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  1. 11 Acta Theriologica

  2. 4 Folia Biologica

  3. 4 Polskie Archiwum Hydrobiologii

  4. 3 Acta Societatis Botanicorum Poloniae

  5. 3 Acta Theriologica. Supplement

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  1. 3 Brzuzan P.

  2. 3 Hartl G. B.

  3. 3 Krzakowa M.

  4. 3 Lewandowski A.

  5. 3 Suchentrunk F.

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  1. 2 2011

  2. 3 2010

  3. 2 2008

  4. 1 2006

  5. 1 2005

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1

Genetic structure of ash-tree [Fraxinus excelsior L.] populations as revealed by peroxidase allozymes

100%
Krzakowa M.
Rocznik Dendrologiczny
|
2001
|
tom 49
2

Allozyme genetic distances and evolutionary relationships in marsupials of North and South America

100%
Barrantes G. E., Daleffe L.
Acta Theriologica
|
1999
|
tom 44
|
nr 3
AUozyme genetic distances and variability were studied by horizontal starch gel electrophoresis in 6 species of marsupials from North and South America representing 4 different genera. Twenty-one presumptive loci were assessed in a total of 151 specimens. Only 1 of 21 loci was found to be monomorphic in the whole sample. Phenetic and cladistic interspecific analysis coincided in rendering two sharply dif­ferentiated subgroups: one comprising species of the genus Didelphis Linnaeus, 1758 CD. marsupialis Linnaeus, 1758, D. virginiana Kerr, 1792 and D. albiventris Lund, 1840), and the other comprising Monodelphis dimidiata fWagner, 1847}, Lestodelphys halli (Thomas, 1921) and Lutreolina crassicaudata (Desmarest, 1804). No relationships between the bradytelic condition, the karyotype stability of this group, and genetic variability were found. On the other hand, the existence of species with brief life span such as Lestodelphys halli and Monodelphis dimidiata (Marmosini tribe) and species with long life span (Didelphini tribe) allowed us to test the hypothesis which correlates generation-time with genetic variability. We conclude that a general explanation for genetic variability must involve more than just generation-time,
3

Genetic variability in goitred gazelle Gazella subgutturosa subgutturosa: allozymes and karyotypes

100%
Schreiber A., Von Hegel G.
Acta Theriologica
|
1999
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tom 44
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nr 1
The electrophoretic polymorphism of blood proteins, and karyotypes, were studied in up to 33 captive-bred Persian goitred gazelles Gazella s. subgutturosa (Giildenstaedt, 1780). Allozymes, haemoglobins and serum proteins representing 33 putative genetic loci displayed four biallelic polymorphisms (carbonic anhydrase, malate dehydrogenase, mannose phosphate isomerase, transferrin), resulting in a percentage polymorphism of p = 0.121, and an expected heterozygosity of He = 0.047. Six males had 2n = 31, and seven females 2n = 30 chromosomes. This sex-specific difference was due to an X-autosomal translocation, coupled with a XYiYz sex determining system in males. Neither karyotypes nor protein polymorphism provided evidence to explain the high mortality of newborn goitred gazelles.
4

Genetic characterisation of a domestic dog canis familiaris breed endemic to South African rural areas

100%
Greyling L. M., Grobler P. J., Van Der Bank H. F., Kotze A.
Acta Theriologica
|
2004
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tom 49
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nr 3
Allozyme electrophoresis (horizontal starch gel and PAGE) and histochemical staining techniques were used to study the genetic composition of an endemic southern African domestic dogCanis familiaris Linnaeus, 1758, the Africanis breed. Genetic differentiation was analysed at 21 protein-coding loci. The results were compared to those for three other populations/breeds: representatives of established Western breeds, crossbred dogs of Western descent from rural areas in South Africa, and indigenous Saluki dogs from the Middle East. Nine polymorphic loci were found (Ak-1,-2, Ck, Per, Hb, Po-A-1 to-3 andPo-Tf). Two unique alleles at the Ck and Po-A-2 loci separated the Africanis breed from the other groups. There were also significant differences between Africanis and the other breeds in pair-wise comparisons of allelic frequencies at polymorphic loci. An assignment test, fixation index values, gene flow and genetic distance values indicated a closer genetic association between the Africanis and Saluki breeds than with dogs of Western origin. This finding supports archaeological evidence that the endemic Africanis breed was introduced from the Middle East into Africa thousands of years ago, and not through later western influences. The average heterozygosity ranged from 0.106–0.15, with least heterozygosity in the Africanis and most in the rural crossbred group. The percentage of polymorphic loci, the mean number of alleles per locus (biologically more significant than heterozygosity), and conformation of genotypes to Hardy-Weinberg proportions showed no evidence of recent loss of genetic diversity in Africanis. Genetic differentiation and support of archaeological evidence by genetics indicate that the endemic southern African domestic dog breed is unique.
5

