Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 14

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  HL-60 cell
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid

100%
Kodaira Y., Kusumoto T., Takahashi T., Matsumura Y., Miyagi Y., Okamoto K., Shidoji Y., Sagami H.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 4
777-782
Geranylgeranoic acid (GGA) and 2,3-dihydrogeranylgeranoic acid (2,3-diGGA) are geranylgerani-ol-derived metabolites (Kodaira et al.(2002) J Biochem132:327–334). In the present study, we examined the effects of these acids on HL-60 cells. The cells were differentiated into neutrophils by GGA stimulation like retinoic acid stimulation. In the case of cells stimulated with 2,3-diGGA, neutrophils were not detected, but the formation of lipid droplets was induced. On the other hand, when the cells were cultured in the presence of 0.1% FBS instead of 10% FBS, apoptotic cells were induced not only by GGA stimulation but also with 2,3-diGGA. In the latter case, when the cells were cultured in the co-presence of a caspase-3 inhibitor (Ac-DMQD-CHO), the lipid droplets formation was observed in the cells. These results suggest that GGA and 2,3-diGGA are extremely different from each other with respect to their effects on HL-60 cells.
2

Cytotoxic effects of cladribine and tezacitabine toward HL-60

100%
Stachnik K., Grieb P., Skierski J. S.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 2
The aim of the study was to determine the relation between the cytotoxic and cytostatic effects of tezacitabine and cladribine on a HL-60 cell line and the time of exposure of cells to these drugs. Cell viability and induction of apoptosis were assessed using flow cytometry methods. Apoptosis was confirmed by direct microscopic observation. Growth inhibition was examined by cell counting. After 24 h incubation tezacitabine was equally or less toxic compared to cladribine. However, toxicity of tezacitabine strongly rose after 48 h incubation leading to massive cell death at doses much lower than those of cladribine. Assessment of the effect of increased exposure time on the clinical efficacy of tezacitabine is indicated.
3

Cytological changes in HL-60 cells after treatment with an extract from Rhodiola rosea rhizomes

100%
Majewska A., Hoser G., Furmanowa M., Urbanska N., Kuras M.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
4

Effect of differentiating agents [all-trans retinoic acid and phorbol 12-myristate 13-acetate] on drug sensitivity of HL60 and NB4 cells in vitro

84%
Jasek E., Mirecka J., Litwin J. A.
Folia Histochemica et Cytobiologica
|
2008
|
tom 46
|
nr 3
323-330
5

The antioxidant quercetin protects HL-60 cells with high myeloperoxidase activity against pro-oxidative and apoptotic effects of etoposide

84%
Papiez M. A., Krzysciak W.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 4
6
Content available remote

The wine polyphenol resveratrol modulates autophagy and induces apoptosis in MOLT-4 and HL-60 human leukemia cells

84%
Siedlecka-Kroplewska K., Wozniak M., Kmiec Z.
Journal of Physiology and Pharmacology
|
2019
|
tom 70
|
nr 6
7

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X-100

84%
Panasiewicz M., Domek H., Hoser G., Fedoryszak N., Kawalec M., Pacuszka T.
Cellular and Molecular Biology Letters
|
2009
|
tom 14
|
nr 2
175-189
Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)
8

The importance of abrogation of G2-phase arrest in combined effect of TRAIL and ionizing radiation

84%
Rezacova M., Vavrova J., Vokurkova D., Tichy A., Knizak J., Psutka J.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 4
Background: In this work we studied the relationship between the enhanced expression of DR5 receptor and the effect of combination of TRAIL and ionizing radiation on cell cycle arrest and apoptosis induction in human leukemia cell line HL-60. Material and methods: DR5, APO2.7 and cell cycle were analyzed by flow cytometry. Proteins Bid and Mcl-1 were analyzed by Western-blotting. For clonogenic survival, colony assay on methylcellulose was used. Results: Ionizing radiation caused significantly enhanced positivity of DR5 receptors 24 h after irradiation with high doses (6 and 8 Gy). An increase of DR5 receptor positivity after a dose of 2 Gy was not statistically significant and application of TRAIL 48 h after irradiation did not increase the apoptosis induction. However, a decrease of radiation-induced G2 phase arrest and an increase of apoptosis were observed when TRAIL was applied 16 h before irradiation with the dose of 2 Gy. Incubation with 6 µg/l TRAIL for 16 h reduced D0 value from 2.9 Gy to 1.5 Gy. The induction of apoptosis by TRAIL was accompanied by Bid cleavage and a decrease of antiapoptotic Mcl-1 16 h after incubation with TRAIL. Conclusion: TRAIL in concentration of 6 µg/l applied 16 h before irradiation by the dose of 1.5 Gy caused the death of 63% of clonogenic tumor cells, similarly as the dose of 2.9 Gy alone, which is in good correlation with the enhanced apoptosis induction.
9

