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The association of brain derived neurotrophic factor gene variants with behaviour traits in sika deer (Cervus nippon)

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Lv S.-J., Yan Y., Wei W.-H.

Journal of Animal and Feed Sciences

2012

tom 21

nr 3

It is widely accepted that brain-derived neurotrophic factor (BDNF) is involved in modulating behaviour performance induced by environmental conditions. The aim of this study was to study polymorphisms of the BDNF gene and their relationship with animal behaviour in sika deer (Cervus nippon). Forty-eight sika deer reared at the Ping-Shan-Tang Farm (25 deer) and Zhu-Yu-Wan Park (23 deer), Yangzhou City, Jiangsu Province (China) were observed and blood samples were taken to identify BDNF genotypes. Data were subjected to ANOVA analysis to evaluate the link between genotype and animal behaviour traits. After PCR and electrophoresis, polymorphisms were found in two pairs of primers. At primer P-4, the AA genotype (26 deer) rested significantly less than the BB genotype (16 deer) (P<0.05). The AA genotype deer also exhibited significantly more locomotor behaviour (P=0.001). At primer P-5, deer of genotypes CC/DD/CD differed significantly in their watching behaviour. Deer of genotype CC exhibited significantly less resting and self-grooming behaviour than deer of genotypes CD or DD (both P<0.05). Our findings suggest that polymorphisms in BDNF may be involved in some aspects of animal behaviour traits, especially in the highly sensitive sika deer reared for several years in Chinese parks.

Polymorphisms of the interleukin-15 gene and their associations with fatness and muscle fiber traits in chickens

76%

Lv S.-J., Su L., Li H., Han R.-L., Sun G.-R., Kang X. T.

Journal of Applied Genetics

2012

tom 53

nr 4

Interleukin-15 (IL-15) is a cytokine that has been proposed to modulate skeletal muscle and adipose tissue mass. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken IL-15 gene. Two single nucleotide polymorphisms (SNPs) (g.31224G>A and g.31266T>G) were identified in exon 5 of the IL-15 gene by means of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. Associations between the two SNPs and chicken fatness and muscle fiber traits were determined using linkage disequilibrium, haplotype construction, and association analysis. Both of the SNPs were associated with abdominal fat weight, leg muscle fiber diameter, and leg muscle fiber density (p < 0.05). Haplotypes of the two linked SNPs were associated with abdominal fat weight, fat thickness under the skin, and leg muscle fiber diameter (p < 0.05). The results suggested that the IL-15 gene might be associated with the causative mutation or the quantitative trait locus (QTL) controlling the fatness traits and muscle fiber traits in chickens.

Ograniczanie wyników

1 Journal of Animal and Feed Sciences

1 Journal of Applied Genetics

2 Lv S.-J.

1 Han R.-L.

1 Kang X. T.

1 Li H.

1 Su L.

1 Sun G.-R.

1 Wei W.-H.

1 Yan Y.

2 2012

Wyniki wyszukiwania

The association of brain derived neurotrophic factor gene variants with behaviour traits in sika deer (Cervus nippon)

Polymorphisms of the interleukin-15 gene and their associations with fatness and muscle fiber traits in chickens