Wyniki wyszukiwania

1

Cultivation of circulating tumor cells in esophageal cancer

100%

Bobek W., Matkowski R., Gurlich R., Grabowski K., Szelachowska J., Lischke R., Schutzner J., Harustiak T., Pazdro A., Rzechonek A., Kolostova K.

Folia Histochemica et Cytobiologica

|

2014

|

tom 52

|

nr 3

2

A correlation between the expression of nucleolar organizer regions [AgNORs] and some clinico-pathological features of sigmorectal adenocarcinomas

100%

Famulski W., Zalewski B., Sulkowski S., Miller-Famulska D., Sulkowska M.

Folia Histochemica et Cytobiologica

|

1999

|

tom 37

|

nr 2

3

Ras oncogene product p21 expression in adenocarcinoma of human endometrium

100%

Miturski R., Semczuk A., Jakowicki J. A.

Cellular and Molecular Biology Letters

|

1996

|

tom 01

|

nr 2

ras-encoded p21 protein expression was investigated immunohistochemically in 10 specimens of human endometrial adenocarcinoma. The control group consisted of 5 proliferative endometrium slides. The analysis was performed by using the biotin-streptavidin-peroxidase AEC detection system. An immunohistochemical histologic H- score according to Nyholm et al. [1] was calculated in a semiquantitative fashion incorporating intensity and distribution of staining. Adenocarcinoma glandular cells stained positively in 6 out of 10 specimens (n=6; H-score 0.71; range 0.3-1.15), whereas adenocarcinoma stromal cells exhibited a reddish-brown color in all specimens analyzed (n=10; H-score 0.83; range 0.3-1.5). Only one out of 5 proliferative endometrium slides stained positively for ras p21 (H-score 0.3). Our observations suggest that in human endometrial adenocarcinomas not only glandular but also stromal cells reacted strongly with monoclonal antibody NCC-RAS-001 raised against ras p21 protein. Enhanced ras p21 expression adenocarcinoma stromal cells in comparison with adenocarcinoma glandular cells may suggest that stromal cells are involved strongly in the neoplasm formation.

4

Neuroendocrine component of different types of the pulmonary carcinoma

100%

Biczysko W., Mlynarczyk W., Blotna-Filipiak M., Przybylowski P.

Folia Morphologica

|

1994

|

tom 53

|

nr 3

5

Patterns of immunohistochemical staining for p53 expression in hyperplastic endometrium and adenocarcinoma

100%

Terlikowski S., Lenczewski A., Sulkowska M., Famulski W., Sulkowski S., Kulikowski M.

Folia Histochemica et Cytobiologica. Supplement

|

2001

|

tom 39

|

nr 1

6

Toll-like receptors and the tendency of normal mucous membrane to transform to polyp or colorectal cancer

86%

Niedzielska I., Niedzielski Z., Tkacz M., Orawczyk T., Ziaja K., Starzewski J., Mazurek U., Markowski J.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 1

65-71

The aim of this study was to identify differences in Toll-like receptor (TLR) expression patterns in normal and diseased tissues of patients with polyps and colorectal cancer. Eight patients were included in the study group (aged 38 to 72 years). Sixteen HG-U133A oligonucleotide microarrays were analysed including four of colonic polyps, four of adenocarcinoma with different degree of histological differentiation (2 poorly and 2 highly differentiated), and eight of macroscopically normal tissue. The levels of selected TLR mRNA transcripts were analysed. An analysis of all per cent variability values with regard to malignancy stage increasing from polyp to stages I to III adenocarcinoma, and normal colon mucosa shows a statistically significant relationship for TLR2 (increasing) and TLR3 (decreasing). In polyps, copy numbers of TLR3, TLR4 and TLR5 mRNA were the highest and TLR7 mRNA the lowest. In normal colon mucosa of polyposis patients the highest mRNA copy numbers were observed for TLR3, and the lowest for TLR7. TLR3 may serve as a marker of colon tissue metaplasia and may indicate the tendency of normal tissue to form polyps transforming to colorectal cancer.

