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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

100%
Tomaszewska-Sowa M.
Herba Polonica
|
2020
|
tom 66
|
nr 4
2

Immunocytochemiczna lokalizacja epitopów pektyn i proteoglikanów AGP w regenerujących tkankach buraka cukrowego (Beta vulgaris L.)

100%
Tomaszewska-Sowa M.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2011
|
nr 259
3

Cytometric analyses of sugar beet (Beta vulgaris L.) plants regenerated from unfertilized ovules cultured in vitro

100%
Tomaszewska-Sowa M.
Electronic Journal of Polish Agricultural Universities. Series Agronomy
|
2010
|
tom 13
|
nr 4
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of growth regulators and other components of culture medium on morphogenesis of sugar beet (Beta vulgaris L.) in unfertilised ovule in vitro cultures

100%
Tomaszewska-Sowa M.
Acta Agrobotanica
|
2012
|
tom 65
|
nr 4
The aim of the present research was to optimise the process of plant regeneration from unfertilised ovules in sugar beet (Beta vulgaris L.). A two-stage method was applied which involved preculture in liquid MS medium and culture on solid media. The study evaluated the effectiveness of different groups of growth regulators, agar and gelrite on the morphogenesis of explants. To do so, the culture was maintained on 21 MS media containing auxins (NAA, IBA, 2,4-D), cytokinins (BAP, KIN, TDZ), antiauxin (TIBA) as well as solidifying substances at different concentrations and combinations. Varied effectiveness of callus and shoot formation was observed depending on the medium used. The highest efficiency of shoot formation was recorded on the media containing 4.4 μM BAP, 0.09 M sucrose, and 0.04% gelrite. The formation of roots was induced when exposed to 14.8 μM IBA and 0.049 μM 2iP, and the percentage of rooted shoots ranged from 0.0 to 65.2%, depending on the growth regulator combination used for shoot regeneration.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The application of nanometal colloids in plant in vitro culture

63%
Bocian K., Tomaszewska-Sowa M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Environmental and ecological aspects of cultivation of selected energy and herbal plants propagated by in vitro culture

63%
Tomaszewska-Sowa M., Figas A.
Infrastruktura i Ekologia Terenów Wiejskich
|
2014
|
nr IV/3
There has been a growing interest over the last years in techniques using alternative and pharmaceutical plants due to their wide potential applications. These species are characterised by valuable and multidirectional usable features, due to which their practical application is superior to their physiognomic features. These plants may provide a raw material for production of medicines, cosmetics or may be used for animal feeds. Moreover they may be used as ornamental, melliferous and energy plants. Owing to their generally low soil requirements they are used in the reclamation of degraded land. Among the species characterized by multidirectional use are Silphium perfoliatum, Helichrysum arenarium and Stevia rebaudiana. These plants are characterized by a high content of active compounds and a number of pro-health characteristics. Because raw materials for production of medicines and cosmetics should be standardized, cultures maintained under controlled conditions are a good source of seedlings.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Optimization of the processes of sterilization and micropropagation of cup plant (Silphium perfoliatum L.) from apical explants of seedlings in in vitro cultures

63%
Tomaszewska-Sowa M., Figas A.
Acta Agrobotanica
|
2011
|
tom 64
|
nr 4
Cup plant (Silphium perfoliatum L.) is a tall, reaching up to 2.5 m, perennial plant that represents the Asteraceae family. Silphium perfoliatum L can be applied in medicine, animal feeding, and as a decorative, honey-producing and energy production plant which proves its multi-functional features. Sowing material currently available in Poland is insufficient, which justifies the present attempts at propagating these plants in in vitro cultures. Therefore, Silphium perfoliatum L. seed sterilization and micropropagation processes were studied under controlled conditions in in vitro cultures. Among the tested methods, ACE proved to be the most effective for sterilization. The apical parts of seedlings were used as explants; they were placed onto MS media with growth regulators added. 4 growth regulator concentration variants were applied. The highest percentage of explants with lateral shoots (41.7%) and callus (90.3%) was obtained on MS medium with 5 mg x dm-3 BAP + 1 mg × dm-3 NAA added. Shoots were isolated and transferred onto MS rooting medium without growth regulators. The rooted plants were transferred to the greenhouse and acclimatised to ex vitro conditions.
8

Plant growth regulators affecting in vitro callus, shoots and roots induction of Silphium perfoliatum l. – a new alternative multifunctional plant

63%
Tomaszewska-Sowa M., Figas A.
Electronic Journal of Polish Agricultural Universities. Series Agronomy
|
2015
|
tom 18
|
nr 1
9

The influence of carbohydrates on the regeneration of the sugar beet shoots [Beta vulgaris L.] in in vitro cultures

63%
Tomaszewska-Sowa M., Majewska-Sawka A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Establishing an efficient explant superficial sterilization protocol for in vitro micropropagation of bear’s garlic (Allium ursinum L.)

