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Fragments of chromatograms recorded before aspartic acid during amino acid analysis of hydrolysates of plant materials are compiled. On such chromatograms, peaks corresponding to cysteic and levulinic acids, methionine sulfoxide and several unidentified coumpouns are recorded. The number of unidentified peaks depends on the kind of plant material analysed.
Physical training can be classified into three main types: endurance, resistance, and patterned movements. The first two of them have a significant impact on muscle phenotype and metabolism while patterned movement exercises concern changes in a motor program in the central nervous system and result in only slight changes in muscle tissue. Adaptation to endurance versus resistance training in most aspects is extremely different. Due to the mutually opposite nature, in classical training systems, endurance and resistance exercises are very often separated. Nowadays, in sport as well as recreation and rehabilitation it is postulated to combine both types of exercises. Because of this, the very important question arises as to how combined workouts including strength and endurance exercises will affect the body. An even more important question concerns the proportions of both types of exercises, their intensity and duration. Therefore, defining safe and effective training systems can be beneficial not only for athletes but also for the prevention of civilization-related diseases and aging effect.
Introduction. Physical performance displays a great interindividual variance in both general population and among well-rained athletes. Genetic factor has an important contribution in this variance. The aim of this study was to examine the association between maximal oxygen uptake and genetic variants of mitochondrial NADH dehydrogenase subunit 5 gene (mtND5) in Caucasians from Poland. Material and Methods. The studies were carried out in a group of 154 men and 85 women, professional athletes representing various sports and fi tness levels and students of the University of Physical Education in Poznań. Physiological and molecular procedures were used, i.e. direct measurement of maximal oxygen uptake (VO₂max) and SNP 13470 G>C polymorphism of the mitochondrial NADH dehydrogenase subunit 5 gene (mtND5) was determined by restriction fragments length polymorphism (PCR-RFLP). Results. We have found that maximal oxygen uptake is associated with BamHI+/+ homoplasmic variant of the mtND5 gene in Caucasians from Poland. We have also observed positive infl uence of BamHI+ allele on level of maximal oxygen uptake (VO₂max).
 Uncoupling proteins 2 and 3 (UCP2 and UCP3) as mitochondrial electron transporters are involved in regulation of ATP production and energy dissipation as heat. Energy efficiency plays an important role in physical performance, especially in aerobic fitness. The aim of this study was to examine the association between maximal oxygen uptake and genetic variants of the UCP2 and UCP3 genes. The studies were carried out in a group of 154 men and 85 women, professional athletes representing various sports and fitness levels and students of the University of Physical Education in Poznań. Physiological and molecular procedures were used, i.e. direct measurement of maximum oxygen uptake (VO2max) and analysis of an insertion/deletion (I/D) polymorphism in the 3'untranslated region of exon 8 of the UCP2 gene and a C>T substitution in exon 5 (Y210Y) of the UCP3 gene. No statistically significant associations were found, only certain trends. Insertion allele (I) of the I/D UCP2 and the T allele of the UCP3 gene were favourable in obtaining higher VO2max level and might be considered as endurance-related alleles.
Introduction. 110-m and 400-m hurdles races are based on anaerobic energy metabolism which can induce muscle fatigue and muscle fiber damage. The most common biochemical parameters used in controlling athletes’ training loads and post-exercise fatigue are blood lactate (La) concentration and creatine kinase (CK) activity. Aim of Study. The aim of the study was to determine and compare runners’ biochemical response after 110-m and 400-m hurdles races. The influence of warm-up before both races was taken into consideration. Material and Methods. Eight male hurdlers took part in this research. They were subjected to two test exercises: a 110-m and a 400-m hurdles race. During each test, pre-warm-up, post-warm-up, and post-exercise capillary blood from a fingertip was collected to determine the La concentration and CK activity. Furthermore, during both sprint runs the athletes’ time (to the nearest 0.01 s) and heart rate (HR) were measured. Results. Each kind of exercise test increased the La concentration and CK activity. More significant changes of both biochemical parameters occurred after the 400-m race. Furthermore, after each warm-up significant increases of La and CK levels were observed. After the 400-m hurdles race higher HR values were noted (184.50 ± 8.32 compared to 177.50 ± 11.14 after the 110-m sprint). Conclusions. Both specialist warm-up and 110 and 400 meters hurdles races lead to significant changes in athletes’ physiological and biochemical blood parameters. La concentration and CK activity demonstrate greater muscle fatigue and muscle fiber damage after a 400-m than a 110-m hurdles race.
Introduction. Numerous studies have focused on the association between I/D ACE and physical fi tness; however, this association in professional hockey players has never been recognized. Aim of the Study. The study examined the distribution of Alu insertion (I)/deletion (D) angiotensin converting enzyme (ACE) genotypes in elite male professional fi eld hockey players. Material and Methods. The effect of Alu insertion (I)/deletion (D) angiotensin converting enzyme (ACE) genotypes on motor skills and maximal oxygen uptake (VO₂max) in 47 elite male fi eld hockey players was studied. Genotyping for ACE I/D was performed using a polymerase chain reaction on DNA from leucocytes. The studied motor skills such as speed – 20 m run, power – vertical jump, recovery – step-test, speed endurance (15 x 20 m) shuttle run, were established using functional tests. The VO₂max was measured during progressive exercise test till exhaustion. Results. The authors do not confi rm some literature data that D allele favours endurance ability. We did not detect signifi cant genotype effects of ACE on the analyzed traits; how-ever a tendency for decreased performance of individuals with the DD genotype was noted for vertical jump, power peak and power. Conclusions. Analysis of the genetic profi le of ACE I/D may provide supplemetary information on a player’s predispositions to exercise with specifi c energy requirements.
