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The Syringa genus is characterizedby a multiplicity of forms. Its chief asset is the ornamental value of thousands of accessions, species or hybrids. From a phylogenetic point of view the genus is difficult in an explicit classification due to its frequently complex genome. The aim of this study was to determine the possibility for the identification of genotypic diversity and genetic relationships in the nrDNA sequence of some selected Syringa accessions – part of a collection of the Dendrological Garden in Przelewice (Poland). For this purpose, the PCR technique together with a combination of various ‘universal’ primers designed for the nrDNA sequence analysis were employed. Fourteen Syringa accessions: Syringa × chinensis Willd., S. × prestoniae Mc Kelv., S. × prestoniae ‘Telimena’, S. × prestoniae ‘Jaga’, S. × prestoniae ‘Basia’, S. meyeri ‘Palibin’, S. vulgaris ‘Miss Ellen Willmott’, S. vulgaris, S. vulgaris ‘Jules Simon’, S. vulgaris ‘Katherine Havemeyer’, S. vulgaris ‘Krasawica Moskvy’, S. vulgaris ‘Mirabeau’, S. vulgaris ‘Madame Lemoine’ and S. vulgaris ‘Niebo Moskvy’ made up the research material. In the conducted amplifications, genetic profiles were obtained for 14 combinations among the 25 combinations of different pairs of primers used. The nrDNA templates coding the small subunit (SSU), 5.8S subunit andITS1, ITS2 andIGS sequences were amplified. In PCR reactions a total of 33 PCR products were generated, of which 21 (64%) products were polymorphic, 6 (18%) monomorphic and6 (18%) were genotype-specific. For the lilac accessions examined246 amplicons were generated from ~230 to ~1100 bp in length. The analysis of both the dendrogram and the genetic similarity matrix revealedlow diversity between the examinedaccessions. For most they rangedfrom 70 to 80%, andthe greatest diversity (87%) was foundbetween the S. × prestoniae: ‘Basia’ and‘Telimena’ accessions, while the lowest (57%) was observed between S. vulgaris ‘Katherine Havermeyer’ and S. × chinensis.
Phenological observations at the Botanical Garden of the Adam Mickiewicz University in Poznań were conducted on Syringa komarowii, S. meyeri, S. microphylla, S. oblata, S. reticulata, S. tomentella and S. wolfii. These observations showed the longest foliation phase in the case of S. oblata and S. meyeri, while the longest flowering phase was recorded in S. reticulata, S. microphylla and S. wolfii. Additionally, the longest period of anthesis (i.e. the period from the moment when 25% flowers bloomed to the time when 75% were out of bloom) was observed in S. oblata and S. microphylla. All examined shrubs, except for S. oblata, set fruits, which dispersal was extended to the next year (except for S. komarowii). In terms of decorative value suitable for green areas the most valuable species were S. komarowii, S. reticulata, S. meyeri, S. microphylla and S. oblata, with the three latter being the most showy shrubs in terms of autumn foliage. Moreover, S. komarowii was exceptional among the analysed species due to its large, attractive leaf blades. This lilac, together with S. meyeri, was also characterised by the most decorative inflorescences. Thanks to their stronger growth in comparison to the other species, S. komarowii, S. oblata and S. reticulata are best suited for large gardens, whereas S. meyeri and S. microphylla will prove most suitable for small gardens. All the recommended shrubs show good drought resistance.
Inter-simple sequence repeat (ISSR) amplification was used to analyze polymorphism of microsatellite sequences in the lilacs genom and to evaluate genetic diversity among seven lilacs species (Syringa × prestoniae McKelvey., S. reflexa K.C. Schneid., S. villosa Vahl, S. × chinensis Willd., S. meyeri K.C.Schneid., S. vulgaris L.and S. reticulata (Blume) var. amurensis (Rupr.)). The plant material was originated from the collection of Dendrological Garden in Przelewice.A total of 30 primers, containing different simple sequence repeat motifs were tested for amplification.Out of the 30 primers only 13 gave interpretable banding patterns in all lilacs species.A total of 182 ISSR fragments were generated with 13 primers of which 109 (60%) were polymorphic and 57 (31.2%) species-specific. ISSR–PCR with genomic DNAs of the showed lilacs yielded DNA fragmets ranging form 2200 to 123 bp in size.Species-specific ISSR fragments were detected for each lilacs accessions.UPGMA cluster analysis was used to construct a dendrogram and to estimate the genetic distances between lilacs species.The ISSR-based phylogeny was generally consistent with Syringa taxonomy based on morphological and phenological evidence.
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