Wyniki wyszukiwania

1

Setaria cervi collagenase: IgG cleavage and inhibition by Wuchereria bancrofti infected sera

100%

Srivastava Y., Gupta S., Pokharel D. R., Rathaur S.

Acta Parasitologica

|

2004

|

tom 49

|

nr 3

Significant protease activity has been detected in somatic extract of adults and microfilarial stage of Setaria cervi, using general protease substrates and collagen. The pH optima studies of the somatic extract of adult females showed two peaks at 7.0 and 5.0 for collagenase activity. Both forms were purified using sequential DEAE-sepharose and Sephadex G-100 column chromatography. The purified enzymes had the molecular masses of 175 and 20 kDa and pH optima at 7.0 and 5.0, respectively. The 175 kDa collagenase was more sensitive to metal chelators and serine protease inhibitors. However, 20 kDa collagenase was sensitive to cysteine protease inhibitors. The IgG antibodies from W. bancrofti infected human sera inhibited both enzymes. Further the purified collagenases were used to digest total human IgG at their respective pH and for different lengths of time. The 175 kDa protein was capable of cleaving human IgG. The digestion appeared to be restricted to a single cleavage point of H-chain within the hinge region of IgG molecule and produced fragments of similar molecular mass (27 kDa) indicating cleavage to Fab and Fc fragments. The degree of digestion was found to be proportional to the incubation time at 37°C. No further digestion of either fragments were observed. The L-chains were apparently resistant to collagenase digestion in all cases. Thus, the results suggest that S. cervi collagenase might be involved in the defense mechanisms of the parasite against the immune response of the host.

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Lymphatic filariasis among the Ezza people of Ebonyi State, eastern Nigeria

84%

Anosike J. C., Nwoke B. E., Ajayi E. G., Onwuliri C. O., Okoro O. U., Oku E. E., Asor J. E., Amajuoyi O. U., Ikpeama C. A., Ogbusu F. I., Meribe C. O.

Annals of Agricultural and Environmental Medicine

|

2005

|

tom 12

|

nr 2

181-186

A total of 1,243 Ezza people living in 10 communities of Ebonyi State, eastern Nigeria were examined between July 2002-January 2003 for lymphatic filariasis. This is the first time a filariasis survey due to Wuchereria bancrofti has been carried out in this state. Of the 1,243 persons examined, 210 (16.9%) had W. bancrofti microfilariae. Infection varied significantly among communities and ages (p<0.05) but not sex-related (p>0.05). The Ezza people are predominantly farmers and professional hired labourers. There was a close association between microfilaria rate and microfilaria -density in various age groups (r = 0.812; p<0.01). Microfilaria density is an important measure in the epidemiology, treatment and control of human filarisis in this endemic foci. Clinical signs and symptoms of the disease include elephantiasis, hydrocoele, dermatitis and periodic fever. Clinical symptoms without microfilaraemia and microfilaraemia without clinical symptoms were also observed. Of 1,603 mosquitoes dissected, Anopheles gambiae, An. funestus and Culex quinquefasciatus showed infectivity rates of 6.3%, 5.1% and 6.0% respectively. The affected persons and other key informants are unaware of the cause of the disease and attributed it to witchcraft, violation of taboo, bad water and food. Intervention strategies to be integrated into the on-going Community-Directed Treatment with Ivermectin (CDTI) project are discussed.

3

In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis

84%

Saeed M., Adnan M., Khan S., Al-Shammari E., Mustafa H.

Acta Parasitologica

|

2016

|

tom 61

|

nr 1

4

Vectors of lymphatic filariasis of human

67%

Okulewicz A., Fronska-Popiel A.

Wiadomości Parazytologiczne

|

1999

|

tom 45

|

nr 4

5

Helmintozy malp - antropozoonozy ?

51%

Zuchowska E.

Magazyn Weterynaryjny

|

1995

|

tom 04

|

nr 2

107-108

6

Filariozy zwierząt i ludzi

51%

Soltysiak Z., Frydrychowski P., Noszczyk-Nowak A.

Magazyn Weterynaryjny. Zeszyt Edukacyjny

|

2017

|