51%
Tomaszewska-Sowa M., Figas A., Keutgen N., Keutgen
Herba Polonica
|
2015
|
tom 61
|
nr 4
Allium ursinum L. has a commercial value due to its high contents of bioactive compounds and mild, garlic-like taste. In vitro culture played an important role in obtaining Allium species with the desired characteristics and in the production of healthy reproductive material. Developing an effective method of sterilization bear’s garlic bulbs. To obtain sterile shoots of garlic several methods of sterilization involving such factors as ACE, H2O2, HgCl2, and UV-C were tested. In order to obtain sterile shoots of bear’s garlic, several sterilization procedures were tested. The best procedure was based on a two-step disinfection, where the whole onions were treated with ethanol and H2O2 for 20 min. Thereafter, the isolated apical buds were sterilized in ACE for 10 min, rinsed in double-distilled water and transferred onto MS medium for growing. Up to 95% of the inoculated explants formed shoots, which were sub-cultured on MS with 4 mg dm-3 BAP in order to enable further propagation. After optimization and stabilization, this procedure may become the basic concept of a proper and reliable propagation method of bear’s garlic on commercial scale.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Influence of drip irrigation on the height of the biennial cup plant (Silphium perfoliatum L.) from the micropropagation seedlings

51%
Figas A., Rolbiecki R., Tomaszewska-Sowa M.
Infrastruktura i Ekologia Terenów Wiejskich
|
2015
|
nr III/2
12

Wplyw 6-benzyloaminopuryny i humianu sodu na procesy regeneracji papryki w kulturach in vitro

51%
Tomaszewska-Sowa M., Cegielski R., Drozdowska L.
Zeszyty Naukowe Akademii Techniczno-Rolniczej w Bydgoszczy. Rolnictwo
|
2001
|
tom 46
131-137
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Wpływ nawadniania kroplowego na wzrost rożnika przerośniętego (Silphium perfoliatum L.) uprawianego na glebie bardzo lekkiej z sadzonek uzyskanych w wyniku mikrorozmnażania

51%
Figas A., Rolbiecki R., Tomaszewska-Sowa M.
Infrastruktura i Ekologia Terenów Wiejskich
|
2011
|
nr 10
W doświadczeniu polowym, przeprowadzonym w latach 2009-2010 na glebie bardzo lekkiej w Kruszynie Krajeńskim koło Bydgoszczy, badano wpływ nawadniania kroplowego na wybrane parametry wzrostu rożnika przerośniętego uprawianego z sadzonek uzyskanych w procesie mikrorozmnażania in vitro. Doświadczenie założono jako jednoczynnikowe w układzie zależnym, w czterech powtórzeniach. Czynnikiem stanowiącym źródło zmienności było nawadnianie kroplowe zastosowane w dwóch wariantach: O - bez nawadniania (kontrola), K - nawadnianie kroplowe, przeprowadzane na podstawie wskazań tensjometrów (-0,04 MPa). Materiałem do badań były rośliny rożnika przerośniętego (Silphium perfoliatum L.), które uzyskano w procesie mikrorozmnażania. Nawadnianie kroplowe istotnie wpłynęło na zwiększenie świeżej masy liści z jednej rośliny oraz liczby liści. Zwiększyło istotnie powierzchnię transpiracyjną jak również podwyższyło zawartość suchej masy liści.
14

Genetyczne i fizjologiczne aspekty stosowania nanosrebra w kulturach in vitro. Wpływ nanocząstek na cykl komórkowy i syntezę dna wybranych roślin rolniczych

51%
Bocian K., Sliwinska E., Tomaszewska-Sowa M., Lojko A.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 2
15

Histological analysis of organogenesis and somatic embryogenesis during shoot formation in sugar beet (Beta vulgaris L.) via gynogenesis

