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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Survival rate of Suid Herpesvirus (SuHV-1, Aujeszky`s disease virus, ADV) in composted sewage sludge

100%
Paluszak Z., Lipowski A., Ligocka A.
Polish Journal of Veterinary Sciences
|
2012
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tom 15
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nr 1
Sewage sludge constitutes a source of valuable biogenic raw materials, but it is a carrier of many pathogenic microorganisms and viruses. Subjected to an effective sanitization by means of the process of composting, it is suitable to use in agriculture as fertilizers. The aim of this study was to observe the survival rate of Suid Herpesvirus under the influence of the temperature alone (water bath) as well as in sewage sludge subjected to the process of composting (pile). The samples were taken at different time intervals, and the virus titres were determined. The viruses survived considerably longer under laboratory conditions: at 30°C as long as 21 days, at 40°C - 93 hours, and at 50°C - less than an hour. In the compost pile, in spite of the lack of the thermophylic phase, the total survival time of the viruses ranged from 34 to 44.5 hours, which indicates the vast importance of other physicochemical factors, apart from the temperature, contributing to virus inactivation.
2

Effect of KLP-602 on virus replication in cell cultures

84%
Malaczewska J., Rotkiewicz Z.
Polish Journal of Veterinary Sciences
|
2004
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tom 07
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nr 2
3

Humoral response and changes in IL-2 receptor's expression after immunisation of piglets with maternal antibodies against Aujeszky's disease

84%
Markowska-Daniel I., Pomorska-Mol M., Bednarek D.
Bulletin of the Veterinary Institute in Puławy
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2009
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tom 53
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nr 2
169-174
The aim of the study was the evaluation of immunological response of piglets born from sows immunised with ADV live vaccine and defining the optimal time for their active immunisation. Two weeks after the final vaccination, there were no differences in the level of serum gB antibodies among the sows and all the sows developed humoural immunity. Maternally derived antibodies (MDA) in the sera of all piglets were above the level considered to be positive until about 11 weeks of their life. Following exposure to the live ADV, a greater number of lymphocytes (including CD2⁺ and CD2⁻ cells) from vaccinated animals expressed the IL-2 receptor (CD25), than those from unvaccinated ones. After virus stimulation, there were also higher expression of CD25 on CD8⁺ T cells in all vaccinated animals, while in non-vaccinated pigs; a decrease in such expression was evident. Based on the obtained results it might be stated that the piglet vaccination against AD at 10 and 14 weeks of life was considered to be optimal. In this age, the animals were still protected by passive immunity, but simultaneously were able to develop an active humoural response. It could be also concluded that the high level of MDA may successfully blocked the developmental of active immunity.
4

Effect of methisoprinol on virus replication in cell cultures

84%
Malaczewska J., Rotkiewicz Z.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 2
5

Pathomorphological studies on organs of piglets immunized with two vaccines Suivac A and Geskyvac and infected with Aujeszky’s disease virus

84%
Rotkiewicz T., Siemionek J., Ruta A., Rotkiewicz Z.
Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria
|
1990
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tom 19
6

Biological properties of Roakin strain of NDV and TK900 strain of ADV after serial passages in CECC in the presence of Methisoprinol and KLP-602

84%
Malaczewska J., Rotkiewicz Z.
Polish Journal of Veterinary Sciences
|
2005
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tom 08
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nr 1
7

Rozprzestrzenienie wirusa choroby Aujeszkyego w populacji swin w wojewodztwie olsztynskim

67%
Szweda W., Grzechnik R., Bronicka A.
Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria
|
1995
|
tom 22
78-85
Celem badań była kompleksowa ocena sytuacji epizootycznej choroby Aujeszkyego (chA) w fermach państwowych i prywatnych jako podstawa wdrożenia programu zwalczania choroby w woj. olsztyńskim. Badania wykonano w 159 fermach państwowych i 121 prywatnych, co stanowiło odpowiednio 80.7% i 80.1% wszystkich ferm w woj. olsztyńskim. Zbadano 19 294 surowice świń (18 167 z ferm państwowych i 1 127 z prywatnych) używając zestawów do testu ELISA (Bioveta-Ivanovice). Wykazano, że średni współczynnik zapowietrzenia ferm państwowych, będący stosun­kiem liczby ferm zapowietrzonych do ogólnej liczby ferm, wynosił w woj. olsztyńskim 57.0%. Średni współczynnik zakażenia stad świń w fermach państwowych, będący stosunkiem liczby świń zakażonych do ogólnej liczby świń w stadzie, określono w woj. olsztyńskim na 70.4%, z wahaniami od 2.8% do 100.0% w poszczególnych stadach. Świnie w fermach prywatnych w woj. olsztyńskim są generalnie wolne od wirusa chA (1 knur zakażony na 1127 świń badanych został natychmiast wyeliminowany). Sytuacja epizootyczna chA wskazuje na potrzebę włączenia jej do wykazu chorób objętych obowiązkiem zgłaszania i rozpoczęcia akcji zwalczania zgodnie z wymogami europejskimi.
8
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Survival of BPV and Aujeszky's disease viruses in meat wastes subjected to different sanitization processes

67%
Paluszak Z., Lipowski A., Ligocka A.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 4
The effect of composting and anaerobic fermentations under meso- and thermophylic conditions (37° and 55℃) on the survival of bovine parvovirus (BPV) and Aujeszky’s disease viruse (ADV) in meat wastes has been examined in this study. Viruses were adsorbed on filters and introduced into carriers which were made of meat fragments of different sizes and bones or in the form of suspension they were introduced into the biomass in the course of processes of waste treatment. Carriers were removed at appropriate time intervals and virus titres were determined. The thermoresistant parvovirus survived for the longest time during mesophylic fermentation (almost 70 days), slightly shorter during composting (7-9.5 days depending on the type of carrier) and for the shortest time – at 55℃ (46-76 hours). Its inactivation rate was the fastest in a suspension, slower in meat and bone carriers. ADV inactivation proceeded considerably faster, as compared with BPV. Its active particles were not detected as early as in the 30th minute of thermophylic fermentation, the 6th hour of mesophylic fermentation and at the first sampling time during composting (at the 72nd hour). Total survival time ranged from 50 min to 13 hours. All the tested technologies enabled the effective elimination of ADV and on average twofold decrease in BPV titre. From the study conducted it follows that of both viruses, the BPV should be applied for validation processes of methods used in meat waste processing, particularly if this refers to methods where higher temperature is the factor inactivating pathogens.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Amplification of DNA from bovine herpesvirus type 1

67%
Rola J.
Bulletin of the Veterinary Institute in Puławy
|
1997
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tom 41
|
nr 1
The aim of the study was to adapt PCR for detection of BHV 1 after in vitro multiplication. Primers were synthesised for a fragment of the gIV glycoprotein gene of the reference Cooper strain of BHV 1. Polish BHV 1 isolates obtained in the 1970s from bull semen were examined. A positive amplification occurred for the strains belonging to subtype BHV 1.2 and subtype BHV1.1. No amplification has occurred with the DNA of EHV 1 and Aujeszky’s disease virus, which confirmed the specificity of the primers used. The specificity of amplification was proved by digestion of the PCR products with Alu I, Ava I, Bgl I, and Hind III restriction enzymes. The electrophoretic pattern of the PCR products digested with these enzymes was in conformity with the restriction map of the amplified fragment.
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