During the cryopreservation procedure, the effect of three different cryoprotectants: dimethylacetamide (DMA), dimethylformamide (DMF) and dimethylsulfoxide (DMSO) on Green-Legged Partridge semen was evaluated. Semen collected from 18 healthy males was pooled after initial assessment. In fresh semen concentration, motility and morphology were assessed. Within five minutes after collection, ejaculates were diluted with EK diluent, then frozen in straws. After equilibration, statistically significant differences (p < 0.05) were observed in sperm motility (DMA: 82.0%, DMF: 82.5%, DMSO: 65.0%) as well as in the proportions of live (DMA: 81.2%, DMF: 84.0%, DMSO: 68.5%) and dead spermatozoa (DMA: 18.8%, DMF: 16.0%, DMSO: 31.5%) in the case of DMSO when compared to DMA and DMF. Post-thaw sperm motility differed significantly (p < 0.05) for DMSO (23.0%) compared to DMF (39.5%) and DMA (37.5%). Statistically significant differences (p < 0.05) were noted between cryoprotectants in terms of the proportion of live (DMA: 37.9%, DMF: 47.1%, DMSO: 30.8%) and dead spermatozoa (DMA: 62.1%, DMF: 52.9%, DMSO: 69.2%). After equilibration and after freezing/thawing the best results were obtained in the dimethylformamide environment. Dimethylacetamide and dimethylsulfoxide achieved satisfactory results in freezing semen of Green-Legged Partridge.
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