In vitro response of human pathological hematopoietic cells to cladribine

67%
Mazur L., Opydo-Chanek M., Stojak M., Janota B., Blicharski K., Wojcieszek K., Klaput U., Borowicz P.
Folia Biologica
|
2013
|
tom 61
|
nr 3-4
10
Content available remote

Pterostilbene induces accumulation of autophagic vacuoles followed by cell death in HL60 human leukemia cells

67%
Siedlewska-Kroplewska K., Jozwik A., Boguslawski W., Wozniak M., Zauszkiewicz-Pawlak A., Spodnik J. H., Rychlowski M., Kmiec Z.
Journal of Physiology and Pharmacology
|
2013
|
tom 64
|
nr 5
11

In vitro effects of new generation oxazaphosphorines on human promyelocytic leukemia cells

67%
Mazur L., Opydo-Chanek M., Stojak M., Niemeyer U.
Folia Biologica
|
2013
|
tom 61
|
nr 1-2
12

Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells

67%
Savickiene J., Treigyte G., Borutinskaite V.-V., Navakauskiene R.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 4
DNMT inhibitors are promising new drugs for cancer therapies. In this study, we have observed the antileukemic action of two diverse DNMT inhibitors, the nucleoside agent zebularine and the non-nucleoside agent RG108, in human promyelocytic leukemia (PML) HL-60 cells. Zebularine but not RG108 caused dose- and time-dependent cell growth inhibition and induction of apoptosis. However, co-treatment with either drug at a non-toxic dose and all trans retinoic acid (RA) reinforced differentiation to granulocytes, while 24 or 48 h-pretreatment with zebularine or RG108 followed by RA alone or in the presence of HDAC inhibitors (sodium phenyl butyrate or BML-210) significantly accelerated and enhanced cell maturation to granulocytes. This occurs in parallel with the expression of a surface biomarker, CD11b, and early changes in histone H4 acetylation and histone H3K4me3 methylation. The application of both drugs to HL-60 cells in continuous or sequential fashion decreased DNMT1 expression, and induced E-cadherin promoter demethylation and reactivation at both the mRNA and the protein levels in association with the induction of granulocytic differentiation. The results confirmed the utility of zebularine and RG108 in combinations with RA and HDAC inhibitors to reinforce differentiation effects in promyelocytic leukemia.
13

Side-chain modified vitamin D analogs require activation of both PI 3-K and erk1,2 signal transduction pathways to induce differentiation of human promyelocytic leukemia cells

67%
Marcinkowska E., Kutner A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 2
Synthetic analogs of vitamin D for potential use in differentiation therapy should se­lectively regulate genes necessary for differentiation without inducing any perturba­tions in calcium homeostasis. PRI-1906, an analog of vitamin D2, and PRI-2191, an analog of vitamin D3 bind nuclear vitamin D receptor (nVDR) with substantially lower affinity than 1,25-dihydroxyvitamin D3 (1,25-D3), but have higher differentiation-in­ducing activity as estimated in HL-60 leukemia cell model. To examine how their in­creased differentiation-inducing activity is regulated we tested the hypothesis that membrane-mediated events, unrelated to nVDR, take part in the differentiation in re­sponse to PRI-1906 and PRI-2191. The induction of leukemia cell differentiation in response to the analogs of vitamin D was inhibited by LY294002 (phosphatidyl- inositol 3-kinase inhibitor), PD98059 (inhibitor of MEK1,2, an upstream regulator of extracellular-signal regulated kinase) and rapamycin (p70 S6K inhibitor) pointing out that activation of signal transduction pathways unrelated to nVDR is necessary for differentiation. On the other hand, inhibition of cytosolic phospholipase A2 acceler­ated the differentiation of HL-60 cells induced by either 1,25-D3 or by the vitamin D analogs suggesting possible existence of a feedback loop between extracellular-signal regulated kinases and phospholipase A2.
14

Wplyw menadionu, metotreksatu, cisplatyny i kwasu okadejowego na komorki bialaczki szpikowej HL-60 in vitro

59%
Rozalski M., Krajewska U.
Bromatologia i Chemia Toksykologiczna
|
1997
|
tom 30
|
nr 3
283-284
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.