51%
Tomaszewska-Sowa M., Figas A., Gatz A.
Polish Journal of Natural Sciences
|
2017
|
tom 32
|
nr 4
The aim of the present research were histological analysis of regenerating structures through in vitro gynogenesis from unfertilized ovules of sugar beet (Beta vulgaris L.). The process of shoot regeneration using a novel two stage method combines the preculture in liquid medium with the culture on solid medium. The highest number of explants that formed shoots (60%) was observed on medium supplemented with BAP, sucrose and gerlite, and as regards carbohydrates used in the medium most of explants forming shoots (42%) and the largest total number of shoots (110) was observed for glucose. To accurately determine the course of shoot formation, histological analyses were performed. Careful histologic evaluation of regenerating structures revealed the presence of numerous meristematic centres. In some meristems formation of specialized tissues and organs was observed, including epidermis, apical meristem, leaf primordia and tracheal elements. The analyses showed that the regeneration of the new structures from sugar beet ovules occurred both through organogenesis as well as somatic embryogenesis since the presence of somatic embryos in the globular stage or torpedo stage were observed.
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

A non caloric sweetener Stevia rebaudiana Bertoni - tissue cultured plantlets for organic farming and home gardening

51%
Tomaszewska-Sowa M., Figas A., Sawilska A. K.
Infrastruktura i Ekologia Terenów Wiejskich
|
2015
|
nr IV/3
17

Assessment of metal nanoparticle-induced morphological and physiological changes in in vitro cultures of rapeseed (Brassica napus L.)

51%
Tomaszewska-Sowa M., Siwik-Ziomek A., Figas A., Bocian K.
Electronic Journal of Polish Agricultural Universities. Series Agronomy
|
2018
|
tom 21
|
nr 4
18
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Improvement of in vitro propagation and acclimation of Helichrysum arenarium L. Moench

51%
Figas A., Tomaszewska-Sowa M., Sawilska A., Keutgen A. J.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2016
|
tom 15
|
nr 4
Explants of apical buds of sandy everlasting Helichrysum arenarium L. Moench sterilized with calcium hypochlorite – Ca(OCl₂) were placed onto Murashige- -Skoog (MS) growth medium enriched with 1 mg∙dm⁻³ KIN (kinetin) in two consecutive passages. To optimize the procedure of in vitro micropropagation, the axillary shoots of Helichrysum arenarium L. Moench were transferred during the third passage onto 9 different combinations of MS medium without plant growth regulators (PGRs-free medium), with different concentrations of KIN (1.0, 3.0, 4.0, 5.0 mg∙dm⁻³) and KIN (1.0, 3.0, 4.0, 5.0 mg∙dm⁻³) with IAA (indole-3-acetic acid) (0.5 mg dm⁻³). The highest mean number of shoots (24.7) was observed on MS medium with 5 mg∙dm⁻³ KIN and 0.5 mg∙dm⁻³ IAA. The achieved branched shoots were rooted and acclimatized. Rhizogenesis was intensified by the presence of growth hormones: 0.5 mg∙dm⁻³ IAA or 0.5 mg∙dm⁻³ IBA (indole-3-butyric acid). At the stage of acclimation of plantlets the application of a water solution of MS salts (25%) for watering the plants increased the efficacy of plant acclimation from 56 to 75%.
19

Mikrorozmnażanie roślin rożnika przerośniętego (Silphium perfoliatum L.) w kulturach in vitro

51%
Figas A., Tomaszewska-Sowa M., Lisiak W.
Ekologia i Technika
|
2010
|
tom 18
|
nr 5
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Assessment of phytoaccumulation of trace elements in medicinal plants from natural habitats

51%
Tomaszewska-Sowa M., Kobierski M., Sawilska A. K., Figas A.
Herba Polonica
|
2018
|
tom 64
|
nr 3
The high concentration of some trace elements in medicinal plants may lowering the value of herbal material, and may cause poisoning effects. The aim of this research was to evaluate the content of trace elements in the organs of: sandy everlasting, yarrow and stinging nettle. In the soil samples, the grain size composition, pH, the content of carbon and nitrogen were determined. In the plant material and for the soil samples, the total contents of Zn, Cu, Mn, Fe, Pb were assayed. The values of the bioconcentration factor and translocation factor were also calculated. The concentration of metals in plant tissues followed in order: Fe>Mn>Zn>Pb>Cu>Hg. The studied species of medicinal plants accumulated mainly Zn, Pb and Hg. It was also shown a high Hg mobility and a low Pb mobility which mainly accumulated in the roots.
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