7

Beta-actin in human colon adenocarcinoma cell lines with different metastatic potential

86%

Nowak D., Skwarek-Maruszewska A., Zemanek-Zboch M., Malicka-Blaszkiewicz M.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 2

Human colon adenocarcinoma LS180 parental cell line and selected variants, characterized by different metastatic capacity were used to examine, whether a correlation exists between β-actin expression, its subcellular distribution and metastatic potential of these cells. Cytosolic fraction (supernatant 105 000 × g), isolated from the tumor cells was used as a source for actin quantification. The higher level of β-actin was observed in the cytosol of three selected sublines to compare with LS180 parental line. Statistically significant increase of β-actin level in highly motile EB3 cells variant should be underlined to compare with the other sublines. Distinct differences in the phenotype of adenocarcinoma cell variants were found, such as the changes in cells shape, cells spreading and ability to attach to the surface of culture dish. Actin cytoskeleton was visualized with fluorescence microscopy application and microfilaments rhodamine-conjugated phalloidin staining. β-actin subcellular localization was done by immunofluorescence staining with monoclonal anti-β actin antibodies. In the elongated cells (LS180, 3LNLN), this isoactin is dispersed in the whole cell body and concentrates in pseudopods and at the leading edges, when in the rounded variant (EB3) β-actin dominates mainly in cortical ring under cellular membrane and it is also seen in the subtle protrusions. Summary of our former (Nowak et al., 2002, Acta Biochim. Polon., 49: 823) and current data lead to the conclusion that there is a distinct correlation between metastatic capacity of examined human colon adenocarcinoma cells, the state of actin polymerization, actin cytoskeleton organization and β-actin expression.

8

Bcl-2 expression in relation to progesterone and estrogen receptors status in endometrial adenocarcinoma in women

86%

Semczuk A., Miturski R., Gogacz M., Tomaszewski J., Jakowicki J. A.

Cellular and Molecular Biology Letters

|

1997

|

tom 02

|

nr 2

9

Inhibitory effect of acetylsalicylic acid on metalloproteinases activity in human lung adenocarcinoma at different stages of differentiation

86%

Karna E., Palka J.

Folia Histochemica et Cytobiologica. Supplement

|

2001

|

tom 39

|

nr 1

10

Involvement of CD8plus cells in protective mechanisms in canice mammary adenocarcinomas [short communication]

86%

Nowak M., Madej J. P., Rossowska J., Madej J. A.

Bulletin of the Veterinary Institute in Puławy

|

2007

|

tom 51

|

nr 3

The study aimed at demonstration of involvement of CD8+ cells in protective mechanisms in canine mammary gland carcinomas. Material for the studies involved metastases to lungs sampled at autopsy from crossbreed bitches aged 11 to 13 years. The tumours were verified histopathologically as adenocarcinomas. The presence of CD8+ cells infiltrating the examined tumour tissues was estimated in cryo-sections by immunofluorescence methods. Parental tumours free of metastases were accompanied by slight infiltration of CD8+ cells. In the case of metastasing tumours, and especially in their pulmonary metastases, the increased number of CD8+ cells was observed. Taken together, metastasing potential of canine mammary adenocarcinomas was positively correlated with the number of CD8+ cells in the tumour stroma.

11

Treatment of thyroid adenocarcinoma in dog by surgigal resection alone-case report

86%

Adamiak Z., Brzeski W., Nowicki M., Rotkiewicz T., Babinska I.

Acta Scientiarum Polonorum. Medicina Veterinaria

|

2002

|

tom 01

|

nr 1

12

5-aminolevulinic acid photodynamic therapy of transplantable colon adenocarcinoma in BALB-c mice

86%

Ziolkowski P., Symonowicz K., Osiecka B. J., Rabczynski J.

Archivum Immunologiae et Therapiae Experimentalis

|

1998

|

tom 46

|

nr 5

13

Proliferative activity in endometrial hyperplasia and adenocarcinoma

86%

Terlikowski S., Lenczewski A., Famulski W., Sulkowska M., Kulikowski M.

Folia Histochemica et Cytobiologica

|

2001

|

tom 39

|

nr 2

14

Nonhistone chromosomal proteins [NHCP] in adenocarcinoma of human endometrium

86%

Miturski R.

