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The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.
This study addressed the feeding of sexually mature perch (Perca fluviatilis L.) in Lake Kortowskie in the autumn-winter period (October-March). A total of 81 perch specimens were caught, of which 20.2% were males, 69.1% females, and 7 specimens were of unidentified sex. The composition of the perch diet was very diversified. The food contained zooplankton, insect larvae, fish, and other components. Zooplankton was the most significant in late autumn (54.9% frequency of occurrence), whereas fish and insect larvae were found to dominate in winter. Differences in feeding were observed between the sexes (there was a lack of fish in the male diet) and between specimens of different length classes (specimens with lengths over 20 cm were obligatory predators).
Background. Achtheres percarum is an important copepod parasite (Crustacea: Copepoda) of European perch. Adult females permanently attach to the gill arches, roof of the mouth, tongue, and gill filaments. Attachment, at the latter site, may result in necrosis and epithelial hypertrophy, both compromising fish respiration during oxygen deficiencies. Adult males can move freely on gills of perch. To date there has been no published record of the complete set of developmental stages of this fish parasite. Provision of such may have practical implications for freshwater ichthyopathology, for example helping to monitor the dynamics of the parasite’s populations. It may also provide useful information regarding copepod phylogenetics. Materials and Methods. Early developmental stages (nauplius and copepodid) of A. percarum were acquired through incubation of eggs within egg sacs of females collected from European perch, Perca fluviatilis L., caught commercially in 1994 in Lake Dąbie, Szczecin, Poland. All subsequent larval stages were collected from gills of perch caught in the same lake, in 1990. All copepods were fixed and preserved in 75% ethanol. A modified "wooden slide" method was used to observe the collected developmental stages in a suspended drop of lactic acid, using a compound microscope. Specimens were stained in lignin pink and morphologic details of were drawn using a drawing tube. Results. The life cycle of A. percarum consists of 7 developmental stages, separated by moults (nauplius, copepodid, chalimus I, chalimus II, chalimus III, chalimus IV, and adult). The nauplius hatches from the egg and quickly moults into the copepodid. Both stages are free swimming and the copepodid is the infective stage, attaching to the host′s gill filaments, through the frontal filament. The subsequent chalimus stages (I through IV) "inherit" the copepodid′s frontal filament, modifying its proximal end, such that the structure of the proximal end of the frontal filament explicitly identifies the stage of a chalimus. Two adult males were found attaching, by means of claws of its maxillipeds, to the frontal filament, left over by previous stages. Conclusion. The number of developmental stages of A. percarum determined within the presently reported study is consistent with that hitherto found in the life cycles of other lernaeopodids.
Background. After their deposition in water, eggs of perch form characteristic, long gelatinous ribbons (strands) floating within the water column. The ultrastructure of the external gelatinious egg envelope of members of the family Percidae was believed to be amorphous. The aim of the presently reported study was to describe the ultrastructure and function of the gelatinous egg envelope composing the perch egg ribbon. Materials and Methods. Fresh eggs of European perch, Perca fluviatilis L., were collected, in the form of egg ribbons, from females caught at the spawning grounds on the Odra River side canal Dziewoklicz, within the city limits of Szczecin, Poland. After fertilization eggs were fixed in 4%formaldehyde. In the laboratory, the commonly accepted techniques (dehydration, critical-point drying, sputter coating) were used to prepare samples for examination with a scanning electron microscope (SEM). The observations were photographically documented. Results. Closely below the surface of the gelatinous egg ribbon we observed a network of microtubules. These microtubules opened at the egg ribbon surface in the form of ring-like or nozzle-like pores. Conclusion. The gelatinous egg envelope of perch is a complex microtubular network that likely performs a skeletal role for the egg ribbon. Our results also suggest that the regular surface openings of the aforementioned network may play an attachment function (micro-suckers) reciprocally between eggs and between eggs and other objects, thereby facilitating the fastening the eggs to underwater vegetation.
Proper multidimensional management of perch, common and one of the most abundant species in European freshwaters, is a challenge both from ecological and commercial perspective. However, all activity relating to the management of the perch must be based on good genetic knowledge in order to ensure a purity of species and further conservation of biodiversity. Therefore, in the present study, the mtDNA cytb and D–loop restriction analysis was applied in order to estimate genetic diversity within and among three perch (Perca fluviatilis L.) populations in reservoirs of Central Poland: Sulejów, Próba and Jeziorsko. Two out of nine, and two out of eight restriction enzymes were polymorphic for cytb and D–loop, respectively. Five different haplotypes were detected with the simultaneous occurrence of haplotype 1 in all three populations. Nucleotide diversity values within investigated populations were low (0.003 to 0.02). No significant differentiation (P = 0.248) among populations representing two different catchments of Pilica and Vistula rivers, was observed. The lack of genetic differentiation of perch populations is the first important element for the development of a strategy to regulate the perch in Polish river systems, with simultaneous reduction of fish in eutrophic reservoirs and increase in amount of perch, in sites favorable for fishing and aquaculture.
