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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers

100%
Marcotuli I., Mazzeo A., Nigro D., Giove L., Giancaspro A., Colasuonno P., Prgomet Z., Prgomet I., Tarantino A., Ferrara G., Gadaleta A.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 4
Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers. A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively. The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies).
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Genetic characterization and analysis of the population of selected Sarawak rice cultivars using simple sequence repeat markers

100%
Razak S. A., Saidon S. A., Yusof M. F. M., Ismail S. N.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2018
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tom 99
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nr 3
3

Searching for the most useful genotypes of Lupinus mutabilis sweet for breeding purpose

84%
Galek R., Kozak B., Sawicka-Sienkiewicz E., Zalewski D., Nowosad K.
Electronic Journal of Polish Agricultural Universities. Series Agronomy
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2017
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tom 20
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nr 4
4

Genetic variation of Salmo trutta L. populations from the catchment areas of the Rega, Parseta and Wieprza rivers evaluated by RAPD and SSR markers

84%
Achrem M., Skuza L., Kirczuk L., Domagala J., Pilecka-Rapacz M., Czerniawski R.
Folia Biologica
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2015
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tom 63
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nr 1
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Identification of four new est-based markers on the apple (Malus × domestica) genetic map

84%
Keller-Przybylkowicz S., Korbin M.
Journal of Horticultural Research
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2014
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tom 22
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nr 1
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The effectiveness of DArT markers conversion to PCR markers useful for the discrimination of Polish barley genotypes with higher spring drought tolerance

84%
Fiust A., Rapacz M., Tyrka M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Maximizing hybrid seedlings recovery and early identification of highly polyembryonic acid lime (Citrus aurantifolia Swing.) × lemon (Citrus limon Burm.) hybrids using SSR markers

84%
Dubey A. K., Gupta A., Sharma R. M., Sharma N.
Journal of Horticultural Research
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2020
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tom 28
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nr 2
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Genetic diversity of Brassica rapa germplasm of Khyber Pakhtunkhwa Pakistan revealed by molecular markers

84%
Ali N., Ali S., Khan N. U., Jan S. A., Rabbani M. A., Hussain I.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 6
A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Genetic diversity and relationship of Hunan province of China local tree peonies based on SSR markers

84%
Zhang M.-H., Jin X.-L., Wen Y.-F., Shen S., Wen-Xing, Wu S., Lu J.-H., Ye-Ye
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 4
Paeonia sect. Moutan is a wide known ornamental plant in the world. The objective of this study was to provide the theoretical basis for scientific preservation and utilization of tree peony resources of Hunan province of China. Simple sequence repeat (SSR) markers were applied to reveal the genetic diversity and relationship of 21 tree peony resources and 45 domestic and foreign tree peony cultivars. Clear bands, the size of which ranged from 115 bp to 379 bp, were detected with 14 primers. In total, 90 alleles were detected and the number of alleles detected with one primer varied between 5 and 13; the number of effective alleles ranged from 1.183 to 2.070; the polymorphism ratio of each locus was 100%. The observed heterozygosity, which ranged from 0.120 to 0.851 with an average of 0.532, was larger than the expected one, which ranged from 0.090 to 0.470 with an average of 0.300. Shannon index ranged from 0.137 to 0.695 and fixation index ranged from −0.332 to −0.869. The results show abundant genetic diversity in tree peony of Hunan province and SSR markers distinguishing homonymous tree peony resources successfully.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Evaluation of genetic diversity of local-colored rice landraces using SSR markers

84%
Thi Hue H., Nghia L. T., Minh H. T., Anh L. H., Thi Thu Trang L., Khanh T. D.
International Letters of Natural Sciences
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2018
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tom 67
Analysis of genetic diversity of 90 Vietnamese local-colored rice accessions was carried out using 40 SSR markers. The numbers of polymorphic alleles ranged from 3 to 12 alleles per locus and average of 7.1 alleles per locus. The similarity coefficients of the rice landraces fluctuated from 0.76 to 0.93; at a genetic correlation level of 0.78. Ninety accessions of rice landraces were divided into five groups based on analysis of genetic relationships. The results have indicated that 11 markers included M250, RM302, RM10926, RM208, RM227, RM17231, RM23251, RM5647, RM1376, RM339 and RM228 which gave the unique allele. These markers can be used effectively for genetic diversity of colored rice and provided a specific database and useful materials for landraces identification, local germplasm conservation for further colored rice improvement on rice quality via rice breeding programs in Vietnam.
11