Acta Biochimica Polonica

|

1995

|

tom 42

|

nr 2

Concentration and electrophoretic pattern of nonhistone chromosomal proteins (NHCP) in cancer and normal endometrium were examined. In normal tissues the NHCP/DNA ratio ranged from 0.6 to 0.8 whereas in malignant tissues from 1.2 to 1.7. Mean nuclear concentration of NHCP was 14.16 pg/cell nucleus of normal endometrium and 33.35 pg/cell nucleus of cancer tissue. The two tissues differed in the electrophoretic pattern. Heavy fractions (>60 kDa — 36%) predominated in normal tissue whereas light fractions (< 30 kDa — 48%) in malignant tissue samples. The percentage of intermediate fractions (30-60 kDa) was similar. Neoplastic endometrium shows, irrespective of the patient's age, a high NHCP content. It seems that NHCP, especially the fraction extracted with 0.35 M NaCl, because of its tissue and cell specificity are involved in neoplasm formation.

15

Silencing of the type 1 insulin-like growth factor receptor increases the sensitivity to apoptosis and inhibits invasion in human lung adenocarcinoma A549 cells

72%

Ma Z., Dong A., Kong M., Qian J.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 4

556-572

The type 1 insulin-like growth factor receptor (IGF-1R), which is over-expressed or activated in many human cancers, including lung cancer, mediates cancer cell proliferation and metastasis. Several studies indicate that blocking IGF-1R expression can inhibit tumor cell proliferation and metastasis. In this study, inhibition of the endogenous IGF-1R by recombinant adenoviruses encoding short hairpin RNAs against IGF-1R was found to significantly suppress IGF-1R expression, arrest the cell cycle, enhance the apoptotic response, and inhibit proliferation, adhesion, invasion and migration in A549 cells. Moreover, silencing IGF-1R decreases the expression of invasive-related genes including matrix metalloproteinase-2 (MMP-2), MMP-9, and urokinase-plasminogen activator (u-PA), and the phosphorylation of Akt and ERK1/2. These results suggest that the silencing of IGF-1R has the potential to be an effective cancer gene therapy strategy for human lung cancer.

16

Prognostic significance of DAPK and RASSF1A promoter hypermethylation in non-small cell lung cancer [NSCLC]

72%

Niklinska W., Naumnik W., Sulewska A., Kozlowski M., Pankiewicz W., Milewski R.

Folia Histochemica et Cytobiologica

|

2009

|

tom 47

|

nr 2

275-280

17

PCR-based methods for detection of JSRV in experimentally and naturally infected sheep

72%

Kycko A., Reichert M.

Bulletin of the Veterinary Institute in Puławy

|

2010

|

tom 54

|

nr 4

Application of real-time PCR using Taqman probe was tested for jaagsiekte sheep retrovirus (JSRV) detection. Sensitivity of real-time PCR and hemi-nested PCR methods was compared using plasmid DNA. The methods, along with RT PCR and real-time RT PCR, were tested for the possibility of JSRV genome (LTR region) detection in biological material from experimentally and naturally infected sheep. The experimental group of eight animals was used, including five lambs infected with JSRV by intratracheal inoculation at the age of 2 weeks. The samples collected from the animals ante-mortem included blood and respiratory tract fluid. Lung tissue, mediastinal lymph nodes, spleen, and liver were collected post-mortem. The field studies included blood samples collected from sheep from Polish flocks and lung samples obtained from slaughterhouse. In addition, DNA samples isolated from blood of sheep from the abroad located flocks with history of ovine pulmonary adenomatosis (OPA) were also included. Lung samples were examined histologically for the presence of pulmonary adenocarcinoma. The sensitivity of PCR, hemi-nested PCR, and real-time PCR using Taqman probe was evaluated as 10³, 10², and 10² viral copies, respectively. Both viral RNA and DNA were detected in the lung fluid taken from JSRV infected sheep showing clinical sings of OPA and in all neoplastic tissues. Proviral DNA was found in mediastinal lymph node of one experimental sheep. Five of the 66 DNA samples from the abroad located farms were positive for the presence of JSRV LTR. All blood and lung samples collected from Polish sheep were negative for the presence of JSRV LTR. The characteristic adenocarcinoma lesions were found in all lung sections of experimentally infected sheep. Implementation of the real-time PCR method is a good alternative to traditional PCR and hnPCR in JSRV detection and, apart from histopathological and immunohistochemical examinations, may be used as a confirmatory test in clinically suspected cases, or as a screening method in control or eradication scheme.

