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Analysis of influences of spaceflight on chemical constituents in licorice by HPLC-ESI-MS/MS

100%

Zhang J.-Z., Gao W.-Y., Gao Y., Liu D.-L., Huang L.-Q.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 6

Focusing on the variations of chemical constituents in licorice root, influences of exposure to physical factors of spaceflight on licorice (Glycyrrhiza uralensis Fisch.) seeds were investigated. Licorice seeds obtained from two different producing areas were flown on a recoverable satellite for 18 days. After returning to earth, the seeds carried by the satellite and the parallel ground control were cultivated to maturity under the same condition. Chromatographic fingerprint of 1 year licorice root analyzed by high performance liquid chromatography with diode-array detection not only displayed the contents of glycyrrhizic acid and liquiritin increasing in the spaceflight samples but showed the variation of the kinds of chemical constituents. The main components in the root extract were identified by high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry. The changes in the kind of secondary metabolites of licorice root after spaceflight were firstly reported. A total of 26 components which included 9 flavonoids, 16 triterpene saponins and 1 coumarin were identified according to their mass spectra determined in both negative and positive ion modes. The research provided the scientific data for spaceflight breeding of medicinal plant and indicated that the technology of spaceflight may be a new effective method for the breeding and cultivation of licorice.

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Advances in study of ginsenoside biosynthesis pathway in Panax ginseng C. A. Meyer

86%

Wang J., Gao W.-Y., Zhang J., Zuo B.-M., Zhang L.-M., Huang L.-Q.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 2

Panax ginseng C. A. Meyer is one of the important nutraceutical and medicinal plants, which is used worldwide. Until now, ginseng has been reported to contain saponins, antioxidants, peptides, polysaccharides, fatty acids, vitamins, alkaloids, lignans, and flavonoids. The saponins, known as ginsenosides, are widely believed to be the major bioactive compounds of ginseng. In this article, ginsenoside biosynthesis pathway and key enzymes regulation are also described. This review provides a reference for improving ginsenoside contents through regulation of ginsenoside biosynthesis pathway.

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Combination effect of lactoalbumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures

86%

Wang J., Gao W. Y., Zhang J., Huang T., Wen T.-T., Huang L.-Q.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 3

Lactoalbumin hydrolysate (LH) at 100 mg L⁻¹ with methyl jasmonate (MJ) at 2 mg L⁻¹ synergistically stimulated ginsenoside accumulation in Panax quinquefolium cells compared with 100 mg L⁻¹ LH. Combination elicitors led to higher ginsenoside productivity (45.93 mg L⁻¹) than single treatment of 100 mg L⁻¹ LH (31.37 mg L⁻¹). This present result will be helpful in providing a tool for enhancing the productivity of ginsenoside by Panax quinquefolium cell cultures on a commercial scale.

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Huang L.-Q.

2 Gao W.-Y.

2 Wang J.

2 Zhang J.

1 Gao W. Y.

1 Gao Y.

1 Huang T.

1 Liu D.-L.

1 Wen T.-T.

1 Zhang J.-Z.

1 Zhang L.-M.

1 Zuo B.-M.

1 2012

2 2011

Analysis of influences of spaceflight on chemical constituents in licorice by HPLC-ESI-MS/MS

Advances in study of ginsenoside biosynthesis pathway in Panax ginseng C. A. Meyer

Combination effect of lactoalbumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures