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Effect of milk diet on the selected indicators of immunity of rats. The studies were conduced on 2 groups of the rats (10 animals in each group). Group I received milk diet and group II - synthetic one. The aim of the studies was to state whether the milk diet, and more strictly, biologically active components of milk affected the indicators of resistance of the rats, being the model animals for man. The following indices of immunity were determined: level of leukocytes together with the percentage participation of neu­trophils and basophiles, the content of lymphocytes, the level of immunoglobulins IgG, IgE and IgM and IL-2 and IL-4 and also, the percentage of phagocytic neutrophils and monocytes and their phagocytic activity. Statistically confirmed diffe­rences between the mentioned above indicators of immunity were found only in case of signifi­cantly higher level of lymphocytes in the blood of the rats from the group, receiving milk diet as compared to their content in the blood of the rats, receiving synthetic diet.
The aim of the study was to examine whether extracts from herbal preparation Herbatan and of Echinacea purpurea, being employed as feed additives for dairy cows, and being added directly to milk, affected its functional value. The studies were conducted on Wistar rats, at random classified in 6 groups, 10 animals in each group. The rats from group 1 received milk from the cows which received addition of 150 ml of Herbatan in their diet. The milk for the rats from group 2 derived from the cows fed the diet with the addition of Echinacea purpurea extract (8.6 mg/kg of body weight). In group 3, the animals received 400 μl of Herbatan in their daily ration of milk; in group 4, the rats received the addition of 1.13 ml of Echinacea purpurea extract. The rats in all group received 55 ml of milk per head, in average. The animals of group 5 received milk without herbal extracts and those ones from group 6 were fed the synthetic diet. The following determinations were carried out: body weight, relative body weight of the rats, the selected biochemical parameters in blood serum: TP, ALB, BUN, GLU, TRI, CHOL, HDL, LDL, VLDL, AST and ALT, morphological parameters of red and white cell system, the level of the selected immunological indicators: immunoglobulins IgG, IgE, IgM, Il-2 and Il-4 in blood serum of the rats, phagocytic activity of monocytes and neutrophils of peripheral blood. Histological measurements of intestinal villi were carried out. The effect of herbal extracts on functional value of milk was reflected in: lower content of cholesterol and LDL in the rats fed the milk diet with the direct addition of Herbatan and Echinacea purpurea to the milk as compared to the rats from the remaining groups, in the highest level of erythrocytes and hemoglobin in the rats receiving the milk from the cows fed with the addition of Herbatan, in the intensive fluorescent effect of phagocytic neutrophils in the group of the rats receiving the milk from the cows fed the diet with Herbatan addition and in the group of the rats which received the addition of Echinacea purpurea directly to milk.
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