The presence of turkey Coronavirus (TCoV) was monitored in meat-type turkey flocks in Poland between 2008 and 2009. Clinical samples (10 individual faecal swabs/flock) from turkeys, aged from 7 d to 19 weeks, from 136 flocks were collected from different regions of the country. TCoV detection was accomplished by molecular techniques. The prevalence of Coronavirus in 7.3% of tested flocks was found. To investigate the virus shedding and its presence in the intestines, liver, kidneys, and bursa of Fabricius of infected SPF turkeys and contact chickens, the quantitative real time reverse transcription-polymerase chain reaction was applied. The presence of virus in turkey swabs from 5 to 14 d post infection and also in intestines and bursa of Fabricius was detected. Surprisingly, contact chickens revealed to be sensitive to TCoV infection.