Genetic differences among several species of Tricladida from the relict Lake Ohrid as revealed by enzyme electrophoresis

100%
Sywula T., Krstanovski Z., Tasevska O., Sell J., Kretowicz T.
Folia Biologica
|
2003
|
tom 51
|
nr 1-2
6

The genus Isotomurus: where molecular markers help to evaluate the importance of morphological characters for the diagnosis of species

100%
Frati F., Carapelli A., Fanciulli P. P., Dallai R.
Polskie Pismo Entomologiczne
|
1995
|
tom 64
|
nr 1-4
7

Inheritance and linkage of allozymes in Juniperus phoenicea L. [Cupressaceae]

100%
Lewandowski A., Samocko J., Boratynski A., Mejnartowicz L.
Acta Societatis Botanicorum Poloniae
|
2000
|
tom 69
|
nr 3
8

Defining new Naegleria spp.using ribosomal DNA sequences

100%
De Jonckheere J. F., Brown S.
Acta Protozoologica
|
1997
|
tom 36
|
nr 4
9

Biochemical-genetic approach towards the systematics of lechwe Kobus leche

100%
De M. D., Apollonio M., Hartl G. B.
Acta Theriologica
|
1995
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tom 40
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nr 3
Red lechwe Kobus leche leche Gray, 1850 (n = 3), black lechwe K. I. smithermani Lydekker, 1900 (n = 10) and Kafue lechwe K. I. kafuensis Haltenorth, 1963 (n = 19) from Zambia were examined for genetic variability and differentiation at 30 pre­sumptive structural loci using horizontal starch gel electrophoresis. Values of polymor­phism (P = 10.0-16.7%) and average heterozygosity (H = 6.3-7.9%) were within the range commonly found in ungulates. Genetic variability was lowest in the red lechwe, which may be due to a genetic bottleneck the Zambia population experienced some 50 years ago. Relative (fst = 21%) and absolute (Nei's 1978, d = 0.020-0.023) genetic differentiation were in accordance with the subspecies status proposed for red lechwe, black lechwe, and Kafue lechwe on the basis of morphological characters.
10

Genetic variability of Dreissena polymorpha [Pallas] populations from Western Pomerania, Poland

100%
Zielinski R., Soroka M., Polok K., Swierczynski M.
Polskie Archiwum Hydrobiologii
|
2000
|
tom 47
|
nr 2
11

Copulatory behaviour of the bank vole Myodes glareolus: matings with one and two males do not make a difference

84%
Borkowska A.
Acta Theriologica
|
2010
|
tom 55
|
nr 4
In promiscuous species in which females mate with more than one male during oestrus, males may increase their sperm expenditure or change their copulatory behaviour in response to the risk of sperm competition. I used an experimental approach to investigate the pattern of copulatory behaviour of the bank voleMyodes glareolus Schreber, 1780 depending on whether the female mated with one or two males. The work showed that the copulatory period of the bank vole lasted about 80 minutes and consisted of 4–5 ejaculatory series, with multiple intromissions preceding ejaculation. There were no significant changes in number of intromissions across the first four ejaculatory series, but I did find a relationship between number of intromissions and first ejaculation latency; also, ejaculation latencies grew shorter as the ejaculatory series proceeded. Litter size did not differ significantly between females that mated with one male and those mating with two, nor did the reproductive success of males that mated with the same female. Mating with an oestrus female appears to be advantageous for bank vole males even if they mate as the second one, and the risk of sperm competition did not trigger changes in male copulatory behaviour. The similar durations of the copulatory period and patterns of change of ejaculation latencies during copulations with one and two males point to the role of the female in temporal copulatory behaviour of the bank vole.
12

The variability of common reed Phragmites australis (Cav.) Trin. ex Steudel populations growing in urban conditions

84%
Krzakowa M., Jakubiak H., Kolodziejczak M.
Ecological Questions
|
2008
|
tom 10
Differences between 13 common reed (Phragmites australis) populations, growing in urban conditions within the town of Poznań (western Poland), are described by 8 morphological traits of panicles' variability and the frequency of peroxidase (dimeric and monomeric) allozymes. Values of morphological characters were processed statistically using agglomerative clustering by the closest neighbours (UPGMA) method based on Euclidean distances. Proteins were separated in the starch gel electrophoretic procedure, showing cathodic migration. Populations are polymorphic and have a certain level of heterozygosity. The level of populations' diversity (DST = 0.097) is lower than the intra-population variability (GST = 0.187). The gene flow between populations is rather low (Nm = 1.090).
13