18

Immunoexpression of P16INK4a, Rb and TP53 proteins in bronchiolar columnar cell dysplasia [BCCD] in lungs resected due to primary non-small cell lung cancer

72%

Pankiewicz W., Sulewska A., Niklinska W., Naumnik W., Laudanski J., Niklinski J., Chyczewski L.

Folia Histochemica et Cytobiologica

|

2008

|

tom 46

|

nr 1

89-96

19

Inhibition of Barrett's adenocarcinoma cell growth by simvastatin: involvement of COX-2 and apoptosis-related proteins

72%

Konturek P. C., Burnat G., Hahn E. G.

Journal of Physiology and Pharmacology. Supplement

|

2007

|

tom 58

|

nr 3

141-148

20

Comparison of cellular and tissue transcriptional profiles in canine mammary tumor

72%

Pawlowski K. M., Krol M., Majewska A., Badowska-Kozakiewicz A., Mol J. A., Malicka E., Motyl T.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 1

85-94

Tumor-derived cell lines are widely used as in vitro cancer models. Cell lines historically served as the primary experimental model systems for exploration of tumor cell biology and pharmacology. However, their ability to accurately reflect the phenotype and genotype of the parental histology remains questionable, given the prevalence of documented cell line-specific cytogenetic changes. Sometimes cell line studies are interpreted in the context of artifacts introduced by selection and establishment of cell lines in vitro. This complication has led to difficulties in the extrapolation of biology observed in cell lines to tumor biology in vivo. The aim of our study was to compare gene expression profiles in canine mammary tumor tissue and cell cultures derived from those tumors using cDNA microarrays. Tumors of two different origins were used; chondrosarcoma and adenocarcinoma and their primary cell cultures. It has been found that cell culture gene expression profiles closely resembled those of their corresponding in vivo tumor. In adenocarcinoma and chondrosarcoma only 6.0% and 2.7% of genes respectively, have shown significant difference in expression. In the most cases the difference concerned up-regulation of gene expression in cell lines, particularly genes involved in: protein metabolism and modification, signal transduction and nucleotide, nucleoside and nucleic acid metabolism. These experiments revealed that transcriptome of our primary cell culture corresponds to transcriptome of its parental tumor tissue and for this reason cell culture represents the reliable in vitro model for oncogenomic and pharmacogenomic studies.

Ograniczanie wyników

Cultivation of circulating tumor cells in esophageal cancer

A correlation between the expression of nucleolar organizer regions [AgNORs] and some clinico-pathological features of sigmorectal adenocarcinomas

Ras oncogene product p21 expression in adenocarcinoma of human endometrium

Neuroendocrine component of different types of the pulmonary carcinoma

Patterns of immunohistochemical staining for p53 expression in hyperplastic endometrium and adenocarcinoma

Toll-like receptors and the tendency of normal mucous membrane to transform to polyp or colorectal cancer

Beta-actin in human colon adenocarcinoma cell lines with different metastatic potential

Bcl-2 expression in relation to progesterone and estrogen receptors status in endometrial adenocarcinoma in women

Inhibitory effect of acetylsalicylic acid on metalloproteinases activity in human lung adenocarcinoma at different stages of differentiation

Involvement of CD8plus cells in protective mechanisms in canice mammary adenocarcinomas [short communication]

Treatment of thyroid adenocarcinoma in dog by surgigal resection alone-case report

5-aminolevulinic acid photodynamic therapy of transplantable colon adenocarcinoma in BALB-c mice

Proliferative activity in endometrial hyperplasia and adenocarcinoma

Nonhistone chromosomal proteins [NHCP] in adenocarcinoma of human endometrium