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1

Morphological changes in duodenal epithelium of Japanese quail after chronic cadmium exposure

100%
Cigankova V., Almasiova V., Holovska K.
Polish Journal of Environmental Studies
|
2010
|
tom 19
|
nr 2
275-282
Our study investigated morphological changes in enterocytes of adult Japanese quails that were given cadmium (CdCl₂) perorally and individually by tube, dissolved in water at a dose of 0.24 mg Cd per animal per day, for 57 and 118 days. The aim of our study was to observe chronic effects of cadmium on the structure of duodenal epithelium by means of light microscopy (LM) and transmission electron microscopy (TEM). On day 57, following peroral administration of cadmium, necrotizing enterocytes were found in the apical part of intestinal villi and their occurrence was only sporadic. Particularly on day 118 following cadmium administration, we were able to observe clusters of 2-3 necrotizing cells in the apical part of intestinal villi. However, the structure and ultrastructure of goblet cells was normal. The most notable finding in ultrastructure of all enterocytes of treated animals was the damage to cell organelles. Mitochondria and cisternae of the endoplasmic reticulum were more or less damaged and the cytoplasm contained flocculent material, particularly in the basal part of enterocytes. Some enterocytes exhibited signs of necrosis, shrivelled nucleus and damaged organelles within the markedly electrondense cytoplasm. Microvilli on the apical surface of these enterocytes were damaged and disintegrated. Junctions between cells of the intestinal epithelium were disturbed, and a present of intracellular plaques associated with the adhering and occluding junctions was observed. Cadmium caused the formation of gaps within the specialized junctional complexes, and injury to enterocytes results in the breakdown of the intercellular attachments and the sloughing of the injured cells into the intestinal lumen.
2

Morphological changes in the liver and the response of antioxidant enzymes after turkeys’ chronic exposure to cadmium

100%
Holovska K., Sobekova A., Almasiova V., Cigankova V.
Polish Journal of Environmental Studies
|
2013
|
tom 22
|
nr 5
We observed the effect of cadmium (Cd group) and cadmium+zinc (Cd/Zn group) in the liver of turkeys after a 71-day exposure. The experimental birds were divided to three groups; control group (Control), group exposed to cadmium (group Cd) as CdCl₂ (aqueous solution, 0.5 mg/kg feed); and group Cd/Zn exposed to the same dose of cadmium as Cd group plus zinc as ZnSO₄ (aqueous solution, 90 mg/kg feed). Light and electron microscopy revealed pronounced changes in the liver of turkeys from the Cd group, such as hyperaemia, dilatation of sinusoids and accumulation of inflammatory cells, including macrophages, heterophils, and lymphocytes in sinusoids. Necrotizing hepatocytes were observed sporadically. The ultrastructural changes included swollen mitochondria with injured cristae, dilated cisternae of rough endoplasmic reticulum, and damaged intercellular contacts between hepatocytes. In the Cd/Zn group, Zn was not able to completely protect the liver, but the changes were less pronounced. The specific activity of superoxide dismutase was significantly increased in the Cd group. Glutathione peroxidase was significantly increased in all experimental groups. In the Cd/Zn group, zinc co-administration had a protective effect on the activity of antioxidant enzymes. Exposure of turkeys to cadmium did not affect the content of TBARS in the liver.
3

Densitometric analysis of alkaline phosphatase in the duodenal and jejunal enterocytes of 8-week-old piglets with retarded growth

100%
Cigankova V., Neuschl J., Bugarsky A., Takacova D.
Bulletin of the Veterinary Institute in Puławy
|
2002
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tom 46
|
nr 1
4

Effect of enterocin a on the intestinal epithelium of Japanese quails infected by Salmonella Duesseldorf

100%
Cigankova V., Laukova A., Guba P., Nemcova R.
Bulletin of the Veterinary Institute in Puławy
|
2004
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tom 48
|
nr 1
5

Effect of long-term experimental hypodynamy on the adrenal glands of Japanese quails: an ultrastructural study

100%
Cigankova V., Zibrin M., Boda K., Holovska K.,Jr.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 4
6

Disorder of sexual development in a Yorkshire terrier (78, XY; SRY-positive)

88%
Dianovsky J., Holeckova B., Hajurka J., Sivikova K., Cigankova V.
Journal of Applied Genetics
|
2013
|
tom 54
|
nr 2
A 9-month-old Yorkshire terrier was admitted to the clinic because of abnormal sexual behaviour and clitoral hypertrophy. External examination confirmed standard development of caudal genital organs: vagina, vulva and cervix uteri. Serum profile of gonadotropin hormones 17 β- estradiol (<10.0 pg.ml−1) and testosterone (9.1 ng.ml−1) revealed the presence of testicular tissue. A midline laparotomy was performed to detect the cranial parts of the genital system. Gonads resembling testicles, structures indicating epididymis and rudimentary deferent ducts were resected, along with adherent part of the uterus. Cytogenetic analysis showed a male chromosomal complement 78, XY in all metaphases of the studied Yorkshire terrier dog. The chromosomal constitution was confirmed by fluorescence in situ hybridisation (FISH) with whole-chromosome painting probes specific for chromosomes X and Y, as well as by polymerase chain reaction (PCR) amplification of the 271- bp Y-linked fragment of SRY (the sex-determining region on the Y chromosome) gene. Sequencing of the dog’s SRY gene coding region did not reveal any mutation. To search for potential mutation in the SOX9 gene (Sry-box containing gene 9), which is considered to be one of the key genes involved in the sex determination process, the PCR fragments of exons 1, 2 and 3 originating from the canine patient were sequenced in order to compare with both male and female healthy control dogs. In the analysed regions of the SOX9 gene, no mutation was found.
7

Effect of hypodynamy on structure and enzymatic activity of duodenal enterocytes of Japanese quail

76%
Cigankova V., Zibrin M., Lenhardt L., Almasiova V., Holovska K., Skrobanek P.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 1
The effect of hypodynamy on the structure, ultrastructure, and enzymatic activity of the duodenal enterocytes of Japanese quail (Coturnix coturnix japonica) was studied. On the second day after hatching, 42 chicks were placed in individual slings suspended by a flexible metal device in such a manner that their legs could not touch the floor. Feed and water was provided ad libitum. The experimental animals were euthanized at 5, 7, 14, 21, 28, 35, and 42 d of age. The birds of control group were kept under standard conditions on the floor. Samples of the duodenum were routinely processed for a light microscope (LM) and also a transmission electron microscope (TEM), along with the determination for alkaline phosphatase (AP) activity. Morphological changes in enterocytes of chicks reared under hypodynamy from day 2 after hatching were observed on days 5 and 7 of age. LM showed that the apical ends of intestinal villi contained clumps of necrotizing enterocytes, which were more numerous compared to that of the control. TEM revealed a loss of microvilli and changes in both the nucleus and cytoplasm in these cells. Besides considerable damage to mitochondria, they exhibited also typical vesiculation of cytoplasm and damaged intercellular junctions. From day 14 to 42 of age morphological changes were less pronounced. On day 7 of age, the activity of AP in the microvilli of duodenal enterocytes of experimental chicks was increased in comparison with the control (P<0.001). On day 14 of the experiment, AP in the investigated structures showed only a slight increase (P<0.05), and on day 21 was also slightly increased (P<0.01). On days 28, 35, and 42 of age, no significant differences in the activity of AP were observed. Hypodynamy acted as a stress factor on Japanese quail, which was stronger at the beginning of the experiment. The animals gradually adapted to their respective conditions. Our results show that Japanese quail chicks are capable of feed consumption and feed conversion under conditions of hypodynamy.
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