Wyniki wyszukiwania

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Modifications influencing Widal test reactivity in a novel microplate assay

100%

Almogren A., Shakoor Z., Adam M. H., Gadelrab M. O., Musa H. A.

Polish Journal of Microbiology

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2012

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tom 61

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nr 2

Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. !is study was performed to assess the effect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 + 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among them were 26 patients with suspected typhoid fever and 11 had bacteriologically confirmed diagnosis of Salmonella infection. The modifications included either the use of 0.5% bovine serum albumin (BSA), absorption of sera with sheep red blood cells (SRBC) or heat inactivation of sera. Compared with Widal tube agglutination test, microplate assay with SRBC absorption of the sera from patients with suspected typhoid fever was not only associated with enhancement of detection titers for both H (p < 0.001) and O (p < 0.005) Salmonella agglutinins but also the percentage of reactivity. !e presence of BSA augmented detection titers for Salmonella H agglutinins (p < 0.02) only. Heat inactivation of sera however was found to be associated with reduction in the detectable titers for both H (p < 0.03) and O (p < 0.01) agglutinins. Increased titers of Salmonella agglutinins were also evident in 11 patients with confirmed diagnosis of Salmonella infection. The novel microplate agglutination assay using the SRBC absorption was associated with enhancement in Widal test reactivity and appears to be a useful alternative for the diagnosis of Salmonella infection.

2

Detection of Salmonella spp., Salmonella enteritidis, Salmonella typhi and Salmonella typhimurium in cream cakes by polymerase chain reaction (PCR)

84%

Can H. Y., Elmali M., Karagoz A., Oner S.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 11

The aims of this study were: (i) to determine the incidence of Salmonella spp. by conventional culture methods in cream cakes, (ii) to detect invA gene by PCR for the confirmation of the isolates, (iii) to analyze isolates for S. Enteritidis, S. Typhi, and S. Typhimurium by PCR based on Sdf I, ViaB, Spy gene sequences, respectively, and (iv) to identify isolates in comparison to the reference strain by DNA sequence analysis. A total of 81 unpackaged cream cake samples were obtained from different patisseries in Hatay, Turkey. Salmonella spp. was detected in 13 (16%) out of 81 cream cake samples by the conventional culture method. Atotal of 45 isolates from the 13 positive samples were confirmed as Salmonella spp. by PCR. Homology among the reference strain and isolates and homology within the isolates was found to be 98.97-100%. Cream cake samples analyzed in this study were found to be contaminated with Salmonella, thus posing a potential health hazard for the consumer. To protect public health, food safety management systems such as HACCP, GMP and GHP could be carried out in cream cake production.

3

Effect of some herbicides on the survival of bacteria indicating sanitary conditions and of enteropathogens in lake water. The influence of Afalon

84%

Niewolak S., Komar M.

Natural Sciences

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2001

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tom 08

101-110

4

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Evaluation of Vibrio cholerae as vector for the expression on foreign vaccine antigens

67%

Favre D., Viret J. F.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

5

Effect of some herbicides on the survival of bacteria indicating the sanitary conditions and of enteropathogens in lake water. III. The influence of pielik

67%

Niewolak S., Komar M.

Polish Journal of Natural Sciences

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2002

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tom 10

6

Fe2 - initiated chemiluminescence in rats with high hemoglobin-oxygen affinity during fever

67%

Zinchuk V., Borisiuk M. V.

Journal of Physiology and Pharmacology

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1997

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tom 48

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nr 1

7

Immune responses to phospholipase-A fractions of Salmonella typhi in experimental typhoid infection in mice

67%

Punj V., Matta H., Kanwar S. S.

Acta Microbiologica Polonica

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1996

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tom 45

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nr 3-4

8

Factors affecting intra and extracellular phospholipase A production by Salmonella typhi

59%

Punj V., Matta H., Kalra M. S.