Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: Candidatus Phytoplasma asteris

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ subgroup rpI-L in Iran

100%

Vali-Sichani F., Bahar M., Zirak L.

Journal of Plant Protection Research

2014

tom 54

nr 2

In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.

Detection of Russian olive witches’-broom disease and its insect vector in Northwestern Iran

100%

Hajizadeh A., Khakvar R., Bashir N. S., Zirak L.

Journal of Plant Protection Research

2017

tom 57

nr 3

Recently, Russian olive trees showing witches’-broom and little leaf symptoms have been widely observed in northwestern and central Iran. Polymerase chain reaction (PCR) and nested PCR assays using phytoplasma universal primer pairs confirmed phytoplasma symptomatic infection of trees. Sequence analyses showed that ‘Candidatus Phytoplasma asteris’ was the causal agent of the disease in these regions. However, RFLP results using restriction enzymes HpaII, EcoRI, HinfI and AluI indicated that the collected isolates in these regions are genetically different. In addition, leafhopper Macropsis infuscata was recognized as a possible insect vector of the disease for the first time.

Ograniczanie wyników

2 Journal of Plant Protection Research

2 Zirak L.

1 Bahar M.

1 Bashir N. S.

1 Hajizadeh A.

1 Khakvar R.

1 Vali-Sichani F.

1 2017

1 2014

Wyniki wyszukiwania

Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ subgroup rpI-L in Iran

Detection of Russian olive witches’-broom disease and its insect vector in Northwestern Iran