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An association between Helicobacter infection and autoimmune hepatitis (AIH) in children was investigated. The prevalence of antibodies to H. pylori did not differ between the AIH and the control group, (22% versus 14%), and antibodies to non-gastric Helicobacter were not detected in either group. H. pylori DNA was found in two AIH liver tissues, but Helicobacter was not cultured from any sample.
The aim of the study was to estimate the occurrence, density, and characteristics of inflammatory infiltrations in swine with Helicobacter sp., and particularly Candidatus Helicobacter suis (CHS) infections. Gastric samples from 29 pigs were obtained from slaughterhouses. Helicobacer genus-specific and CHS-specific primers sets were used to determine the Helicobacter status in all animals, and density and characteristics of inflammatory infiltration were estimated during microscope examinations. Helicobacter infections were observed in all the examined cases, and gastritis was noted in most cases. Mononuclear cells (lymphocytes and plasma cells) mostly dominated in the inflammatory infiltrations, and neutrophils were observed more seldom. It was noted that pigs which had Helicobacter infections in this part of the gastric mucosa, also had more intensive inflammatory infiltrations than animals without infection. No differences in the density of inflammatory infiltrations in the body and pyloric antrum of the stomach were noted.
The aim of this study was the evaluation of gastric epithelial cell proliferation rate in connection with the gastric Helicobacter-like organisms (GHLO) infection in dogs. The study was performed on samples of the gastric mucosa obtained during necropsies from 60 dogs euthanasized for various reasons. Microscopic preparations were stained with the hematoxylin-eosin method, Giemsa method, Warthin-Starry silver method and immunohistochemical method (MIB-1 antibody) to investigate the expression Ki67 antigen. In glands of the gastric body and antrum different proliferation patterns were observed. The gastric epithelial cell proliferation rate in the gastric antrum was significantly higher in dogs with GHLO colonization in relation to dogs without GHLO, and in the gastric body was significantly higher in dogs with mild GHLO colonization as compared to dogs with significant GHLO colonization and without GHLO colonization. The gastric epithelial cell proliferation rate in the gastric body was significantly higher in dogs with gastritis in comparision with the dogs without gastritis irrespectively from the presence or absence of the GHLO colonization. It seems that the presence of the GHLO colonization, as well as the inflammatory state in the gastric mucosae, can alter the rate of the gastric epithelial cells proliferation in dogs.
The aim of the study was to evaluate the frequency of the presence of antibodies to Helicobacter pylori and Helicobacter felis antigens in dogs. One hundred thirty two dogs of different age and sex were included in the study and divided in two groups. Serum samples and gastric biopsy specimens were taken during gastrointestinal endoscopy. The examined group consisted of 102 dogs with dyspeptic symptoms and pathological changes in gastric mucosa. The control group contained 30 clinically healthy animals. In the examined group Helicobacter spp. infection was established by direct microscopy and urease test. It was detected in 89.2% of the dogs, whereas in the control group, in 96.7% animals. In the culture of gastric biopsy specimens Helicobacter felis was the most frequently isolated strain. The anti-H. pylori IgG antibodies were detected in 24.5% serum samples of the examined dogs. The anti-H. felis IgG antibodies were identified in 30.4% samples of animals with gastritis and in 20% of healthy animals respectively. Antibodies of H. pylori antigens were not evaluated in healthy dogs. The antibodies to H. pylori and H. felis antigens were detected more frequently in dogs with gastritis than in healthy dogs.
Since the discovery of Helicobacter pylori and the subsequent establishment of its key role in gastritis and peptic ulcer disease in humans, many studies have been undertaken to determine the occurrence of helicobacter in various animal species. As a result, new species of Helicobacter, including Helicobacter heilmannii, have been discovered. Helicobacter heilmannii prevalence in humans is lower than 1%. Helicobacter heilmannii has recently been distinguished into four subspecies (types). Helicobacter heilmannii has also been discovered in the stomachs of dogs, cats and pigs. Candidatus Helicobacter suis, a new species of helicobacter, was found in pigs having 80% of gastric mucosa specimens with ulcerative lesions. A homology of 99.5% has been ascertained in the 16S rRNA gene sequence between Candidatus Helicobacter suis and Helicobacter heilmannii type 1. At present the most important studies in Helicobacter infections in pigs are those relating to the possibility of the animals being naturally infected with Helicobacter pylori and, as a result, transmitting Candidatus Helicobacter suis infections to humans.
Literature data indicate an association between the presence of Helicobacter spp. in the liver and the development of hepatocellular carcinoma (HCC). However, the role of H. pylori infections in chronic liver diseases (CLD) remains controversial. The aim of this study was to detect Helicobacter spp. DNA in patients with CLD, and to investigate the host response to the presence of the bacterium in the liver. Helicobacter spp. DNA was detected in 59% samples. H.pylori was the most prevalent species (94%). We estimated the expression level of IL-1 and IL-8 genes. The presence of Helicobacter spp. did not have a significant effect on the gene expression of IL-8 and IL-1.
The aim of the present study was to evaluate the effect of Candidatus Helicobacter suis (CHS) and other Helicobacter sp. different from Candidatus Helicobacter suis (non-Candidatus Helicobacter suis, non-CHS) infection on the number of endocrine G and D cells and G/D cells ratio in antral gastric mucosa in swine. Twenty nine stomachs were obtained from clinical healthy pigs about 6 months old and weighing approximately 100-120 kg after slaughter at abattoir located in central Poland. From each stomach samples of the antral gastric mucosa were taken for histopathology, and PCR examination for presence of Helicobacter genus and Candidatus Helicobacter suis. Samples for histopathology and immunohistochemistry were fixed in 10% buffered formalin. To reveal the expression of gastrin- and somatostatin-producing cells specific antibodies were used. Selected endocrine cells were counted in the midzone of pyloric glands, the results were expressed as a mean of the number of immunoreactive cells in one microscopic field, and as the ratio of gastrin to somatostatin cells (G/D). It can be concluded that some species of swine Helicobacter can alter the number of endocrine cells in gastric antral mucosa. Some of these alterations, for example increase the number of G cells, decrease of the D cells and especially increase of ratio G to D cells can be responsible for development of gastroesophageal ulcers in swine.
Specimens of gastric mucosa of 17 free-ranging wild boars (Sus scrofa) shot in the Central Poland during 2007/2008 hunting season were investigated for the presence of Helicobacter species. Histopathology, Helicobacter genus-specific 16S rRNA PCR, and DNA sequence analysis were employed. In PCR analysis the presence of Helicobacter's DNA was detected in one stomach. Obtained sequence analysis showed its relatedness to Helicobacter heilmannii type 2. In histopathology of the PCR-positive sample the presence of tightly coiled spiral bacteria was detected on the surface of the antral mucosa, in gastric pits and lumen of the upper parts of antral glands. Potential pathologic significance of the presence of Helicobacter in the stomach of free-ranging wild boars was obscured by the parasitic invasion-caused gastritis, and remains unknown.
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