Genetic variability of Salmo trutta L. species from the catchment areas of the Drawa and Rega rivers evaluated using RAPD and SSR markers

67%
Skuza L., Achrem M., Pilecka-Rapacz M., Czerniawski R., Domagala J., Kirczuk L.
Polish Journal of Natural Sciences
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2014
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tom 29
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nr 2
The knowledge of the genetic variability and structure of Salmo trutta population is needed for effective protection of the species and rational management of the resources. A number of marker systems have been introduced to evaluate the genetic variability of trout populations. Among them, the most often used are the RAPD and SSR markers. Both marker systems are classified as type II markers (O’BRIEN 1991, LERCETEAU-KÖHLER and WEISS 2006). In this study, the genetic variability of the Salmo trutta m. fario and Salmo trutta m. trutta populations from the Rega river, and the three watercourses Sitna, Słopica and Bagnica of the Drawa river catchment area, were analysed. One stream, the Chojnówka (located outside the catchments of the above streams), was used as an extra study area. Based on two marker systems, different results were obtained. In the case of RAPD analysis, all loci were polymorphic in all populations. The use of these marker systems permitted the construction of UPGMA similarity trees. The trees revealed a division of the analysed populations into two groups: one group from the Słopica river and the other group from the remaining watercourses. In the second similarity group, two subgroups can be distinguished: one comprising the population of the sea trout from the Rega river and that of the brown trout from the Sitna river (60.7%), and the other consisting of the parr trout populations from the Chojnówka, Bagnica and Sitna (50.3–79.4%). Between the analysed populations, 100% polymorphism was found. The results indicate a high genetic variability of the studied populations. In the case of SSR analysis, 9 microsatellite loci isolated from five trout populations were described. The number of alleles at these loci ranged from 1 to 5 with an average of 2.8 alleles per locus. The expected heterozygosity ranged from 0.07 to 0.66, with an average of 0.35. The results indicate high genetic variation of the populations studied.
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Identifying the grain chalkiness gene using molecular marker techniques in rice (Oryza sativa L.)

67%
Thi Lang N., Ho Truc Giang P., Thi Thu Ha P., Bao Toan T., Anh Phuong T., Chi Buu B.
International Letters of Natural Sciences
|
2017
|
tom 63
Chalkiness is a major constraint on rice production because it is one of the key factors determining grain quality (appearance, processing, milling, storing, eating, and cooking quality) and price. In this study, we conducted grain chalkiness gene identification using co-dominant insertion/deletion (INDEL) markers and SSR marker combination on 50 different varieties. The application results in 7 InDel markers and SSR marker on chromosome 7 were recorded. Three primers, InDel 5, InDel 14 and RM21938, associated with grain chalkiness. For the InDel 5 primer, the amplification product was 100%. Use of primer InDel 5 in detection and evaluation of genotype to the chalkiness trait of rice grain on 50 rice varieties indicated the suitability level with phenotypic evaluation was 86% and the unsuitability level was 14%. For the InDel 14 primer, the amplification products were 100%. The suitability with phenotypic assessment was 84% and the unsuitability was 16%. For the RM21938 primer, the amplification product was 94%. The suitability with phenotypic assessment was 76% and the unsuitability was 24%. Thirteen of the selected varieties had grain chalkiness gene both InDel 5, InDel 14 and RM21938. Total 13 varieties were detected from InDel 5, InDel 14 and RM12938 primer combinations also showed high efficiency of the InDel technique in identifying chalkiness gene in rice grain. A cluster analysis was performed and a dendrogram was constructed which evinced the nature of phylogenetic classification among the genotypes of the varieties. These markers could be used for developing quality of rice in breeding program.
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