinc content in all diet was within the normal limits reviewed by McDowell (1992) – 40 to 100 ppm. Zinc content in the diet supplemented with mussels from nonpolluted site (73.7 ±3.7 mg/kg) was similar to that found in ch diet. It can be concluded that cholesterol does not affect zinc bioaccumulation. However, prolonged consumption of mussels from contaminated sites may increase accumulation of zinc in the liver of rats.
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