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The influence of mercury and cadmium on the activities of some enzymes from Siberian sturgeon [Acipenser baeri Brandt 1869] semen

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Sarosiek B., Zdanio G., Kowalski R. K., Glogowski J.

Polish Journal of Natural Sciences

2007

tom 22

nr 1

137-149

The effects of mercury and cadmium ions, at the concentrations of 100, 10 and 1 mg · dm⁻³, on the activity of enzymes contained in the sperm of Siberian sturgeon were determined. Whole semen was frozen without a cryoprotector. Acid phosphatase (AcP) activity decreased significantly following the application of mercury and cadmium ions at the highest concentration. Lactic dehydrogenase (LDH) was found to be the most sensitive to metal ions of all enzymes examined in the study. The concentration of 10 mg · dm⁻³ almost completely inhibited the activity of this enzyme. It was also found that mercury and cadmium ions slightly stimulated the activity of arylsulfatase (AS). The activity of β -N-acetylglucosaminidase (β -NAG) decreased considerably when mercury ions were added to the reaction mixture, in contrast to cadmium ions. The effects of Hg and Cd ions on the affinity of AcP and AS for substrates were also determined. It was found that these metal ions decreased the affinity of the above enzymes for p-nitrophenylphosphate and p-nitrocatechol sulfate respectively.

Ograniczanie wyników

1 Polish Journal of Natural Sciences

1 Glogowski J.

1 Kowalski R. K.

1 Sarosiek B.

1 Zdanio G.

1 2007

Wyniki wyszukiwania

The influence of mercury and cadmium on the activities of some enzymes from Siberian sturgeon [Acipenser baeri Brandt 1869] semen