Introduction. Cervical spinal cord injury is one of most common human body deficiencies. Quadriplegia affects not only the mass of paralyzed muscles, but also disrupts physiological exercise adaptation mechanisms. One of the few sports suitable for individuals with cervical spine impairment is wheelchair rugby. Professional athletes display a higher exercise capacity than untrained people with a similar degree of spinal damage. The reduction of aerobic capacity in individuals with cervical spinal cord injury is multifactorial. In addition to cardio-pulmonary mechanisms limiting the exercise capacity, the decrease in active muscle mass leads to the rapid development of tissue hypoxia. Material and Methods. 14 members of the Polish National Wheelchair Rugby Team were recruited for the study. The male players aged 20-40 years with cervical spinal cord injuries underwent spirometric and ergospirometric tests. Results. The mean values of spirometric parameters were: VC 3.9 ± 0.71 l (71.3% predicted), ERV 0.9 ± 0.33 l (60.7% predicted), VE 12.6 ± 6.34 4 l/min, Bf 18.3 ± 4.72 l/min, VT 0.7 ± 0.20 l, FVC 4.05 ± 0.69 l/min (76.3% predicted), FEV₁,₀/FVC 92.2 ± 7.10% (113.45% predicted), MVV 141.7 ± 24.59 l/min (97.15% predicted). The mean value of peak oxygen consumption during exercise was 1.31 ± 0.30 l/min (17.8 ± 4.99 ml/kg/min) achieved within 11.8 ± 3.51 min. The mean maximal workload was 42.5 ± 13.99 W. During the test only 10 players reached the anaerobic threshold (AT) intensity. The mean workload at AT was 36 ± 10.62 W, and VO2 max at AT was 0.9 ± 0.26 l/min (15.5 ± 4.17 ml/kg/min). Conclusions. The results of spirometric tests indicate the presence of mild and medium restrictive pulmonary changes in 8 out of the studied players of the Polish National Wheelchair Rugby Team. The physiological parameters obtained during the exercise test indicate a higher aerobic capacity of surveyed athletes in comparison with untrained quadriplegics.
 Physical fitness is a trait determined by multiple genes, and its genetic basis is modified by numerous environmental factors. The present study examines the effects of the (CA)n tandem repeats polymorphism in IGFI gene and SNP Alw21I restriction site -202 A>C polymorphism in IGF1BP3 on VO2max - a physiological index of aerobic capacity of high heritability. The study sample consisted of 239 (154 male and 85 female) students of the University School of Physical Education in Poznań and athletes practicing various sports, including members of the Polish national team. An association was found between -202 A/C polymorphism of IGFBP3 gene with VO2max in men. Higher VO2max values were attained by men with CC genotype, especially male athletes practicing endurance sports and sports featuring energy metabolism of aerobic/anaerobic character. A statistically significant influence of allele 188 and genotype 188/188 of tandem repeats (CA)n polymorphism of IGF1 gene on VO2max was found in women. Also, lower values of maximal oxygen uptake were noted in individuals with allele 186 or genotype 186/186, and higher VO2max values in athletes with allele 194.
BACKGROUND AND AIMS: Endurance training is based on a repeated, prolonged activation of a large number of muscles. It causes morphological, biochemical and metabolic changes in the muscles and the nervous system. However, there are no data concerning changes in motor unit (MU) contractile properties following endurance training. METHODS: 61 male rats were assigned to 4 groups, untrained – control (C), and 3 groups trained on a treadmill, 5 days a week for 2 weeks (2W), 4 weeks (4W) or 8 weeks (8W). The special protocol determined duration and speed of locomotion in the consecutive days of the training. Finally, rats of the 2W group covered average distance of 5.5 km, 4W of 21 km, and 8W of 56 km. Afterwards, functionally isolated MUs of the medial gastrocnemius muscle were electrophysiologically investigated. RESULTS: The mean body mass of rats of all trained groups was lower in comparison to the C group, but no differences between 2W, 4W and 8W groups were noted. The muscle mass and the muscle-to-body mass ratio were not different between groups. The proportion of fast resistant (FR) MUs was higher, while of fast fatigable (FF) lower in all trained groups, in comparison to the C group. The relative number of slow (S) MUs increased only in the 8W group. MU contractile properties were changed mainly in FR MUs and included: lower contraction and half-relaxation times, lower twitch forces, higher tetanus forces and lower twitch-to-tetanus ratio. Few adaptive changes were noted also for S MUs of trained animals: lower twitch and tetanus forces as well as lower twitch-to-tetanus ratio. For FR and S MUs of all trained groups the force decrease within four minutes of the fatigue test was considerably slower or even completely abolished, which was reflected in the increased fatigue indexes. CONCLUSION: The main adaptive changes appeared early and then slowly increased within the endurance training. Supported by the National Science Center grant 2013/09/B/ NZ7/02555.
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