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The usefulness of dikegulac in propagation of highbush blueberry [Vaccinium corymbosum L.] 'Herbert'

100%

Litwinczuk W., Prokop A.

Journal of Fruit and Ornamental Plant Research

2010

tom 18

nr 2

85-92

The experiments were carried out on highbush blueberry 'Herbert' both on in vitro cultures and plants in vivo. In the case of the in vitro study, the modified Zimmerman and Broome (1980) medium was used. For the first subculture dikegulac was tested at a 0.1-10 mgl-1 concentration together with 2iP (5mgl-1). For the second subculture, dikegulac (1-4mgl-1) was added both into medium supplemented with 2iP (10 mgl-1), and into medium without 2iP. In the case of the in vivo study, dikegulac (100-1000 mg l-1) was applied as a foliar spray on four-month old plantlets. Dikegulac (0.1-5 mg l-1) gradually slowed down the elongation of axillary shoots in vitro, in the presence of 2iP at a lower (5 mg l-1) concentration. It also retarded development of adventitious shoots, while proliferation of axillary shoots was unaffected. Cultures grew very slowly when 2iP was omitted regardless of the concentration of the retardant Plants sprayed with dikegulac (1000 mg l-1) solution in vivo developed more lateral shoots which were shorter, and the plants had reduced leaf blades. Cuttings collected from plants treated with retardant rooted better, compared with the control. Dikegulac may be useful to keep the germplasm bank and in propagation of highbush blueberry, both in vitro and through cuttings.

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Development of highbush blueberry [Vaccinium corymbosum hort. non L.] in vitro shoot cultures under the influence of melatonin

100%

Litwinczuk W., Wadas-Boron M.

Acta Scientiarum Polonorum. Hortorum Cultus

2009

tom 08

nr 3

The influence of melatonin (MEL) in comparison with IAA, IBA (all at 5.71 µ M), and auxin-free (0) medium on development of highbush blueberry (Vaccinium corymbosum hort. non L.) ‘Herbert’ in vitro shoot cultures was examined. Depending on the kind of hormone in vitro cultures consisted of various number of axillary (AX) and adventitious (AD) shoots. The influence of melatonin on in vitro cultures was intermediate but more similar to IAA than IBA. Production of axillary shoots on media supplemented with MEL and IAA was comparable and higher than on medium with IBA. In contrast to IAA melatonin reduced development of adventitious shoots. Contrary to IBA-obtained cultures AX shoots grown on ‘IAA’, ‘0’, and ‘MEL’ media resembled AD shoots.

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Hortorum Cultus

1 Journal of Fruit and Ornamental Plant Research

2 Litwinczuk W.

1 Prokop A.

1 Wadas-Boron M.

1 2010

1 2009

Wyniki wyszukiwania

The usefulness of dikegulac in propagation of highbush blueberry [Vaccinium corymbosum L.] 'Herbert'

Development of highbush blueberry [Vaccinium corymbosum hort. non L.] in vitro shoot cultures under the influence of melatonin