Wyniki wyszukiwania - AGRO

1

The influence of culture conditions on adhesion of Listeria monocytogenes to hexadecane

100%

Dabrowski W., Iwanski R., Czeszejko K., Medrala D.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2001

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tom 04

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nr 2

Results of various culture conditions on adhesion of Listeria monocytogenes strains to hexadecane are presented. Listeria strains cultured in optimal conditions adhered to this hydrophobic carbohydrate to a small extent. It reveals that their cell walls have hydrophilic properties. Application of low-nutrient medium, incubation at low temperature, acidification of medium to pH 5 and addition of 8% of NaCl caused transformation of strains into hydrophobic. Growing in vacuum conditions produced the opposite effect all strains were extremely hydrophilic. Changes of strain properties from hydrophilic into hydrophobic affected analysed strains to a different degree. One of tested strains continued to be hydrophilic regardless of culture conditions. The other a standard strain isolated from patient became hydrophobic most frequently.

2

The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

75%

Basson M. D., Zeng B., Wang S.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

3

Effect of alpha3beta1 and alphavbeta3 integrin glycosylation on interaction of melanoma cells with vitronectin

75%

Janik M. E., Przybylo M., Pochec E., Pokrywka M., Litynska A.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

55-61

The metastatic transformation of melanocytes is associated with altered expression of adhesion molecules, including αvβ3 and α3β1 integrins. Integrin αvβ3 is a primary vitronectin (VN) receptor, while both integrin types take part in adhesion to VN when they are in complex with uPAR. Although their role in melanoma cell interaction with VN is of great interest, the influence of N-oligosaccharides attached to these glycoproteins is still unappreciated. The present study assesses the role of αvβ3 and α3β1 integrins and the influence of their glycosylation status on WM9 and WM239 metastatic melanoma cell interactions with VN. Cell adhesion to and migration on VN were selected as the studied cell behaviour parameters. Functionblocking antibodies and swainsonine (SW) treatment were used in these tests. Both cell lines interacted with VN in an integrin-mediated but cell-line-specific manner. In WM9 cells, migration was not completely inhibited by antibodies against α3β1 or αvβ3 integrins, suggesting the participation of other VN receptors. In both cell lines in coprecipitation test the formation of an integrins/uPAR complex was shown. In the presence of SW formation of the complex did not occur, suggesting the participation of glycosylation in this proccess. Additionally, the adhesion properties of WM9 cells were changed after SW treatment. Our results suggest that in these two metastatic cell lines integrin-linked N-oligosaccharides influence the VN adhesion receptor activity and function.

4

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Antibacterial modification of polymer veneers

75%

Novak I., Chodak I., Sedliacik J., Popelka A., Prachar J., Matyasovsky J., Jurkovic P.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2015

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tom 92

5

Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

75%

Markowicz C., Schmidt M. T.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2015

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tom 14

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nr 3

Background. The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus. Material and methods. Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR. Results. The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep- PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract. Conclusions. Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.

6

Impact of DNA microarray data transformation on gene expression analysis - comparison of two normalization methods

75%

Schmidt M. T., Handschuh L., Zyprych J., Szabelska A., Olejnik-Schmidt A. K., Siatkowski I., Figlerowicz M.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are; available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results.In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.

7

Gdy klej pracuje

75%

W K.

Biuletyn Informacyjny Ośrodka Badawczo-Rozwojowego Przemysłu Płyt Drewnopochodnych w Czarnej Wodzie

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1999

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tom 40

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nr 3

189-191

8

In vitro culture of human epithelial cells on a modified xenogenic collagen support

75%

Lesiak-Cyganowska E., Sladowski D., Jankowska E., Komender J.

Archivum Immunologiae et Therapiae Experimentalis

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2001

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tom 49

|

nr 3

253-259

9

Lactobacillus spp. belonging to the Casei group display a variety of adhesins

75%

Konieczna C., Olejnik-Schmidt A., Schmidt M. T.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2018

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tom 17

|

nr 1

10

Adhesive movement characteristics of the snail Achatina fulica

75%

Kaczorowski P., Piskorska E., Olszewska-Slonina D., Grzesk G.

Folia Biologica

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2017

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tom 65

|

nr 2

11

Parameters of interface between shrinkable and expansive concrete resulting from their adhesion

75%

Halicka A.

Electronic Journal of Polish Agricultural Universities. Series Civil Engineering

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2007

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tom 10

|

nr 4

12

Resistance of bacterial biofilms formed on stainless steel surface to disinfecting agent

75%

Krolasik J., Zakowska Z., Krepska M., Klimek L.

Polish Journal of Microbiology

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2010

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tom 59

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nr 4

The natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. The aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. A universally applied disinfecting agent was used in the tests. Bacterial strains: Listeria innocua, Pseuciomonas putida. Micrococcus luteus, Staphylococcus hominis strains, were isolated from food contact surfaces, after a cleaning and disinfection process. The disinfecting agent was a commercially available acid specimen based on hydrogen peroxide and peroxyacetic acid, the substance that was designed for food industry usage. Model tests were carried out on biofilm formed on stainless steel (type 304, no 4 finish). Biofilms were recorded by electron scanning microscope. The disinfecting agent in usable concentration, 0.5% and during 10 minutes was ineffective for biofilms. The reduction of cells in biofilms was only 1-2 logarithmic cycles. The use of the agent in higher concentration - 1% for 30 minutes caused reduction of cell number by around 5 logarithmic cycles only in the case of one microorganism, M. luteus. For other types: L. innocua, P. putida, S. hominis, the requirements placed on disinfecting agents were not fulfilled. The results of experiments proved that bacterial biofilms are resistant to the disinfectant applied in its operational parameters. Disinfecting effectiveness was achieved after twofold increase of the agent's concentration.

13

Akt1 binds focal adhesion kinase via the Akt1 kinase domain independently of the pleckstrin homology domain

75%

Basson M. D., Zeng B., Wang S.

Journal of Physiology and Pharmacology

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2015

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tom 66

|

nr 5

14

Influence of wood surface processing on roughness and wetting

75%

Slabejova G., Smidriakova M.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2014

|

tom 87

15

Two-dimensional non-linear, finite element analysis of soil cutting

75%

Kerenyi G., Jori I. J.

Bibliotheca Fragmenta Agronomica

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1997

|

tom 2B

16

The L1 family of neural cell adhesion molecules-linking cell adhesion to membrane skeleton organization

75%

Hortsch M., Dubreuil R. R.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 2

17

The attachment of Helicobacter pylori strains to human epithelial cells

75%

Chmiela M., Rudnicka W., Czkwianianc E., Planeta-Malecka I., Wadstrom T.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

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nr 1

18

The role of tyrosine kinase in the adhesion and fusion of myoblasts

75%

Wrobel E., Moraczewski J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

19

Adhesion and metastases

75%

Janik P., Malecki M.

Cellular and Molecular Biology Letters

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2002

|

tom 07

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nr Suppl.

20

Plasma chlorination of elastomer surfaces

75%

Tyczkowski J., Krawczyk I., Wozniak B.

Acta Agrophysica

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2002

|

tom 80

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