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Lipopolysaccharides (LPS) of Rhizobium galegae, a symbiotically nitrogen-fixing species of root-nodule bacteria, were isolated by the phenol-water method from strain HAMBI 1461, the LPS of which resembled enterobacterial smooth type LPS, and from strains HAMBI 1174 and HAMBI 1208, the LPSs of which resembled rough type LPS. The results of PAGE analysis of LPSs, Bio-Gel P2 gel filtration of polysaccharide fractions and the presence of deoxysugars and 4-O-methyl-deoxysugar both in the rough and smooth LPSs suggested that rough LPS contained a short O-antigenic polysaccharide for which we propose the name short O-chain LPS. Accordingly, the smooth LPS is called long O-chain LPS. Despite of the differences in the structure of LPS of R. galegae, all strains were equally effective in nodulating their hosts. The short O-chain LPS of R. galegae showed many features similar to those of phylogenetically related agrobacteria.
A three-year field experiment investigated the effectiveness of symbiosis between extremely extensively used fodder galega (Galega orientalis Lam.) plants and Rhizobium galegae. A bacterial culture was obtained from root nodule bacteria and applied onto seeds prior to sowing. In summer each year, over the maximal biomass development as well as in autumn the weight of the underground and over-the-ground plant parts was measured, including the content and accumulation of basic elements. The effectiveness of symbiosis between inoculated and non-inoculated plants was visible with a more rapid development, twice higher biomass increase over the sowing year, eleven-fold higher over the second research year and fifteen-fold – over the third year. Inoculated plants contained on average by 1% of nitrogen more than non-inoculated, and over the third research year, over the greatest biomass development, accumulated from a few to a few dozen-fold more phosphorus, potassium, magnesium.
The field experiment was carried out between 1994-1998 at the Agricultural Experimental Station of the Academy of Podlasie, Siedlce on the influence of the means of inoculation with Rhizobium galegae and Nostoc of the Galega orientalis (Lam.) seed variety Gale on the content of dry matter, nitrogen, total protein, ash and crude fiber as well as on the yield of this plant. The climatic conditions during the experiment (1994-1998) were not suitable (very low rainfall) for growing this plant which significantly decreased the yield of the dry mass. The highest yield (10.8 t ha-1) was harvested from the object in which the seeds were inoculated by Rhizobium galegae and Nostoc. Plants harvested from this object also had the highest content of nitrogen (30 g kg-1 d.m.) and total protein (187.5 g kg-1 d.m.). The low content of ash 59 g kg-1 d.m. high in crude fiber (385 g . kg-1 d.m.) were caused by the later than normal harvesting of Galega orientalis.
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