Communities of parasites of European perch from lake Łebsko were studied and compared with similar communities from the Polish coastal zone. Parasites comprised 18 autogenic and 5 allogenic species. Most individual parasites belonged to allogenic species and were in larval stages. The majority of specimens were eye parasites with Tylodelphys clavata as the eudominant species. The dominant species, Acanthocephalus lucii, belongs to the intestine parasite community. Three marine species were found: Bothriocephalus scorpii, Hysterothylacium aduncum and Echinorchynhus gadi. The results indicate that the parasite fauna consists mostly of freshwater species, common in various types of European waters, while marine species were rarely observed.
The total mercury content was determined in the muscle of northern pike, perch, and roach caught in four lakes (Łańskie, Pluszne, Dłużek, Maróz) in the Olsztyn Lake District (northeastern Poland) from October 1999 to October 2000. The total mercury was analyzed with flameless cold vapor atomic absorption spectrometry (CV AAS). The total mercury content in the muscle of pike ranged from 0.076 mg kg⁻¹ (Łańskie) to 0.902 mg kg⁻¹ (Pluszne), in perch from 0.104 mg kg⁻¹ (Pluszne) to 1.277 mg kg⁻¹ (Dłużek), and in roach from 0.074 mg kg⁻¹ (Maróz) to 0.278 mg kg⁻¹ (Maróz). In most cases, the strongest positive correlation (p < 0.001) was found between the Hg concentration in the muscle tissue of the fish and total body weight (0.825 < r < 0.967) or total body length (0.781 < r < 0.950). The exception was the roach from Lake Maróz, where the correlation coefficients between mercury content in the muscle of these fish and their body weight and total length were r = 0.794 and r = 0.788, respectively (p < 0.01). Similarly, with pike from Lake Maróz it was found that the correlation coefficient between the mercury concentration in the muscle of these fish and their total length was r = 0.781 (p < 0.01).
Background. The study was carried out in the shallow, eutrophic Oświn Lake, in the area of the Seven Islands ornithological reserve (north-eastern Poland) to determine the infection of fish with digeneans maturing in piscivorous birds. This paper presents a comparative analysis of the occurrence of Posthodiplostomum cuticola in fish from two parts of the reservoir differing in environmental conditions. The effect of fish size and season of sampling on the infection level was analysed. Materials and Methods. Within 1998 and 1999, a total of 1091 fishes of 8 species dominating in the lake were examined. Samples were collected four times a year (in May, July, August, and October) simultaneously from the two examined parts of the lake. Results. Metacercariae of P. cuticola occurred with a high prevalence and intensity of infection in roach, rudd, white bream, and carp bream, and sporadically in crucian carp and European perch. The intensity of infection of fish from the western part of the lake was significantly higher compared with the eastern part; roach from the western pool were much more infected than in the other reservoirs of north Poland. The infection of roach, white bream and carp bream increased with total body length (TL), such a relationship was not reported for rudd. The infection of roach, white bream and carp bream varied significantly in different experimental periods while differences in the infection of rudd in samples were incidental. Conclusion. Location-dependent occurrence of P. cuticola in fishes indicates that interchange between fish groups from the eastern and western pool of Oświn Lake is limited. Infection of rudd was of a more incidental character then the infection of roach, white bream, and carp bream.
Przedstawiono wyniki analiz pozostałości chlorowych pochodnych węglowodorów HCB, HCH, DDT z metabolitami oraz PCB w tkance mięśniowej i wątrobach trzech gatunków ryb odłowionych w Zalewie Szczecińskim.
Background. Considerable amounts of fish bones and scales were discovered in 1953-1963, in an archaeological excavation pit situated in a former Vegetable Market in Szczecin, on the left bank of River Odra. Materials and Methods. Bone remains comprising 725 labelled collections from various dated sediment layers were identified. Individual bones were compared to those of corresponding extant fish species from water bodies near Szczecin and were identified to bone type and assigned to species. The scales were identified as well. Results. A total of 10 085 (76.2%) bone remains, out of 13 229, could be identified. They belonged to 20 fish species. Most abundant were remains of carp bream (Abramis brama), zander (Sander lucioperca), roach (Rutilus rutilus), tench (Tinca tinca), wels catfish (Silurus glanis), European perch (Perca fluviatilis), northern pike (Esox lucius), and sturgeon (Acipenser sturio). Conclusion. The majority of fish species targeted by early-mediaeval fishermen are also very important in the present-day fisheries in the area. It can be presumed that intensive sturgeon fishery in early Middle Ages markedly contributed to the species′ extinction from the area.
Perch (Perca fluviatilis) netted in the Gdańsk Gulf in 1987 and 1989, was searched for Cu, Mn, Zn, Fe, Ca, Mg, Na, and K content in the muscular tissue and liver, using atomic absorption or emission spectrometry. Average concentrations (mg/kg wet weight), were found as follows: Cu - 0.26, Mn - 0.31, Zn - 7.7, Fe - 2.9, Mg - 290, Ca - 390, Na - 1000, adn K - 5700. As the body weight of perch increased, Mn, Fe, Zn, and Ca concentrations in the muscular tissue and Mn content in the liver, were observed to decrease. On the other hand, Cu, Na, K, and Mg concentrations in muscles and Cu, Fe, and Zn levels in the liver, remained constant. A positive intermetallic linear correlation was show for the following pairs of metals: Cu-Fe, Mn-Fe, Mn-Zn, and Fe-Zn.
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