Allozyme variability and differentiation in Serbian roe deer populations Capreolus capreolus

84%
Milosevic-Zlatanovic S., Crnobrnja-Isailovic J., Stamenkovic S.
Acta Theriologica
|
2005
|
tom 50
|
nr 4
429-444
The present study investigates the genetic structure of 12 roe deerCapreolus capreolus Linnaeus, 1758 population samples from Serbia, by screening a total of 334 individuals. We examined whether genetic differentiation exists in local populations in Serbia, and addressed the question whether management policies may affect genetic structure. The populations were analysed by multilocus protein electrophoresis, with 33 protein loci examined. Screening of 20 enzymes and one group of general proteins revealed polymorphism at the following 12 loci: Sdh, Mdh-1, Me-1, Idh-2, 6-Pgd-1,αGpd, Ak, Pgm-1, Pgm-2, Ca, Mpi andGpi. Among samples, the proportion of polymorphic loci varied between 3–15.2% (mean 11.9%), while the average gene diversity was in the range of 1.1–4.2%. The overall genetic differentiation was low (θ = 0.03). The comparison of two regional population groups (northern-southern, separated by the Danube River) showed an absence of genetic differentiation between regions. Gene flow was estimated at 8.96 migrants per generation, and was higher in the lowland than in the highland group. Three loci (Ca, 6-Pgd andGpd-1) showed clinal variation along a geographical gradient. Additional five alleles of four loci (Ak, Pgm-1, Gpi, 6-Pgd) showed significant spatial autocorrelation. Genetic distances were small (D = 0–0.004). Northern and southern populations clustered separately. For at least three populations game management practices provide evidence for outlying genetic parameters. The observed heterogeneity in the inbreeding level was deemed more under the influence of non-random mating strengthened by game management, than by overall selective pressure.
14

Molecular confirmation of introgressive hybridization between whitefish [Coregonus lavaretus] and peled [C.peled] - a case study

84%
Brzuzan P., Luczynski M.
Polskie Archiwum Hydrobiologii
|
1999
|
tom 46
|
nr 3-4
15

Patterns of genetic diversity in Mediterranean supralittoral amphipods [Crustacea, Amphipoda]

84%
De Matthaeis E., Ketmaier V., Davolos D., Schembri P. J.
Polskie Archiwum Hydrobiologii
|
2000
|
tom 47
|
nr 3-4
16

Comparative morphometrical and biochemical-genetic investigations in wild and ranch mink [Mustela vison: Carnivora: Mammalia]

84%
Kruska D., Schreiber A.
Acta Theriologica
|
1999
|
tom 44
|
nr 4
Morphometrical and biochemical-genetic comparisons were performed between wild IMustela vison energúmenos Bangs, 1896) and ranch mink (Dark Standard strain) to investigate intraspecific differences and to characterize effects of the domestication in this species. All animals were kept under similar conditions in larger open air enclosures prior to dissection to keep modificatory influences on the measures low and comparable. In the morphometrical part of this study weights of the total body, brain, eyes, thoracal viscera, heart, abdominal viscera, liver, spleen, kidneys, adrenals, and pancreas of 82 (39 males, 43 females) wild and 97 (50 males, 47 females) ranch mink were compared using the allometrical method with the net carcas weight as the reference parameter. Only three organs were significantly smaller in size in the ranch mink group: brain, heart, and spleen. Size decreases may result from reductions of central nervous and circulatory functions in the domesticated organism. They were compared with results in other species and evaluated as a genetically linked intraspecific adaptation to the special ecological demands of domestication. Twenty five proteins encoded by products of 44 genetic loci were compared electrophoretically between 7 wild and 7 ranch mink. Except for one esterase isozyme locus all genes examined were monomorphic. The protein heterozygosity was rather low in both groups. These results were discussed in connection with certain bottleneck situations, with investigations in other species, and with the short domestication time of ranch mink.
17
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Genetic differences between two Polish populations of Pinus uliginosa, compared to P. sylvestris and P. mugo

84%
Lewandowski A., Samocko J., Boratynska K., Boratynski A.
Dendrobiology
|
2002
|
tom 48
Genetic differences between two populations of P. uliginosa from Batorów and Węgliniec were assessed on the basis of 15 allozyme loci. The level of genetic differentiation between them was also compared with genetic differences among the three closely related pine taxa: P. uliginosa, P. sylvestris and P. mugo. A high level of genetic variation was found in both populations of P. uliginosa. The average (Na) and effective (Ne) numbers of alleles per locus amounted respectively to 2.47 and 1.50 in Węgliniec and to 2.67 and 1.52 in Batorów and the percentage of polymorphic loci was 80% and 87%, respectively. Close relationship between the three studied species were confirmed. The genetic differences between the two populations of P. uliginosa were substantial, as the Nei's genetic distance between the two populations (D = 0.040) was larger than between populations of P. sylvestris and between populations of P. mugo. The relatively high level of genetic differentiation between P. uliginosa populations may result from their isolation, small size and possibly different origin of these populations.
18
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Genetic variation of cowslip [Primula veris L.] populations [West Poland]

84%
Morozowska M., Krzakowa M.
Acta Societatis Botanicorum Poloniae
|
2003
|
tom 72
|
nr 4
Genetic variation of twelve Polish populations of Primula veris L. from western Poland was investigated in respect of six enzyme systems: 6-phosphogluconate dehydrogenase (6PGD), diaphorase (DIA), menadione reductase (MNR), formate dehydrogenase (FDH), isocitrate dehydrogenase (IDH) and glutamate oxaloacetate transaminase (GOT). Only two of them (6PGD and DIA) were polymorphic and all populations were compared according to four loci and eight alleles. For 6PGD only one out of the two detected loci (locus 6PGD-2) was polymorphic and consisted of three alleles a, b and c. For DIA each of two detected loci had two alleles. For 6PGD-2 one population was monomorphic and four populations were monomorphic for DIA-1 and DIA-2. The rest of the populations were polymorphic with low frequency of heterozygotes. The low heterozygosity level, found in the examined populations, was confirmed by high values of the fixation index (F). The level of genetic differentiation among GST populations specified for each polymorphic loci, was equal to 0.045 for 6PGD-2 and had the value of 0.078 for DIA-2 and 0.186 for DIA-1. Nm value for polymorphic loci was 1.10 for DIA-1 and 2.94 for DIA-2, and for 6PGD-2 was 5.33, what indicates some gene flow between the examined populations. The dendrogram constructed on the basis of genotype frequencies showed that the populations were divided into two groups, however the most southern population No. 2 was clearly similar to the northern population No. 8.
19

Notes on genetic differentiation within the endemic Proasellus species group from Lake Ohrid [Balkan Peninsula]

84%
Kilikowska A., Wysocka A., Sell J., Zietara M., Sywula T.
Biological Letters
|
2006
|
tom 43
|
nr 1
20

Morphometric, biochemical and molecular traits in Caucasian wood mice [Apodemus-Sylvaemus], with remarks on species divergence

84%
Hille A., Tarkhnishvili D., Meinig H., Hutterer R.
Acta Theriologica
|
2002
|
tom 47
|
nr 4
We analysed Caucasian wood mice from Georgia (n = 60) and supplementary reference material of the Apodemus/Sylvaemus species group to evaluate the reliability of taxon identification. Traditional "expert knowledge" plus three different methodological approaches were employed and combined to perceive their discriminatory power for a reliable taxon assignment. Graphs of principal component scores derived from the analysis of 14 skull metrics displayed taxon membership of individuals. Individual multi­-locus (L = 18) electrophoretic profiles were used to re-assess specimens to a specific genepool by an assignment test based on allele frequencies indicative of populational taxon samples of the respective sampling locations. Genotyped individuals were re-allocated to those taxa, for which they yielded the highest probability score. Genetic distances among the taxa were computed and clustered in a neighbour-joining tree. PCR-fragments of 1074bp amplified from the mitochondrial cytochrome b gene were cut with 2 six- and 4 four-cutter restriction enzymes, and resulting RFLP patterns were analysed phenetically to classify the specimens according to their molecular similarity. Partial cytochrome b sequences were used to construct a phylogenetic tree by computing neighbour-joining clusters from a matrix of percent nucleotide differences. The power of the combined classification approaches and their congruence is discussed. It is concluded that the joint application of traditional, morphometric and biochemical or genetic tech­niques for taxon allocation of specimens of wood mice encountered problems in species delimitation. The mtDNA topology obtained was not congruent with protein polymor­phism that indicated differential historical and/or recent introgression and incomplete lineage sorting in substructured populations. Cytochrome b sequence DNA data analysed were not as adequate as expected to resolve phylogenetic relationships among Caucasian and European members of the Apodemus-Sylvaemus complex. Altogether, morphometric, biochemical and sequence data sets did not support the hypothesis of the evolutionary independence of European and Caucasian lineages of wood mice. Nonetheless, extended combined morphological and genetic analyses are considered necessary prerequisites to an in-depth study of the evolutionary lineages of the Apodemus/Sylvaemus group. More sequence data of a variety of genes (and plenty of nuclear markers) are needed to resolve the various levels of differentiation of the extant lineages.
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