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Use of the chemical permeabilization process in yeast cells: production of high-activity whole cell biocatalysts

100%

Trawczynska I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2020

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tom 101

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nr 3

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Fractal morphology of Beta vulgaris L. cell suspension culture permeabilized with Triton X-100

100%

Arenas-Ocampo M., Alamilla-Beltran L., Vanegas-Espinoza P. E., Camacho-Diaz B. H., Campos-Mendiola R., Gutierrez-Lopez G., Jimenez-Aparicio A.

International Agrophysics

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2012

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tom 26

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nr 1

n this work, morphology of Beta vulgaris L. cells permeabilized with 0.7 mM of Triton X-100® was evaluated using digital image processing and concepts of fractal dimension (perimeter- area relations). Important morphometric changes were found when the contact-time with chemical agent was increased.The size of cells decreased, the cells lost the roundness and their shape was more sinuous; this behaviour was a result of a probable shrinkage caused by the excess of exposure with the permeabili- zation agent. Morphology of B. vulgaris cells after permeabili- zation, exhibited a fractal nature since the slope of the ratio of the logarithm of the perimeter vs logarithm of the area was higher than unit. Fractal geometry of the cell morphology was affected as a re- sult of the exposure to Triton X-100®. Those changes can be attri- buted to the loss of turgor and structure of the cell wall.

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Divalent cations and protons inhibit Triton X-100 induced ion channels

100%

Rostovtseva T. K.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

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Effect of Triton X-100 on the activity and solubilization of rat liver mitochondrial phosphatidylserine decarboxylase

84%

Dygas A., Zborowski J.

Acta Biochimica Polonica

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1989

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tom 36

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nr 2

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Water evacuation from pea seeds previously treated with HgCl2 and surfactants (SDS, Triton X-100)

84%

Poliszko S., Plenzler G., Klimek-Poliszko D., Napierala D.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

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2008

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tom 11

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nr 2

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Oxyethylene chain-cation complexation; Nonionic polyoxyethylene detergents attain a positive charge and demonstrate electrostatic head group interactions

84%

Hagerstrand H., Bobacka J., Bobrowska-Hagerstrand M., Kralj-Iglic V., Fosnaric M., Iglic A.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 2

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Damage of erythrocyte membranes induced by digitonin

84%

Bielawski J., Knopik A., Szczuka E.

Biological Bulletin of Poznań

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1996

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tom 33

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Modification of the lipid component modulates nuclear matrix nucleolytic activity

84%

Lubocka J., Szopa J., Kozubek A.

Acta Biochimica Polonica

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1995

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tom 42

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nr 2

It was shown that lipid composition of plant nuclear matrix depends on procedure of its isolation. The matrix isolated with the use of lithium diodosalicylate (LiS) differs in its lipid composition from the preparation isolated with the use of nonionic detergent (Triton X-100). It was also shown that the nucleolytic activity of the matrix is related to its lipid component. Matrix depleted in lipids loses half of its nucleolytic activity which is recovered after supplementation with previously extracted lipids. The extent of recovery of the nucleolytic activity is also dependent on the presence of residual DNA in matrix preparation. The recoveries of nucleolytic activities were higher in matrices not depleted in their DNA content.

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Interaction of the Pisum sativum nuclear matrix proteins with SAR DNA

84%

Rzepecki R., Markiewicz E., Adamiec R., Szopa J.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

We have isolated the nuclear matrices from Pisum sativum cell nuclei using three methods: i. standard procedure involving extraction of cell nuclei with 2 M NaCl and 1% Triton X-100; ii. the same with pretreatment of cell nuclei with 0.5 mM CuS04 (stabilisation step); and iii. method including lithium diiodosalicylate extraction. We compared the polypeptide pattern and residual DNA content of the nuclear matrices isolated. The nuclear matrices displayed a specific endonuclease activity which was due to the presence of a 32 kDa protein. The isolated nuclear matrices bound specifically the scaffold-attached (SAR) DNA derived from human p interferon gene, in the exogenous SAR binding assay. Using the DNA-protein binding blot assay we demonstrated the presence of two nuclear matrix proteins of 66 kDa and 62 kDa which bound specifically SAR DNA.

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Evidence for the involvement of a 66 kDa membrane protein in the synthesis of sterolglucoside in Saccharomyces cerevisiae

67%

Lenart U., Haplova J., Magdolen P., Farkas V., Palamarczyk G.

Acta Biochimica Polonica

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1995

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tom 42

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nr 2

The membrane-bound sterolglucoside synthase from the yeast Saccharomyces cerevisiae has been solubilized by nonionic detergent, Nonidet P-40, Triton X-100, and partially purified by DEAE-cellulose column chromatography and ammonium sulfate fractionation. SDS/PAGE of the purified fraction revealed the presence of two protein bands of molecular mass 66 kDa and 54 kDa. In an attempt to identify further the polypeptide chain of sterolglucoside synthase, the partially purified enzyme was treated with [di-125I]-5-[3-(p-azidosalicylamide)]allyl-UDPglucose, a photoactive analogue of UDPglucose, which is a substrate for this enzyme. Upon photolysis the 12SI-labeled probe was shown to link covalently to the 66kDa protein. The photoinsertion was competed out by the presence of unlabeled UDPglucose thus suggesting that this protein contains substrate binding site for UDPglucose. Since photoinsertion of the probe to protein of 66 kDa correlates with the molecular mass of the protein visualized upon enzyme purification we postulate that the 66 kDa protein is involved in sterolglucoside synthesis in yeast.

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Hemolytic activity of the non-ionic detergents Tween 80 and Triton X-100

51%

Bielawski J., Mrowczynska L., Konarczak M.

Biological Bulletin of Poznań

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1995

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tom 32

Ograniczanie wyników

Use of the chemical permeabilization process in yeast cells: production of high-activity whole cell biocatalysts

Fractal morphology of Beta vulgaris L. cell suspension culture permeabilized with Triton X-100

Divalent cations and protons inhibit Triton X-100 induced ion channels

Effect of Triton X-100 on the activity and solubilization of rat liver mitochondrial phosphatidylserine decarboxylase

Water evacuation from pea seeds previously treated with HgCl2 and surfactants (SDS, Triton X-100)

Oxyethylene chain-cation complexation; Nonionic polyoxyethylene detergents attain a positive charge and demonstrate electrostatic head group interactions

Damage of erythrocyte membranes induced by digitonin

Modification of the lipid component modulates nuclear matrix nucleolytic activity

Interaction of the Pisum sativum nuclear matrix proteins with SAR DNA

Evidence for the involvement of a 66 kDa membrane protein in the synthesis of sterolglucoside in Saccharomyces cerevisiae

Hemolytic activity of the non-ionic detergents Tween 80 and Triton X-100