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The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen

100%

Akarsu S. A., Acısu T. C., Gungor I. H., Cakır Cihangiroolu A., Koca R. H., Turk G., Sonmez M., Gur S., Fırat F., Esmer Duruel H. E.

Polish Journal of Veterinary Sciences

2023

tom 26

nr 1

The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 μM, LUT 50 μM, LUT 100 μM and LUT 200 μM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 106 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 μM compared to the other groups (p<0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 μM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H2O2 percentage was found to be lower in all experimental groups compared to the control group (p<0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.

Ameliorative effects of ganoderma lucidum (Reishi) on testicular tissue of rats exposed to bisphenol A

100%

Acısu T. C., Akarsu S. A., Sonmez M., Yuce A., Ceribası S., Dayan Cinkara S., Ozer Kaya S., Turk G., Gur S.

Polish Journal of Veterinary Sciences

2024

tom 27

nr 3

Bisphenol A (BPA), an endocrine disrupting chemical, is an environmental toxicant widely used in the production of polycarbonate plastics, epoxy resins and paints. Ganoderma lucidum (GDL) is a plant with biological activities widely used in Chinese medicine. The present study aims to determine the effects of GDL against testicular dysfunction in rats exposed to BPA. For this purpose, a total of 24 Sprague Dawley rats, 6 rats in each group, were used in the study Rats were administered 25 mg/kg/bw BPA and 300 mg/kg/bw GDL by oral gavage for 8 weeks. After the treatments, the rats were sacrificed, and testicular tissues were removed. One of the testes was used for biochemical analyses and the other for histopathologic examinations. The caudal part of the epididymis was trimmed, and semen was obtained. As a result, BPA increased MDA level in blood and testicular tissue, while it decreased CAT, GPx activity and GSH level. GDL treatment provided protection from the impaired oxidant balance (p<0.001). Furthermore, BPA caused decreased epididymal sperm motility and density, vesicular seminalis weight and blood testosterone levels, increased testicular and epididymal tissue weight (p<0.001). Histopathological examination revealed that BPA caused narrowing in testicular tubules and apoptosis, decreased germinal cell thickness and androgen receptor number. It was determined that GDL administration preserved testicular histology. As a result, it was determined that BPA caused toxicity in the testicular tissue of rats, whereas GDL administration was ameliorative.

Ograniczanie wyników

2 Polish Journal of Veterinary Sciences

2 Acısu T. C.

2 Akarsu S. A.

2 Gur S.

2 Sonmez M.

2 Turk G.

1 Cakır Cihangiroolu A.

1 Ceribası S.

1 Dayan Cinkara S.

1 Esmer Duruel H. E.

1 Fırat F.

1 Gungor I. H.

1 Koca R. H.

1 Ozer Kaya S.

1 Yuce A.

1 2024

1 2023

Wyniki wyszukiwania

The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen

Ameliorative effects of ganoderma lucidum (Reishi) on testicular tissue of rats exposed to bisphenol A