The aim of this study was to determine the impact of sex on the content of β-carotene, lutein, astaxanthin, canthaxanthin, zeaxanthin, and total carotenoids in the skin, muscles, and livers of whitefish (Coregonus lavaretus lavaretus (L.)). Fish samples for assays were collected from Lake Łebsko. There is a whitefish conservation program in Lake Łebsko. The results show that males are more carotenoid rich in comparison to females. The total carotenoids ranged from 0.39 μg g⁻¹ wet weight (ww) (skin of females) to 1.55 μg g⁻¹ ww (livers of males). The highest carotenoid levels were in whitefish fivers (1.36 - 1.55 μg g⁻¹ ww), and the lowest were in its skin (0.39 - 0.41 μg g⁻¹ ww). Astaxanthin was the dominant carotenoid in whitefish muscles and fivers. The highest levels of β-carotene were recorded in the skin of both whitefish males and females.