This work includes assessment of the three domestic mixed alfalfa cultivars Kometa, Radius, Tula and the three foreign cultivars Marshal, Planet, Symphonie of the sowing alfalfa with regard to the dry matter yields and the total protein content. The highest dry matter yields were collected in the second year of the full utilization and the lowest in the first. The difference between the cultivar of a higher yield Radius and the cultivar of a lower yield Marshal amounted to 7%. The dry matter content during the first year of the utilization reached the value of 56% for the first regrowth, 28% for the second and 16% for the third one. During the second year of the investigations this value amounted to 51, 25 and 24%, respectively. The total protein content was higher in the first year and relatively 11% lower in the second year for each cultivar. Analysis of the regrowths revealed that the third one was characterised with the highest concentration whereas the lowest was found for the first one and the relative difference amounted to 11%.