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Use of mass spectrometry for characterising microbial communities in bioaerosols

100%

Szponar B., Larsson L.

Annals of Agricultural and Environmental Medicine

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2001

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tom 08

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nr 2

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Levels of bacterial endotoxin in the samples of settled dust collected in animal houses

71%

Pomorska D., Larsson L., Skorska C., Sitkowska J., Dutkiewicz J.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 1

37-41

Samples of settled dust were collected in 14 animal houses and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based on the detection of 3- hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of the endotoxin in dust determined with LAL test in sheep sheds, poultry houses, and horse stables were 15,687.5 µg/g, 8,081.8 µg/g, and 79.3 µg/g, respectively, while those determined with the GC-MSMS technique were 868.0 µg/g, 580.0 µg/g, and 496.0 µg/g, respectively. Statistical comparison of the results yielded with LAL test and GC-MSMS technique revealed a weak correlation between both methods. Fatty acids with 14-16 carbon chains (3-OH-C₁₄ and 3-OH-C₁₆) were predominant in the settled dust of the facilities under study. In conclusion, endotoxin in the concentrations detected in this study may present a respiratory hazard to both livestock animals and farm workers. Thus, the prevention measures aiming to lower the exposure to endotoxin in livestock facilities are highly desirable.

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Levels of bacterial endotoxin in air of animal houses determined with the use of gas chromatography - mass spectrometry and Limulus test

71%

Pomorska D., Larsson L., Skorska C., Sitkowska J., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2007

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tom 14

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nr 2

Air samples were collected on glass fi bre fi lters in 22 animal houses and 3 hay storage barns and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography – tandem mass spectrometry (GC-MSMS) technique, based on detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of airborne endotoxin determined with LAL test in poultry houses, sheep sheds, piggeries, cow barns, and horse stables were respectively 62.49 μg/m3, 26.2 μg/m3, 3.8 μg/m3, 1.65 μg/m3, and 1.14 μg/m3, while those determined with the GC-MSMS technique were respectively 1.06 μg/m3, 7.91 μg/m3, 0.2 μg/m3, 0.31 μg/m3, and 1.42 μg/m3. The median concentrations of airborne endotoxin determined with LAL test and GC-MSMS technique in hay storage barns were much smaller, 0.09 μg/m3 and 0.03 μg/m3, respectively. The concentrations of airborne endotoxin (LPS) detected with GC-MSMS method in the air of sheep sheds were signifi cantly greater than in all other examined facilities, while those detected in hay storage barns were signifi cantly smaller than in all other examined facilities (p<0.05). The concentrations of airborne endotoxin determined with LAL test and GC-MSMS analysis exceeded in most of animal houses examined (91% by each method) the threshold limit value for airborne endotoxin of 0.1 μg/m3 proposed by various authors. A signifi cant correlation (p<0.05) between the concentrations of endotoxin determined with the LAL and GC-MSMS techniques was found in the air samples collected in poultry houses and sheep sheds, but not in other examined facilities. 3-OH FAs with C14-C18 chains were predominant in the air of the facilities under study. A signifi cant correlation (p<0.05) was found between the concentrations of endotoxin determined with LAL test and the amounts of 3-OH FAs with C14-C16 chains. In conclusion, endotoxin in the concentrations detected in this study may present a respiratory hazard to both humans and livestock animals.

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Assessment of microbial exposure risks from handling of biofuel wood chips and straw - effect of outdoor storage

71%

Sebastian A., Madsen A. M., Martensson L., Pomorska D., Larsson L.

Annals of Agricultural and Environmental Medicine

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2006

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tom 13

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nr 1

Handling of biofuels may release dust particles containing high concentrations of hazardous microorganisms, thus representing a potential occupational health problem. We analysed the microbial dustiness of baled straw (cultivated both conventionally and ecologically) and of wood chips from piles that had been stored outdoors for up to 11 months by using total spore counting, cultivation, and measuring of endotoxin and chemical markers of fungal biomass, lipopolysaccharide, and peptidoglycan. The bacterial dustiness of straw was much greater than of wood chips whereas the fungal dustiness did not differ much. In general, samples taken from the inner part of each biofuel material were dustier than samples taken from the surface, except for fungal and bacterial biomass in wood chips and total fungi and fungal biomass in ecological straw. A considerable increase of bacterial dustiness occurred during storage over summer. Dust from ecological straw contained considerably less of bacterial components than from conventional straw and, in addition, exhibited a less pronounced increase upon storage over summer. In summary, biofuels represent sustainable energy resources of growing economic importance but may at the same time pose significant health problems. We found that storage of biofuels outdoors over summer increased the microbiological dustiness and should therefore be avoided, and that ecological straw contained less of microbe-containing dust than conventional straw and should be preferred since it reduces the exposure to harmful microbiological agents.

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Routine clinical laboratory tests correspond to increased serum levels of 3-hydroxy fatty acids, markers of endotoxins, in cardiosurgery patients

71%

Krasnik L., Szponar B., Walczak M., Larsson L., Gamian A.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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tom 54

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nr 1

6

Endotoxin exposure in sewage treatment workers: investigation of exposure variability and comparison of analytical techniques

61%

Spaan S., Smit L. A. M., Eduard W., Larsson L., Arts H. J. J. M., Wouters I. M., Heederik D. J. J.

Annals of Agricultural and Environmental Medicine

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2008

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tom 15

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nr 2

7

Occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in Poland

42%

Gora A., Mackiewicz B., Krawczyk P., Golec M., Skorska C., Sitkowska J., Cholewa G., Larsson L., Jarosz M., Wojcik-Fatla A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2009

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tom 16

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nr 1

143-150

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Respiratory disorders in two workers of customs depositories occupationally exposed to mouldy tobacco

42%

Mackiewicz B., Skorska C., Krysinska-Traczyk E., Larsson L., Szponar B., Milanowski J., Czekajska-Chebab E., Sitkowska J., Cholewa G., Szymanska J., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2008

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tom 15

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nr 2

Ograniczanie wyników

Use of mass spectrometry for characterising microbial communities in bioaerosols

Levels of bacterial endotoxin in the samples of settled dust collected in animal houses

Levels of bacterial endotoxin in air of animal houses determined with the use of gas chromatography - mass spectrometry and Limulus test

Assessment of microbial exposure risks from handling of biofuel wood chips and straw - effect of outdoor storage

Routine clinical laboratory tests correspond to increased serum levels of 3-hydroxy fatty acids, markers of endotoxins, in cardiosurgery patients

Endotoxin exposure in sewage treatment workers: investigation of exposure variability and comparison of analytical techniques

Occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in Poland

Respiratory disorders in two workers of customs depositories occupationally exposed to mouldy tobacco