The life cycle of Pygidiopsis australis sp. n. was reproduced experimentally, starting from cercariae from naturally infected Littoridina castellanosae (Hydrobiidae) collected from artificial ponds in the Zoological Garden in Buenos Aires. Metacercariae were found encysted in the mesenteries, internal organs and body cavity of experimentally exposed Cnesterodon decemmaculatus (Poeciliidae). Adults were obtained experimentally in chicks and mice. The adult could not be distinguished from P. pindoramensis Travassos, 1929; the cercariae are clearly different in the arrangement of penetration glands and in the absence of pigment granules in the body of P. australis sp. n.
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